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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #133923
Title: OAT X MAIZE CROSSES AS A SOURCE OF GENOMIC TOOLS AND NOVEL GERMPLASM

Author
item Rines, Howard
item PHILLIPS, RONALD - UNIV OF MINNESOTA
item KYNAST, RALF - UNIV OF MINNESOTA
item OKAGAKI, RON - UNIV OF MINNESOTA

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/5/2002
Publication Date: 5/5/2002
Citation: RINES, H.W., PHILLIPS, R.L., KYNAST, R.G., OKAGAKI, R.J. OAT X MAIZE CROSSES AS A SOURCE OF GENOMIC TOOLS AND NOVEL GERMPLASM. AMERICAN OAT WORKERS CONFERENCE PROCEEDINGS. 2002. ABSTRACT. P. 18.

Interpretive Summary:

Technical Abstract: Pollination of emasculated oat florets with maize pollen followed by in vitro rescue of embryos leads to the recovery of both haploid oat plants (2n = 3x = 21) and plants with one or more maize chromosomes added to a complete haploid oat genome. From about 80,000 oat florets pollinated, 410 plants have been recovered. Of these, 271 had only oat chromosomes. Haploid oat plants were partially self-fertile via formation of unreduced gametes producing doubled haploid progeny. The euploid doubled haploids recovered among these offspring or from colchicine-doubled sectors of haploids have special value as germplasm in genetic and breeding studies. Among the 410 recovered oat x maize progeny, 139 had added maize chromosomes. Plants with only one or on occasion two maize chromosomes were frequently partially self-fertile and transmitted the maize chromosome(s). Fertile disomic additions of individual maize chromosomes (2n = 2x + 2 = 44) have been recovered for each of the ten chromosomes of maize except for chromosome 10. Phenotypic effects depending on the particular maize chromosome present were observed and included changes in plant morphology, fertility, and day-length response, and the appearance of lesions. Serving as tools in functional genomic studies the addition lines can be used to analyze oat x maize gene interactions and can be intercrossed to analyze interactions between individual maize chromosomes isolated from the remainder of the maize genome. To be determined is if the added maize genetic material may provide traits useful to oat improvement such as disease resistance or heat tolerance. A portion of this work was supported by National Science Foundation under Grant No. 0110134.