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Title: A RADIATION HYBRID SYSTEM FOR THE GENETIC AND PHYSICAL MAPPING OF THE CORN GENOME

Author
item OKAGAKI, R - UNIVERSITY OF MINNESOTA
item KYNAST, R - UNIVERSITY OF MINNESOTA
item ODLAND, W - UNIVERSITY OF MINNESOTA
item STEC, A - UNIVERSITY OF MINNESOTA
item RUSSELL, C - UNIVERSITY OF MINNESOTA
item ZAIA, H - UNIVERSITY OF MINNESOTA
item Rines, Howard
item PHILLIPS, R - UNIVERSITY OF MINNESOTA

Submitted to: Maize Genetics Cooperation Newsletter
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2002
Publication Date: 5/15/2002
Citation: OKAGAKI, R.J., KYNAST, R.G., ODLAND, W.E., STEC, A., RUSSELL, C.D., ZAIA, H.A., RINES, H.W., PHILLIPS, R.L. A RADIATION HYBRID SYSTEM FOR THE GENETIC AND PHYSICAL MAPPING OF THE CORN GENOME. MAIZE GENETICS COOPERATION NEWS LETTER. 2002. V. 76. P. 88.

Interpretive Summary:

Technical Abstract: Oat-maize chromosome addition lines generated by crossing oat with maize provide the starting material for a project to develop a radiation hybrid system for mapping individual maize chromosomes. The major objectives for this project funded by the Plant Genome Research Program are: 1) establish fertile disomic addition lines for each maize chromosome and supply seed and genomic DNA to the scientific community, 2) produce low- and high- resolution radiation hybrid maps, 3) develop high-throughput technologies for mapping ESTs to chromosomal region, and 4) screen these materials for agronomic and genetic traits that may be of interest to the plant research community. All 10 maize chromosomes have been successfully introduced into oat, and fertile disomic lines exist for 9 of the maize chromosomes. DNA is available for all 10 oat-maize chromosome addition lines. Radiation hybrid lines are derived from chromosome addition lines by irradiating seed monosomic for the maize chromosome. This treatment can fragment the maize chromosome and produce radiation hybrid plants that carry deleted maize chromosomes and translocated maize chromosome segments. Low-resolution mapping panels are nearing completion for chromosomes 2 and 9. High- resolution panels will be generated using larger numbers of radiation hybrid lines with a goal to be able to map to a 1-2 Mb region. Efforts are underway to adapt microarray technology for mapping ESTs with chromosome addition lines. Oat-maize addition lines are being screened for characters such as disease resistance and photoperiod response. More information about the project, materials available, traits observed, and publications can be found at: //www.agro.agri.umn.edu/rp/genome/. This work was sponsored by NSF Grant No. 9872650.