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ARS Home » Midwest Area » St. Paul, Minnesota » Plant Science Research » Research » Publications at this Location » Publication #131859
Title: LOW AND MEDIUM RESOLUTION RADIATION HYBRID MAPS FOR MAIZE CHROMOSOMES 2 AND 9

Author
item OKAGAKI, RON - UNIVERSITY OF MINNESOTA
item KYNAST, RALF - UNIVERSITY OF MINNESOTA
item SCHMIDT, CHRISTINA - UNIVERSITY OF MINNESOTA
item ODLAND, WADE - UNIVERSITY OF MINNESOTA
item RUSSELL, CHARLES - UNIVERSITY OF MINNESOTA
item STEC, ADRIAN - UNIVERSITY OF MINNESOTA
item ZAIA, HERIKA - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2002
Publication Date: 3/14/2002
Citation: OKAGAKI, R.J., KYNAST, R.G., SCHMIDT, C.M., ODLAND, W.E., RUSSELL, C.D., STEC, A.O., ZAIA, H.A., RINES, H.W., PHILLIPS, R.L. LOW AND MEDIUM RESOLUTION RADIATION HYBRID MAPS FOR MAIZE CHROMOSOMES 2 AND 9. 44TH MAIZE GENETICS CONFERENCE. 2002. ABSTRACT. P. 80.

Interpretive Summary:

Technical Abstract: The goal of the radiation hybrid mapping project is to produce chromosome specific high resolution radiation hybrid maps for the maize chromosomes. Maps are being developed from oat plants that carry single maize chromosome segments generated from monosomic oat-maize chromosome addition lines by radiation. Our efforts have been focused on maize chromosomes 2 and 9 with an interim objective of producing low and medium resolution mapping panels These panels are intended to be used for high throughput mapping of sequences to chromosome segments. We now have a low resolution map for chromosome 9 that divides chromosome 9 into six major segments. We are developing a medium resolution map that divides chromosome 9 into approximately 20 segments. Low and medium resolution maps also are being developed for chromosome 2. We are using these materials to map duplicated genes. Small gene families are identified by grouping EST contigs and singletons into clusters based on BLAST searches. PCR primers are designed against representative sequences from EST clusters. Sequences are first mapped to chromosome using plus/minus PCR assays on chromosome addition lines. Sequences located on chromosomes 2 and 9 are then mapped to segment using radiation hybrid mapping panels. Self-fertile disomic addition lines currently are available for all maize chromosomes except chromosome 10. We have a haploid addition of chromosome 10 with efforts to recover a disomic version to complete the set. DNA is available for all 10 addition lines and the chromosome 9 low resolution mapping lines. This material is based upon work supported by the National Science Foundation under Grant No. 0110134.