Submitted to: International Conference on Legume Genomics and Genetics
Publication Type: Proceedings
Publication Acceptance Date: 4/9/2006
Publication Date: 4/9/2006
Citation: Hernandez, G., Graham, M., Ramirez, M., Tesfaye, M., Valdes-Lopez, O., Lara, M., Vance, C.P. 2006. Transcript profile of symbiotic nitrogen fixation and root adaptation to phosphorus deficiency in Phaseolus vulgaris. In: Genes to crops. 3rd International Conference on Legume Genomics and Genetics, April 9-13, 2006, Brisbane, Queensland, Australia. p. 43. Interpretive Summary:
Technical Abstract: We have recently initiated transcript profiling of roots and nodules of bean plants grown under phosphorus starvation (-P) as compared to those grown in normal P (+P). Macroarrays spotted with some 4,000 ESTs from the root cDNA library were hybridized with cDNA synthesized from RNA isolated from roots or nodules of bean plants grown under -P vs. +P conditions. Statistical analysis of the signal intensity from each EST in the macroarray revealed differentially expressed ESTs in -P vs. +P roots (93 ESTs) and nodules (112 ESTs). The expression of 43 ESTs was increased in roots and that of 52 ESTs was increased in nodules from P-deprived plants as compared to normal (+P) plants. We also performed a bioinformatics analysis using the public data bases of cDNA libraries or microarray data from plants grown under P-starvation, to determine those ESTs that are significantly over-represented in -P cDNA libraries of bean, soybean, lupin, Medicago, and Arabidopsis. We found that several of the ESTs that are over-expressed in P-starved bean plants, as revealed by macroarray analysis, were the same found to be over-represented in ESTs derived from P-stressed libraries and an Arabidopsis -P whole genome analysis.