Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2003
Publication Date: 1/31/2004
Citation: Luo, M., Dang, P., Liang, X.Q., Holbrook, C.C., Guo, B.Z. 2004. Identification of resistance related genes in peanut using microarray analysis [abstract]. Plant and Animal Genome Conference XII, January 11-15, 2004, San Diego, CA. p. 882.
Technical Abstract: Peanut (Arachis hypogaea L.) is one of the principle legume crops globally valued for its protein, oil content, and quality. The major issues in the southeastern U.S. are food safety of aflatoxin contamination and leaf diseases of leaf spots. They are quantity traits. To identify important resistance related genes and understand the resistance mechanisms against these diseases in peanut, two induced cDNA libraries from leaf (C34-24) and immature pod (A13) were constructed, and thousands of ESTs were sequenced. Functional categories and redundancy of the ESTs were analyzed, and several high redundant disease-resistance-related (DRR) ESTs were found. The first batch of ESTs 1345 has been submitted to GenBank of NCBI. About 380 clones selected as putative DRR clones from the two libraries were re-arrayed, and used to produce cDNA microarray chips. C34-24 and YY20 were used as resistant and susceptible lines respectively to screen DRR genes by different screening based on the microarray, and several other lines also used to identify the important DRR genes. Real time PCR technique was used to identify the expression difference of target genes in different lines. In this research, we want to list some important resistance genes in the two traits as molecular markers, and use these markers to evaluate germplasm and assistant selection in breeding.