|Can Baser, K. Husnu|
Submitted to: Journal of Chromatography B
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/18/2006
Publication Date: 12/13/2006
Citation: Tabanca, N., Demirci, B., Can Baser, K., Mincsovics, E., Khan, S.I., Jacob, M.R., Wedge, D.E. 2007. Characterization of Volatile Constituents of Scaligeria tripartita and Studies on the Antifungal Activity Against Phytopathogenic Fungi. Journal of Chromatography B. V. 850:221-229. Interpretive Summary: Scaligeria is represented in Turkey by seven species of which two are native and very little work has been carried out on this genus. Chemical composition of the essential oils obtained from stems and leaves, fruits and roots of Scaligeria tripartita oil was analyzed by gas chromatography-mass spectrometry. A total of 38 compounds were identified ranging 89 to 94% of the oil samples. Oils were subsequently evaluated for their antimalarial, antimicrobial and antifungal activities. The performance of Overpressured Layer Chromatography (OPLC) and Thin Layer Chromatography (TLC) for the analysis of Scaligeria essential oils was also compared. OPLC is a unique liquid chromatography technology system using a planer format. OPLC was described as a bridge between TLC and high performance liquid chromatography (HPLC). We found that OPLC is especially well-suited for the separation of essential oils.
Technical Abstract: The chemical composition of the essential oils obtained from stems and leaves, fruits and roots of Scaligeria tripartita oil was analyzed by gas chromatography-mass spectrometry. A total of 38 compounds were identified ranging 89 to 94% of the oil samples. Geijerene was found as a main compound in the oils of the stems and leaves (37%) and fruits (55%), whereas epoxypseudoisoeugenol angelate (37%) was found as a main compound in the root oil. Oils were subsequently evaluated for their antimalarial, antimicrobial and antifungal activities. Oils obtained from stems and leaves, fruits and roots had good activity against Mycobacterium intracellulare with IC50 values of 15, 8.5, 10 ug/mL, respectively. Scaligeria oils showed no antimalarial activity against Plasmodium falciparum chloroquine-sensitive and resistant clones. Antifungal activity of Scaligeria oils was observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides using the direct overlay bioautography assay. Chemotaxonomically important pure compounds indicated in the bioautography assay were subsequently evaluated in a 96-well microdilution broth assay against Phomopsis obscurans, P. viticola, Fusarium oxysporum, Colletotrichum species and Botrytis cinerea. Epoxypseudoisoeugenol angelate showed 99.9% growth inhibition of C. fragariae at 30 uM and was the most active compound against P. obscurans causing 98.2% and 100% growth inhibition at 3.0 and 30 uM, respectively, and produced inhibition equal to the commercial fungicide azoxystrobin. The performance of OPLC (Overpressured Layer Chromatography) and TLC for the analysis of Scaligeria essential oils was also compared.