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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Insect Genetics and Biochemistry Research » Research » Publications at this Location » Publication #122930
Title: IDENTIFICATION OF THE HYDROCARBONS IN SURFACE LIPID EXTRACTS OF THE VITELLINE MEMBRANES OF DIPTERAN EMBRYOS

Author
item Nelson, Dennis
item Leopold, Roger

Submitted to: Society for Cryobiology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/14/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Treatment of dechorionated eggs with hexane was used to extract hydrocarbons, wax esters and acylglycerols from the vitelline membranes of flies. Lipid classes were characterized by thin-layer chromatography and hydrocarbon components by gas chromatography-mass spectrometry. Long- chain hydrocarbons were the major lipid class from vitelline membranes. Hydrocarbon size range was: New World screwworm, Cochliomyia hominivorax (C23 - C49), secondary screwworm, Cochliomyia macellaria (C27 - C33), sheep blow fly, Lucilia cuprina (C24 - C35), house fly, Musca domestica (C25 - C36), green bottle fly, Phaenicia sericata (C25 - C33), and Mexican fruit fly, Anastrepha ludens, (C21 - C51). The major hydrocarbon component was nonacosane (C29) in C. hominivorax (40%), C. macellaria (43%), L. cuprina (38%), M. domestica (39%), and P. sericata (60%), but in A. ludens, it was 2-methyloctacosane (32%). The second most abundant components were heptacosane (C27) in C. hominivorax (27%) and P. sericata (13%), hentriacontane (C31) in C. macellaria (32%) and M. domestica (31%), nonacosane (C29) in A. ludens (17%), and 2-methyloctacosane in L. cuprina (10%). None of the embryos had unsaturated hydrocarbons except for A. ludens which had monoenes (~16%) from C30 to C45 and dienes (~11%) from C31 to C45. The major dienes were hentriacontadiene (C31:2; 5%) and tritriacontadiene (C33:2; 4%). The major monoene was hentriacontene (C31:1; about 6%) was estimated as it did not fully resolve from 2- methyltriacontane.