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United States Department of Agriculture

Agricultural Research Service


item Zhang, Jianzhong
item Oleson, Arland
item Vacek, Don
item Ciomperlik, Matthew
item Gould, Juli
item Heilmann, Larry
item Nelson, Dennis

Submitted to: National Research and Action Plan for Silver Leaf Whitefly
Publication Type: Abstract Only
Publication Acceptance Date: 12/21/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: APHIS studies effectiveness of exotic species of Eretmocerus from Pakistan, Spain, United Arab Emirates & Ethiopia, to augment limited parasitism by native U.S. Eretmocerus. Assessment of these studies requires identification of parasitizing Eretmocerus. Small size & similar morphology make identification of adults difficult & early-stage larvae within the whitefly nearly impossible to detect & distinguish. We characterized satellite DNA sequences from 2 foreign species, E. mundus (Spain) & E. hyati (Pakistan), & one native U.S. species, E. eremicus. Also developed a DNA probe for the greenhouse biocontrol agent, Encarsia formosa. Each characterized sequence comprised 1-3% of the genome. Lengths of repeat units in each sequence: E. mundus (#3), 172 bp; E. mundus (#7), 348 bp; E. hyati, 251 bp; E. eremicus, 144 bp; & E. formosa, 33 bp. DNA probes from E. eremicus & E. formosa are specific and the probe from E. hyati is nearly specific (3% cross reaction with E. emiratus). E. mundus probe hybridizes with genome of all foreign species tested, but not with E. eremicus or E. tejanus; thus, it is useful for differentiating between foreign & native U.S. species of Eretmocerus. DNA probes were successfully evaluated for detection of parasitism in whitefly nymphs from cotton fields where Eretmocerus sp. (Ethiopia) had been released.

Last Modified: 07/20/2017
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