|UHDE STONE, C|
|Samac, Deborah - Debby|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/9/2001
Publication Date: N/A
Technical Abstract: Roots of white lupin in response to -P (phosphorus) stress display highly synchronous changes in development and biochemistry resulting in cluster (proteoid) root formation, exudation of organic acids, release of acid phosphatase, and enhanced P uptake. In efforts to dissect the molecular events mediating these adaptive responses we have isolated and sequenced 2,000 expressed sequence tags (ESTs) from cDNA libraries prepared with RNA isolated at various stages of proteoid root development. Approximately 60% of the ESTs could be identified based upon homology to known sequences through BLAST analysis, while the remaining 33% encoded either proteins of unknown function or had no homology to known sequences. Those ESTs with significant homology to known sequences were categorized into 12 functional categories. Several ESTs were highly redundant within the population. Macroarray experiments allowed us to evaluate global aspects of gene expression in response to -P stress. Upregulated ESTs in response to -P stress included genes involved in: carbon metabolism, nutrient transport, stress response, plant hormone metabolism, and signal transduction. Expression and function data will be described for several ESTs. Novel acid phosphatase and phosphate transporter genes will be presented along with promoter function.