Submitted to: Phycological Society of America
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2004
Publication Date: 8/1/2004
Citation: Zimba, P.V., Weaver, M.A., Sullivan, M.J., Czarnecki, D. 2004. Fatty acid analyses of 8 craticula cuspidate clones. J. Phycology 41(suppl.):73.
Technical Abstract: Fatty acid profiles have been used to elucidate food webs, and to assess evolutionary adaptations within plant genera. We raised clonal isolates of Craticula cuspidata (originally isolated from eight locations in temperate North America) axenically and analyzed late exponentially growing cells for fatty acid composition. Cultures were grown at both 20 and 27C. Samples were concentrated on filters, and processed for fatty acid analyses using a gas chromatograph equipped with gas capillary and flame ionization detection. A reference library (MIDI) was used to facilitate identification. Percent contribution of saturated fatty acids was generally higher at 27C, whereas cultures grown at 20C had more unsaturated fatty acids. Palmitic acid, a saturated fatty acid, and 16-carbon unsaturated forms were the most abundant fatty acids present.