Skip to main content
ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #133921
Title: MOLECULAR MARKERS FOR OAT CROWN RUST PARTIAL RESISTANCE QTLS IN MN841801-1

Author
item PORTYANKO, VLADIMIR - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA
item DIAZ, JUAN - UNIVERSITY OF MINNESOTA
item STUTHMAN, DEON - UNIVERSITY OF MINNESOTA

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/5/2002
Publication Date: 5/5/2002
Citation: PORTYANKO, V.A., RINES, H.W., PHILLIPS, R.L., DIAZ, J.E., STUTHMAN, D.D. MOLECULAR MARKERS FOR OAT CROWN RUST PARTIAL RESISTANCE QTLS IN MN841801-1. AMERICAN OAT WORKERS CONFERENCE PROCEEDINGS. 2002. ABSTRACT. P. 42.

Interpretive Summary:

Technical Abstract: The observed ability of the oat crown rust pathogen Puccinia coronata to rapidly overcome high-level race-specific host resistance through population virulence shifts has led to a strong interest in breeding oats with partial, race non-specific resistance. One way to obtain this possibly more durable resistance is to introgress into elite germplasm lines genes for partial adult plant resistance through marker-assisted selection. Previously, we reported the identification of five QTLs for crown rust partial resistance in analysis of a recombinant inbred population produced from a cross of MN841801, a line with effective adult plant partial resistance, and the susceptible cultivar 'Noble'. The objectives of the current studies were 1) to identify or develop PCR-based markers appropriate for high-throughput marker-assisted selection, and 2) to test for QTLs in two additional populations sharing MN841801-1 as a donor of partial resistance but with elite breeding lines with some resistance as the other parents. A microsatellite marker, AM3, was found to be associated with a QTL on linkage group MN26. Insertion/deletion (Indel) variants allowed design of PCR-based markers, two of which have been mapped near detected rust resistance QTLs on MN12 and MN14, respectively. Tests involving two years of field rust severity data combined with marker polymorphism for three QTL regions in an MN841801-1 x Richard population detected two QTLs with MN841801-1 as the positive allele donor and one with Richard as donor. No significant effects of markers for three tested regions were found in the MN841801-1 x MN95201 population in the single year it was tested. Future efforts will include additional testing of the populations, further PCR-based marker development, and derivation of near-isogenic sib lines to confirm allele effects in the MN841801-1 x Noble-2 population.