|Holbrook, Carl - Corley|
Submitted to: Mycopathologia
Publication Type: Abstract Only
Publication Acceptance Date: 9/10/2002
Publication Date: 10/15/2002
Citation: Luo, M., Dang, P. M., Guo, B., Lee, R. D., Holbrook, Jr., C. C., Niedz, R. P., Lynch, R. E. 2002. Gene expession profiling in kernel developing stages as influenced by drought stress [abstract]. In: Proceedings of the 2nd Fungal Genomics, 3rd Fumonisin and 15th Aflatoxin Elimination Workshop, October 23-25, 2002, San Antonio, Texas. p. 137.
Technical Abstract: Preharvest aflatoxin contamination in corn and peanut is a severe problem in the southern U.S. The previous researches showed genetic differences in drought tolerance and the positive correlation between drought tolerance and reduced preharvest aflatoxin contamination in corn and peanut. The overall goals of this project are to better understand global gene expression profiling of drought tolerance at certain stages of seed development under stress of water deficiency, and to identify the biochemical pathways and genes controlling the resistance to Aspergillus flavus infection and aflatoxin contamination. The peanut variety A13 and inbred lines of GT-MAS:gk will be used in this project. Two cDNA libraries have been constructed for EST/microarray analysis. Some ESTs from peanut libraries have been sequenced, and there are 215 of 361 high quality ESTs that functions can be identified by BLASTx analysis. The preliminary ESTs show that some plant defense genes have been identified, such as heat-shock protein, Ca2+/H+-exchanging protein, peroxidase, small cysteine-rich antifungal protein, and ribosomal protein. We have full-length sequence of ribosomal protein gene identified by DD-RT-PCR and cloned by RACE.