|Samac, Deborah - Debby|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/26/2000
Publication Date: N/A
Technical Abstract: Medicago truncatula (barrel medic) is becoming an important model species in dissecting molecular mechanisms of plant-microbe interactions associated with symbiotic nitrogen fixation, plant-mycorrhizal interactions, and plant disease resistance in legumes. Five plant cDNA libraries representing various aspects of plant-microbe interactions have been constructed from mRNA isolated from nitrogen-fixing root nodules (N), senescent nodules (SN), leaves and cotyledons (LC), leaves infected with Colletotrichum trifolii, and roots infected with Phytophthora medicaginis. A set of ESTs associated with nodule formation, pathogenesis-related and disease-related responses was identified by 5' end sequence analysis of approximately 250 random clones per library. A number of ESTs representing hormone- or stress-regulated genes, or genes associated with signal transduction and apoptosis was also identified. Their possible roles in plant-microbe interaction processes has been evaluated in micro- and macroarray experiments. Further progress in sequencing the cDNA libraries, which is currently being conducted at TIGR (The Institute for Genomic Research, Rockville, MD, USA), will become a platform for identification and functional analysis of the novel genes associated with plant-microbe interaction. The EST sequence data are available from GenBank and the M. truncatula database http://chrysie.tamu.edu/medicago.