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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Research Project #445653

Research Project: Development of Ethyl Formate and Irradiation Combination Treatment and its Application on Spotted Wing Drosophila and Mealybugs

Location: Tropical Crop and Commodity Protection Research

Project Number: 2040-43000-018-040-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Mar 1, 2024
End Date: Nov 30, 2026

Develop phytosanitary guidelines for the combination treatment of ethyl formate fumigation and irradiation to disinfest spotted wing drosophila and mealybugs in grape.

• Rear spotted wing drosophila Drosophila suzukii on artificial diet and determine life cycle period in colony. • Rear citrus mealybug (CMB) as a resistant surrogate of Pseudococcus comstki, grape mealybug most common in Campbell grape in Korea. • Determine efficacy of stand-alone EF treatment on different life stages of SWD and CMB using small scale fumigation chambers (0.03 m3). • Determine efficacy of stand-alone irradiation treatment on different life stages of SWD and CMB. • Conduct EF + irradiation combination treatment efficacy trials on SWD and CMB using LCt50 (for EF) and LD50 (for irradiation with either 30 Gy or 50 Gy) for respective EF- and radio-tolerant life stages. • Conduct combination of EF and irradiation treatment in different sequence, either irradiation treatment followed by EF treatment, or EF treatment followed by irradiation treatment. • Evaluate the effects of following treatments on grape quality (color, weight, sugar content, shelf-life, flavor) from (1) low-dose EF + irradiation treatment, (2) stand-alone EF treatment at LCt99%, and (3) stand-alone irradiation treatment at LD99% level. • Determine EF sorption in grape. • Determine EF concentration using a micro portable gas-chromatography with eTCD detector. • Determine irradiation dose using optichromic and alanine dosimeters. For irradiation treatment, a commercial 5 meV x-ray irradiator and a 165 kV Radsource research-scale x-ray irradiator are available.