Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/15/2003
Publication Date: 1/10/2004
Citation: Phillips, R.L., Rines, H.W., Kynast, R.G., Okagaki, R.J., Galatowitsch, M.W., Jacobs, M.S., Stec, A.O., Schmidt, C.M., Retzel, E.F., Odland, W.E. 2004. Chromosome dynamics in oat-maize addition lines and derivatives [abstract]. Plant and Animal Genome XII Abstracts. Paper No. W190.
Technical Abstract: Oat x maize crosses yield occasional embryos that can be in vitro rescued. The resulting plantlets are oat haploids containing from 0 to 10 maize chromosomes. Unreduced gamete formation driven by meiotic restitution produces offspring that are doubled haploids of oat with about one-third possessing a single maize chromosome, usually as a disomic addition. From more than 80,000 oat x maize hybridizations, about 60 disomic maize addition lines have been recovered. These include at least one for each of the ten chromosomes of maize except chromosome 10; for it to date we have a disomic addition only for the short arm telosome. Several features of the oat-maize addition lines vary with the genetic background of oat and maize and in interaction with the growing conditions. Seneca 60 maize provided the highest number of oat-maize addition lines. Although most crosses were made with Starter as oat parent, we obtained fertile additions for maize chromosomes 3 and 10 only in other oat backgrounds. Transmission of the added maize chromosome 1 depends on the genetic background. The liguleless phenotype of chromosome 3 addition (Sun II background) is suppressed in crosses to Starter oat. The chromosome 10 addition in GAF/Park oat background is sterile, whereas in Sun II seed can form and transmit the short arm of chromosome 10. Screening for disease resistance among the addition lines has revealed that a chromosome 5 addition provides some oat crown rust resistance. This research is supported by the National Science Foundation under Grant No. 0110134.