|Rajasekaran, Kanniah - Rajah|
|MC LACHLAN, JOHN|
Submitted to: Society for In Vitro Biology Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2005
Publication Date: 6/30/2005
Citation: Boue, S.M., Kale-Ireland, S., Khan, S., Rajasekaran, K., Shih, B.Y., Carter-Wientjes, C.H., McLachlan, J., Cleveland, T.E. 2005. Response of soybean cell cultures to biological elicitors for the production of antiestrogenic phytoalexins. Society for In Vitro Biology Meeting, June 2005, Baltimore, MD. 40:19-A.
Technical Abstract: Soybean products are known for their high protein content and their production of isoflavones (natural products), many of which have an estrogenic activity. Interestingly, soy plants also display a natural resistance to Aspergillus flavus and A. parasiticus, the fungi which make the highly mutagenic and carcinogenic secondary metabolites, the aflatoxins. Since the aflatoxigenic fungi routinely infect crops of human and animal consumption but the soybean plant is able to avoid this infection, it is possible that the soybean cells release stress-response compounds, mainly isoflavones called phytoalexins, which offer a protective role to avoiding this infection. Some of these phytoalexins, including genistein and daidzein, have been shown to be beneficial to human health. In this study the ability of several elicitors at inducing phytoalexins in soybean liquid cell culture were evaluated. Of particular interest was the ability of each elicitor to induce the isoflavone phytoalexin glyceollin, which has recently been shown to be antiestrogenic in several in vitro assays. Soybean cells from both control and elicitor-exposed cultures were analyzed using HPLC-MS. Several constitutive isofavones have been identified and preliminary data indicate novel phytoalexins related to the glyceollins are formed.