Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/22/2006
Publication Date: 7/22/2006
Citation: Chao, S. 2006. Application of molecular marker technologies on cereal crops improvement. American Oat Workers Conference, July 23-25, Fargo, ND. Interpretive Summary:
Technical Abstract: The use of PCR-based DNA markers has greatly improved the efficiency in mapping agronomic traits. One such DNA marker is SSR or microsatellite. It is multiallelic and capable of detecting higher levels of polymorphism. At the moment, SSR is the most extensively used marker system in both wheat and barley. SNP (single nucleotide polymorphism), a biallelic marker system, is newly emerging and will become the next generation of marker in cereal crops. SNPs are numerous in plant genomes and development of SNP markers is currently underway in both wheat and barley. The development of a high-throughout analytical platform and the potential for multiplexing analysis have further increased the efficiency and effectiveness of using PCR-based markers for both genetic studies and breeding practice. High throughput genotyping will facilitate the mapping of agronomic traits through the use of association mapping strategies, which can complement the traditional linkage mapping analysis. Identification of robust markers linked to important agronomic traits is critical for marker-assisted breeding practice. In addition to the availability of robust markers, high throughput genotyping cost and breeding strategies, such as population size, number of traits/markers screened, and selection strategies, should also be taken into consideration in order to efficiently implement marker-assisted selection in the practical cereals breeding programs.