Author
Klich, Maren | |
Mullaney, Edward |
Submitted to: Book Chapter
Publication Type: Book / Chapter Publication Acceptance Date: 8/28/2002 Publication Date: 1/1/2004 Citation: Klich, M.A., Mullaney, E.J. 2004. Molecular methods for identification of plant pathogenic fungi. In: Arora, D.K., Bridge, P.D., Bhatnagar, D., editors. Fungal Biotechnology in Agricultural, Food and Environmental Applications. New York, NY: Marcel Dekker. p. 49-55. Interpretive Summary: Technical Abstract: The advent of molecular biology has provided many new tools for fungal taxonomists. In this review, a number of DNA methodologies used by taxonomists are discussed, including: restricition fragment length polymorphisms; variable number tandem repeats; microsatellites; and a number of methods based on the use of the polymerase chain reaction. Recent applications of these methods in identification of fungal plant pathogens are provided. The advantages and disadvantages of molecular applications in vivo versus in vitro are discussed, as are the limitations of molecular applications in plant pathology. |