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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Research Project #442470

Research Project: Hemp Genetics, Germplasm, and Biotechnology Collaboration: Phase I

Location: Vegetable Crops Research

Project Number: 5090-21000-070-001-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 1, 2022
End Date: Jun 30, 2027

- Establish genomic resources to enable effective association mapping studies in hemp. - Discover DNA markers and flower, grain, fiber characteristics that maximize industry profitability and match consumer preferences in hemp. - Deliver molecular and genetic resources to improve hemp yield and quality traits that maximize industry profitability and match consumer preferences. - Develop infrastructure for a hemp breeding program. - Develop, optimize, and implement genotype-flexible transformation and gene-editing methods. - Produce novel genetic resources using new breeding technologies to support discover and translational research.

Develop mapping and breeding populations for major gene pools. - Sequence mapping population parents and progeny and develop Single Nucleotide Polymorphisms (SNP) resources and a SNP genotyping platform. - Phenotype and genotype major quality/yield traits in mapping populations and identify SNPs and genes of interest. - Update greenhouse facilities and purchase hemp specific field equipment. - A baseline hemp transformation protocol will be optimized and efficiency improved by modifying media and related processes, integrating mechanical procedures, and evaluating priority cultivars. - Priority genes will be introduced into hemp and/or targeted by editing to support experimental and applied outcomes. Traits of interest will include secondary metabolites, plant development and productivity, and agronomic support and protection attributes.