Submitted to: Annual Meeting Central States Entomological Society
Publication Type: Abstract Only
Publication Acceptance Date: 8/13/1999
Publication Date: N/A
Technical Abstract: Genetic variability in populations of northern corn rootworm. Annual Meeting, Kansas (Central States) Entomological Society, Manhattan, KS. We have begun to examine genetic variation of the nuclear rDNA ITS1 region in local and dispersed geographical populations of northern corn rootworm. Populations from Illinois, Iowa, South Dakota, Nebraska and Kansas were examined. Sequencing of the 644-646 bp amplicon revealed four polymorphic sites. PCR-RFLP analysis with the restriction enzyme Bcl I, detected two haplotypes. Interestingly, many beetles had both Bcl I haplotypes present, indicative of hetergeneity. The Illinois populations were fixed for haplotype "B", while South Dakota, Nebraska, and Kansas populations were predominately haplotype "A" or "A/B". A phylogeographic pattern of genetic diversity appears to be emerging.