Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2004
Publication Date: 1/15/2005
Citation: Luo, M., Holbrook, Jr., C. C., Lee, R. D., Bausher, M. G., Guo, B. 2005. Identification of resistance genes in a peanut genotype against Aspergillus flavus infection and drought stress [abstract]. In: Proceedings of the Plant and Animal Genome XIII Conference, January 15-19, 2005, San Diego, CA. p. 740. Interpretive Summary:
Technical Abstract: Aflatoxin contamination caused by Aspergillus fungi is a great concern in peanut production world wide. Pre-harvest A. flavus infection usually happens in peanuts that are grown under less-irrigation, drought stressed condition. However, drought resistant peanut lines displayed less aflatoxin contamination under the same situation. In this research, we used microarray, which partly came from the A. flavus and drought induced cDNA library for resistant genotype A13, to screen the genes induced by these stresses. About 83 up-regulated spots (Log2 ratio>1) under the fungal and drought stresses, and 104 up-regulated spots in drought stress were detected by microarray analysis. Among the total up-regulated spots, forty-nine spots had positive results in both treatments. The top 20 genes expressed under the two stresses were chosen to be validated by real-time PCR for the expression levels and the association with the resistance. Some genes with known function of drought tolerance or disease resistance in other plants were also expressed differentially in the peanut genotype A13 under the A. flavus inoculation and drought stress. These genes will be studied further in peanut for the functional characterization.