|Riera lizarazu, O|
Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract only
Publication Acceptance Date: 11/6/2000
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: Maize-chromosome 9 radiation hybrids (M9RHs) were obtained by irradiating oat seed monosomic for maize-chromosome 9 and self-pollinating the surviving plants. The M9RHs included plants with apparently normal maize- chromosome 9 and plants with various maize-chromosome 9 rearrangements. Variation in the transmission of these rearrangements might be expected. Therefore, we analyzed 580 progenies of 31 different M9RHs to evaluate the transmission of maize-chromosome 9 rearrangements. Apparently normal or deleted versions of maize-chromosome 9 were transmitted at frequencies of 20% and 11%, respectively. Intergenomic translocations transmitted at a higher frequency of 46%. In situ hybridization and molecular marker analyses indicated that complex rearrangements involving multiple deletions or translocations with interstitial deletions were transmitted in bloc. Lines that were homozygous for a given maize-chromosome 9 rearrangement were also identified. These homozygous lines are expected to stably transmit a rearrangement in subsequent generations. Sub-chromosome fragment stocks for maize-chromosome 9 are useful substrates for DNA-based marker mapping and for the manipulation of specific regions of maize-chromosome 9.