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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Cotton Structure and Quality Research » Research » Publications at this Location » Publication #154110
Title: AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION OF COTTON

Author
item Rajasekaran, Kanniah - Rajah

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 10/30/2003
Publication Date: 2/15/2004
Citation: Rajasekaran, K. 2004. Agrobacterium-mediated genetic transformation of cotton. In: Curtis, I.S., editor. Transgenic Crops of the World - Essential Protocols. The Netherlands: Kluwer Academic Publishers. p. 243-254.

Interpretive Summary:

Technical Abstract: The commercially available genetically modified cottons with insect-resistance and/or herbicide-tolerance traits are the products of Agrobacterium-mediated transformation. Although this method is applicable to a broad range of species and varieties, the success is limited by low regeneration rates with commercially grown varieties. A detailed, reproducible procedure for Agrobacterium-mediated transformation and regeneration of fertile plants has not been published to date. This book chapter provides a step-by-step procedure on cotton transformation and regeneration. This book is to be used as a practical manual by students and scientists alike for genetic transformation of crop plants.