Author
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KYNAST, RALF - UNIVERSITY OF MINNESOTA |
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OKAGAKI, RON - UNIVERSITY OF MINNESOTA |
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Rines, Howard |
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PHILLIPS, RONALD - UNIVERSITY OF MINNESOTA |
Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 1/12/2002 Publication Date: 1/12/2002 Citation: KYNAST, R.G., OKAGAKI, R.J., RINES, H.W., PHILLIPS, R.L. BEHAVIOR AND MANIPULATION OF MAIZE CHROMOSOMES ADDED TO OAT. PLANT AND ANIMAL GENOME X CONFERENCE. 2002. ABSTRACT. P. 147. Interpretive Summary: Technical Abstract: Oat-maize additions were recovered for each of the 10 maize chromosomes among F1 plants generated by oat x maize crosses. Fertile disomic additions to hexaploid oat are available for all maize chromosomes except chromosome 10. Besides phenotype descriptions, we report on the transmission and pairing behavior of the added maize chromosomes. Disomic and monosomic additions for chromosomes 2, 3, 4, 6, and 9 transmit as expected for alien additions. Somatic sectoring resulting in irregular transmission has been detected for chromosomes 1, 5, and 8. We discuss genotype interactions and their potential impact on the strategy to complete the production of panels of radiation hybrid (RH) lines for all 10 maize chromosomes. Oat-maize RH lines are generated by gamma-irradiating monosomic seed of oat-maize additions. After screening among their offspring with maize-specific markers, RH plants with maize chromosome deletions and oat-maize translocations are retained for DNA and seed production. We have recovered 191 oat-maize RH plants with maize chromosome 9, 223 plants with chromosome 2, 59 plants with chromosome 4, and 101 plants with chromosome 6. Seeds have been irradiated for developing RH lines for chromosomes 3 and 7. Monosomic seed are being generated for chromosomes 1 and 5. RH lines are used to establish panels for physical mapping of genes and markers in maize. Research is supported under NSF Grant No. 9872650. |