Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 10/21/2001
Publication Date: 10/21/2001
Citation: PORTYANKO, V.A., RINES, H.W., PHILLIPS, R.L., DIAZ, J.E., STUTHMAN, D.D. MOLECULAR MARKERS FOR PARTIAL RESISTANCE TO CROWN RUST IN OAT. AMERICAN SOCIETY OF AGRONOMY MEETINGS. 2001. ABSTRACT. P. 529R.
Technical Abstract: Crown rust, a fungal disease of oat (Avena sativa L.) caused by Puccinia coronata f. sp. avenae, frequently results in serious yield losses. One way to obtain possibly more durable resistance to oat crown rust is to introgress into high performance germplasm genes for partial, adult-plant resistance through marker-assisted breeding. Previously, we reported five oat QTLs for partial resistance to crown rust in a recombinant inbred population MN841801-1 x Noble-2; detection of these QTLs was facilitated by a molecular map based on more than 200 marker loci. The objectives of this study were 1) to develop PCR-based markers appropriate for high-throughput marker-assisted selection, and 2) to validate QTLs detected in two additional populations sharing MN841801-1 as a donor of partial resistance. DNA clones of marker loci most closely linked to each of the five genomic regions with resistance QTLs were sequenced to design allele-specific PCR- based markers. Using the markers generated, the effects of these five QTL regions are being tested in two oat recombinant inbred populations, MN841801-1 x Richard and MN841801-1 x MN95201.