Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/15/2004
Publication Date: 1/10/2004
Citation: Jacobs, M., Okagaki, R., Kynast, R., Odland, W., Galatowitsch, M., Schmidt, C., Stec, A., Suresh, J. Dosdell, A., Huett, P., Ninkovic, J., Stevenson, E.,Retzel, E., Rines, H.W., Phillips, R. 2004. Adaptation of high throughput techniques for the development and use of oat-maize radiation hybrid mapping panels [abstract]. Plant and Animal Genome XII Conference Abstracts. p. 490. Interpretive Summary:
Technical Abstract: The goal of our project is to generate and use chromosome-specific radiation hybrid (RH) maps with high physical resolution for each of the ten different maize chromosomes. We are developing the RH maps from DNA panels of oat plants that carry segments of single maize chromosomes in the form of added maize chromosomes with deletions or oat-maize translocations. Screening for useful RH lines requires thousands of PCR assays, and this has hindered our progress. Last year it took two weeks to do the 10,000 PCR assays to characterize the chromosome 6 RH lines. One person can now do this in less than two days without robotics. With the aid of these techniques, we now have mapping panels allowing marker assignment to approximately 10 segments along each of chromosomes 1, 2, 4, 6 and 9. EST and GSS sequences are being mapped on these panels. Seed and DNA is being produced for distribution. With additional RH lines available for chromosomes 6 and 9 we are developing approaches that will support higher resolution mapping. This material is based upon work supported by the National Science Foundation under Grant No. 0110134.