Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/5/2005
Publication Date: 12/15/2005
Citation: Yocum, G.D., Coudron, T.A., Brandt, S.L. 2005. Diet development for predatory pentatomidae using a nutrigenomics approach [abstract]. Annual Meeting of the Entomological Society of America. p. 36.
Technical Abstract: Recent developments in the area of nutrigenomics hold the promise of providing valuable information about the impact of nutrition on a wide range of biochemical parameters by investigating how nutrition alters global gene expression patterns. Our goal is to use a nutrigenomics approach to identify insect molecular markers that could be used as early indicators of insect responses to different nutritional sources. In our current study Perillus bioculatus, was reared on an optimal versus sub-optimal diet, and then analyzed for the presence of differentially-expressed genes using microarray analysis and suppressive subtractive hybridization. Four differential expressed genes were isolated. A BlastX search found similarities for two diet-upregulated clones, i.e., the tyrosine-3-monooxygenase gene and the gene for the chitin binding protein, Gasp. The probe generated from the tyrosine-3-monooxygenase clone hybridized to two transcripts 2.3 and 1.2 kb in size. The two transcripts were differentially regulated: the 2.3 kb transcript was upregulated in the first and late third instar diet-fed nymphs, whereas the 1.2 kb transcript was upregulated in the second and early third instar diet-fed nymphs. The Gasp gene was upregulated in late third instar nymphs. A positive correlation was found between levels of expression of the isolated genes and the number of generations the insects had been reared on the artificial diet.