Submitted to: National Fusarium Head Blight Forum Proceedings
Publication Type: Abstract only
Publication Acceptance Date: 11/1/2002
Publication Date: 12/7/2002
Citation: AGRAMA, H.A., DAHLEEN, L.S., HORSLEY, R.D., STEFFENSON, B.J., SCHWARZ, P.B., MESFIN, A., FRANCKOWIAK, J.D. QTL ANALYSIS OF FUSARIUM HEAD BLIGHT IN BARLEY USING THE CHINESE LINE ZHEDAR 2.. NATIONAL FUSARIUM HEAD BLIGHT FORUM PROCEEDINGS. 2002. Abstract p. 2. Interpretive Summary:
Technical Abstract: Fusarium head blight (FHB) in barley and wheat caused by Fusarium graminearum is a continual problem worldwide. Primarily, FHB reduces yield and quality and produces the toxin deoxynivalenol (DON), which can affect food safety. Locating QTLs for FHB severity, DON level and related traits heading date (HD) and plant height (HT) with consistent effects across a set of environments would increase the efficiency of selection for resistance. A population of seventy-five double haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster, was used for genome mapping and FHB evaluation. Phenotypic data were collected in replicated field trails from five environments in two growing seasons. A linkage map of 214 RFLP, SSR and AFLP markers was constructed. The data were analyzed using MQTL software to detect QTL x environment interaction. Because of the presence of QTL x E, the MQM in MAPQTL was applied to identify QTLs in single environments. MQM mapping identified nine QTLs for FHB severity and five for low DON. Only three of these QTLs were consistent across environments. Five QTLs were associated with HD and two with HT. Regions that appear to be promising candidates for MAS and further genetic analysis including the two FHB QTLs on chromosome 2H and one on 6H which also were associated with low DON and later heading date in multiple environments. This study provides a starting point for manipulating Zhedar 2-derived resistance by MAS in barley to develop varieties that will show effective resistance under disease pressure.