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Title: EMBRYO RESCUE AND MERISTEM CULTURE TECHNIQUES USED IN THE DEVELOPMENT OF OAT/MAIZE ADDITION LINES

Author
item GALATOWITSCH, M - UNIVERSITY OF MINNESOTA
item HUETTL, P - UNIVERSITY OF MINNESOTA
item JACOBS, M - UNIVERSITY OF MINNESOTA
item PHILLIPS, R - UNIVERSITY OF MINNESOTA
item Rines, Howard

Submitted to: Society for In Vitro Biology Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/20/2006
Publication Date: 7/6/2006
Citation: Galatowitsch, M.W., Huettl, P.A., Jacobs, M.S., Phillips, R.L., Rines, H.W. 2006. Embryo rescue and meristem culture techniques used in the development of oat/maize addition lines [abstract]. Society for In Vitro Biology Proceedings, June 3-7, 2006, Minneapolis, Minnesota. p. 2046.

Interpretive Summary:

Technical Abstract: Oat/maize addition lines (OMAs) are generated by crossing oat (Avena sativa) and corn (Zea mays) and promoting the growth and development of resulting embryos on a simple culture medium in the absence of normal endosperm development. This process has resulted in the generation of oat plants retaining one or more corn chromosomes. Factors important in promoting embryo and subsequent plantlet development include: oat and corn genotypes involved in crosses, composition and timing of hormone application to pollinated plants, and adequate air exchange of culture vessels. OMA plants generated through embryo rescue exhibit a range of variation in fertility and morphology. Self-fertile addition lines have been recovered for each of the ten individual maize chromosomes; however, the influence of corn and oat genotype effects has hampered efforts to recover complete sets of fertile individual chromosome additions from the elite maize inbreds B73 and Mo17. OMA plants can be maintained in vitro via meristem culture. This technique facilitates the recovery of lines which have relatively poor vigor or fertility and provides an alternative source for generating material for DNA extraction and analysis. Meristems proliferate under low light or darkness on a MS medium with 3 mg/l BA + 0.5 mg/l 2,4-D. Sucrose or maltose are utilized as a carbon source. Meristems have been isolated from plants at a variety of stages in development. These include terminal meristems from plantlets in vitro, terminal and lateral meristems from plants grown under short-day conditions, and lateral and immature floral meristems from long-day grown plants. The immature floral meristems cultured in vitro revert to the vegetative state. Ongoing experiments are centered on developing addition lines with chromosomes from maize inbreds B73 and Mo17.