|Samac, Deborah - Debby|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 8/25/2001
Publication Date: 6/1/2001
Citation: LEE, S.D., KINKEL, L.L., SAMAC, D.A. GENETIC DIVERSITY OF STREPTOMYCETE POPULATIONS ASSOCIATED WITH TWO DIFFERENT PRAIRIE SOILS. PHYTOPATHOLOGY. 2001. V. 91. P. 554.
Technical Abstract: The genetic diversity of Streptomycete populations was determined in two prairie soils with different nitrogen-input histories at the Cedar Creek Natural History Area, an NSF-LTER site in central Minnesota. A total of 299 Streptomycete were obtained from 3 different locations and 4 different depths within each soil using specially designed micro-corers. Diversity was assessed by REP-PCR fingerprinting using the BOX A1R primer. Cluster analysis revealed 65 distinct genetic groups, with groups defined at a similarity of 50%. Relative abundance of individual cluster groups varied between the two soils. In both soils, more than 20% of isolates comprised a single genetic group, but the dominant group differed between the two soils. Genetic diversity was greater in the low nitrogen than in the high nitrogen soil. There were substantial differences in community composition in the soil at small spatial scales, including both among locations within a particular soil and between adjacent 2 cm x 5 mm subsamples within micro cores. However, there was no consistent effect of soil depth on community composition.