Author
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Rines, Howard |
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Submitted to: Book Chapter
Publication Type: Book / Chapter Publication Acceptance Date: 8/1/2002 Publication Date: 11/15/2003 Citation: RINES, H.W. OAT HAPLOIDS FROM WIDE HYBRIDIZATION. MALUSZYNSKI, M., KASHA, K.J., FORSTER, B.P., SZAREJKO, I., EDITORS. KLUWER ACADEMIC PUBLISHERS, DORDRECHT, NETHERLANDS. DOUBLED HAPLOID PRODUCTION IN CROP PLANTS. A MANUAL.2002. P. 155-160. Interpretive Summary: Technical Abstract: Haploids of cultivated oat (Avena sativa L., 2n = 6x = 42) may be produced from wide hybridizations with Panicoidae species, particularly maize (Zea mays L., 2n = 2x = 20). Haploid oat production by the maize wide cross method appears to be less genotype restricted than haploid production by anther culture; however, the plant recovery frequencies reported to date of 1-2% of florets maize-pollinated is low like that for oat haploid production by oat anther culture and not yet adequate for routine use in breeding. The oat x maize hybridization results in novel features in the types and in the reproductive behavior of plants recovered. These include maize chromosome retention in a portion of the recovered oat plants and partial self-fertility in oat haploid plants. These differences in products can be detrimental in trying to routinely produce doubled haploids for breeding, but on the plus side they have led to the recovery of valuable materials for genetic and genomic studies in both oat and maize. This report details protocols currently in use for recovery and for molecular and cytological characterization of doubled haploid oat plants, both with and without added maize chromosomes, from oat x maize hybridizations and describes features of derived plants that make them novel and valuable. |
