Skip to main content
ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #181600
Title: KAROTYPE ANALYSIS OF PYRENOPHORA TERES F. TERERS USING TELOMERE ANALYSIS PULSED FIELDGEL ELECTROPHORESIS AND THE GERM TUBE BURST METHOD.

Author
item LAI, Z.
item Xu, Steven
item Friesen, Timothy

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/2005
Publication Date: 7/30/2005
Citation: Lai, Z., Xu, S.S., Friesen, T.L. 2005. Karotype analysis of Pyrenophora teres f. teres using telomere analysis pulse field gel electrophoresis and the germ tube burst method. American Phytopathological Society Abstracts. Vol. 95:S55.

Interpretive Summary:

Technical Abstract: Pyrenophora teres f. teres is an ascomycetous fungus that causes net blotch on barley, a serious foliar disease throughout the world. In order to obtain an accurate karyotype of this fungus, a combination of different methods, including pulse field gel electrophoreses, telomere probe hybridization, and the germ tube burst method (GTBM) were used. P. teres f. teres isolates 15A and 0-1 were assessed in this study. Pulse field gel electrophoreses showed that isolate 15A had seven chromosomal sized bands and isolate 0-1 had six bands, telomere probe hybridization indicated that 15A had at least 8 chromosomes and 0-1 had at least 6 chromosomes, and the GTBM showed a minimum chromosome number of eight each for isolates 15A and 0-1.