|RIERA LIZARAZU, O|
Submitted to: American Society of Agronomy Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 11/6/2000
Publication Date: N/A
Technical Abstract: By crossing maize cv. Seneca 60 to hexaploid oats, we produced 39 monosomic and disomic oat-maize addition lines. All ten maize chromosomes were retained as single additions to the haploid oat complement in 1.9% of more then 2000 vigorous F1 plants. A further 1.1% of the F1 plants possessed multiple maize chromosome additions. The added maize chromosomes were identified by FISH to root-tips and by PCR using chromosome-specific SSRs on leaf DNA. Meiotic restitution enhanced frequent development of partially and entirely unreduced gametes. Self-pollination of these semi-fertile F1 plants resulted in disomic additions for the maize chromosomes 1, 2, 3, 4, 6, 7, and 9. Maize chromosome 8 was recovered as a monosomic addition. Disomic additions for maize chromosomes 5 and 10 are under development by self-pollinating the appropriate F1 plants after colchicine-induced chromosome doubling. Here we report on the production technology and the morphological and genetic characterization of the first complete series of oat-maize chromosome addition lines. Allocation to chromosome of a set of previously unmapped DNA sequences and their use as a source of radiation hybrid lines demonstrate the value of oat-maize addition lines as a novel maize genomics tool. This work is supported by the National Science Foundation under Grant No. 9872650.