Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/2005
Publication Date: 7/30/2005
Citation: Lai, Z., Xu, S.S., Friesen, T.L. 2005. Karotype analysis of Pyrenophora teres f. teres using telomere analysis pulse field gel electrophoresis and the germ tube burst method. American Phytopathological Society Abstracts. Vol. 95:S55.
Technical Abstract: Pyrenophora teres f. teres is an ascomycetous fungus that causes net blotch on barley, a serious foliar disease throughout the world. In order to obtain an accurate karyotype of this fungus, a combination of different methods, including pulse field gel electrophoreses, telomere probe hybridization, and the germ tube burst method (GTBM) were used. P. teres f. teres isolates 15A and 0-1 were assessed in this study. Pulse field gel electrophoreses showed that isolate 15A had seven chromosomal sized bands and isolate 0-1 had six bands, telomere probe hybridization indicated that 15A had at least 8 chromosomes and 0-1 had at least 6 chromosomes, and the GTBM showed a minimum chromosome number of eight each for isolates 15A and 0-1.