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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #148429
Title: CHARACTERIZATION OF PHOSPHATE DEFICIENCY INDUCED GENES FROM WHITE LUPIN AND ANALYSIS OF PROMOTER ACTIVITY IN TRANSGENIC PLANTS

Author
item LIU, JUNQI - UNIV OF MINNESOTA
item BUCCIARELLI, BRUNA - UNIVERSITY OF MINNESOTA
item HATLESTAD, GREGORY - UNIVERSITY OF MINNESOTA
item Samac, Deborah - Debby
item Vance, Carroll
item ALLAN, DEBORAH - UNIV OF MINNESOTA

Submitted to: International Symposium on Phosphorus Dynamics in the Soil Plant Continuum
Publication Type: Abstract Only
Publication Acceptance Date: 6/13/2003
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Phosphorus (P) is essential for plant growth and development. Its deficiency leads to the activation of genes with various functions in enhancing P uptake and utilization. Phosphate transporter and acid phosphatase genes are two important P-deficiency induced genes in higher plants. We previously reported the cloning of a phosphate transporter gene LaPT1 and an acid phosphatase gene LaSAP1 from white lupin (Lupinus albus) and demonstrated enhanced gene expression in response to P deficiency. Here we provide further details of the temporal and spatial pattern of LaPT1 and LaSAP1 promoter activity by introducing promoter::GUS reporter gene fusions into another legume species, alfalfa (Medicago sativa). Both LaPT1::GUS and LaSAP1::GUS reporter genes showed significant induction by P deficiency in transgenic alfalfa. In addition, we also characterized a MYB gene whose expression seems to be modulated by exogenous phosphate at early developmental stages. A MYB::GUS reporter was also introduced in alfalfa and the transgenic plants are being analyzed.