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ARS Home » Southeast Area » Raleigh, North Carolina » Food Science Research » Research » Publications at this Location » Publication #141326
Title: ZONIERUNG VON INTAKTEN BAKTERIOPHAGEN

Author
item BARRANGOU, R - NCSU
item Breidt, Frederick
item SCHROEN, D - KENDRO NEWTOWN CT

Submitted to: Git Labor-Fachzeitschrift
Publication Type: Research Notes
Publication Acceptance Date: 9/1/2002
Publication Date: 10/1/2002
Citation: Barrangou, R., Breidt, F., Schroen, D. 2002. Zonierung von intakten bakteriophagen. Git Labor-Fachzeitschrift.4:470-471.

Interpretive Summary: With recent advances in molecular biology, the isolation of bacteriophage continues to increase. The SORVALL Discovery M150 micro-ultracentrifuge allows rapid concentration and purification of bacteriophage in a CsCl step gradient. The pure phage solution obtained via ultracentrifugation can be subsequently used for electron microscopy and protein or nucleic acid analysis. This article originated as Banding of Intact Bacteriophage Using the S100-AT6 Rotor and Discovery M150 Micro-ultracentrifuge and appeared in a Kendro Sorvall Application Brief published by Kendro Laboratory Products, Newton, CT.

Technical Abstract: With recent advances in molecular biology, the isolation of bacteriophage continues to increase. The SORVALL Discovery M150 micro-ultracentrifuge allows rapid concentration and purification of bacteriophage in a CsCl step gradient. The pure phage solution obtained via ultracentrifugation can be subsequently used for electron microscopy and protein or nucleic acid analysis. This article originated as Banding of Intact Bacteriophage Using the S100-AT6 Rotor and Discovery M150 Micro-ultracentrifuge and appeared in a Kendro Sorvall Application Brief published by Kendro Laboratory Products, Newton, CT.