Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 9/26/2002
Publication Date: 9/26/2002
Citation: PHILLIPS, R.L., RINES, H.W., OKAGAKI, R.J., KYNAST, R.G., DONAHUE, R., ODLAND, W.E. GENETIC AND PHYSICAL MAPPING OF THE CORN GENOME THROUGH RADIATION HYBRIDS. INTERNATIONAL RICE RESEARCH CONFERENCE. 2002. ABSTRACT. P. 34.
Technical Abstract: Chromosome addition lines and radiation hybrids (RHs) provide powerful mapping tools. Oat x corn crosses followed by embryo rescue results in haploid oat plants with one or more corn chromosomes. Seed and/or DNA are available of monosomic or disomic additions of each of the ten corn chromosomes in an oat background. These materials are especially useful for mapping DNA sequences to maize chromosome. Irradiation of monosomic addition line seed results in plants and progeny with various corn chromosome modifications allowing physical mapping to a 5 Mb region or smaller. Use of a dispersed corn-specific retroelement, chromosome-specific markers, and genomic in situ hybridization (GISH) on interphase cells speeds the identification of corn chromosomes and chromosome segments. GISH has been used recently to distinguish various genomes of rice. Monosomic alien addition lines have been produced between Oryza sativa L. and several of the wild rice species. These materials also will simplify certain genetic procedures. Addition lines often have distinctive phenotypes. Correlating the phenotype and corn chromosome segments in RHs allows placement of the gene(s) controlling the trait. Chromosome 6 oat-corn addition exhibits a disease lesion mimic phenotype; data from chromosome 6 RHs indicate the phenotype may be controlled by the Les13 gene located in 6L.