Research Project: Rapid Antemortem Tests for the Early Detection of Transmissible Spongiform Encephalopathies and Other Animal Diseases

Location: Produce Safety and Microbiology Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Use of deep learning to predict chronic wasting disease status based on animal movement - (Peer Reviewed Journal)
Blaha, R.L., Silva, C.J., Cunningham, S.A., DeVivo, M.T., Edmunds, D.R., Boudreau, M.R. 2026. Use of deep learning to predict chronic wasting disease status based on animal movement. Movement Ecology. 14. Article 57. https://doi.org/10.1186/s40462-026-00668-4.

Mapping the surface of a prion using mass spectrometry - (Other)
Silva, C.J. 2026. Mapping the surface of a prion using mass spectrometry. Journal of American Society for Mass Spectrometry. 37(7) Cover.

VP2-targeted sandwich ELISA (sELISA) enables direct detection of Senecavirus A (SVA) - (Peer Reviewed Journal)
Hnasko, R.M., Lin, A.V., McGarvey, J.A. 2026. VP2-targeted sandwich ELISA (sELISA) enables direct detection of Senecavirus A (SVA). Journal of Virology. 100(6). Article e00571-26. https://doi.org/10.1128/jvi.00571-26.

Mass spectrometry-based approaches to mapping the surfaces of the infectious protein (prion) conformations of chronic wasting disease (CWD) and scrapie - (Abstract Only)
Silva, C.J. 2026. Mass spectrometry-based approaches to mapping the surfaces of the infectious protein (prion) conformations of chronic wasting disease (CWD) and scrapie. Chemistry Department Seminar, March 10, 2026. University at Albany, State University of New York. Meeting Abstract. 2026.

Quantifying oxidized methionines with mass spectrometry to map the surface of hamster prion strains Sc237 and 139H - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L. 2026. Quantifying oxidized methionines with mass spectrometry to map the surface of hamster prion strains Sc237 and 139H. Journal of American Society for Mass Spectrometry. 37(7):1527-1535. https://doi.org/10.1021/jasms.5c00355.

Aptamer-linked immobilized sorbent assay for detection of VP1 of foot and mouth disease virus serotype O - (Peer Reviewed Journal)
Quintela, I.A., Lopez-Magana II, R., Hwang, A., Vasse, T., Wu, V.C. 2026. Aptamer-linked immobilized sorbent assay for detection of VP1 of foot and mouth disease virus serotype O. Scientific Reports. 16. Article 5453. https://doi.org/10.1038/s41598-025-34793-8.

Recent advances in lateral flow devices and point-of-care diagnostics for highly pathogenic avian influenza A viruses - (Review Article)
Lin, M., Quintela, I.A., Sablani, S.S., Lin, C., Wu, V.C. 2025. Recent advances in lateral flow devices and point-of-care diagnostics for highly pathogenic avian influenza A viruses. Journal of Virology. 99(11). Article e01484-25. https://doi.org/10.1128/jvi.01484-25.

Protective effects of microalgal macular pigment on diabetic retinopathy upon blue light irradiation induced oxidative stress, inflammation, and MAPK pathways - (Peer Reviewed Journal)
Lin, M., Lin, C., Chiu, W., Chang, H., Cheang, W., Chang, C., Wu, V.C., Lin, C., Kuo, C. 2025. Protective effects of microalgal macular pigment on diabetic retinopathy upon blue light irradiation induced oxidative stress, inflammation, and MAPK pathways. Food Research International. 219. Article 116978. https://doi.org/10.1016/j.foodres.2025.116978.

Mass spectrometry-based sequencing of prion protein conformations - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Requena, J.R. 2025. Mass spectrometry-based sequencing of prion protein conformations. American Chemical Society Abstracts. ACS Fall 2025/Abstract #4309757. https://doi.org/10.1021/scimeetings.5c11363.

Mass spectrometry-based study of chronic wasting disease (CWD) prions from experimentally infected elk - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Cassmann, E.D., Greenlee, J.J. 2025. Mass spectrometry-based study of chronic wasting disease (CWD) prions from experimentally infected elk. American Chemical Society Abstracts. ACS Fall 2025/Abstract #4316725. https://doi.org/10.1021/scimeetings.5c11362.

Using nano-luciferase binary (NanoBiT) technology to assess the interaction between viral spike protein and angiotensin-converting enzyme II by aptamers - (Peer Reviewed Journal)
Lin, M., Lin, C., Chiang, H., Quintela, I.A., Wu, V.C., Lin, C. 2025. Using nano-luciferase binary (NanoBiT) technology to assess the interaction between viral spike protein and angiotensin-converting enzyme II by aptamers. BioTech. 14(1). Article 20. https://doi.org/10.3390/biotech14010020.

Elucidating the molecular docking and binding dynamics of aptamers with spike proteins across SARS-CoV-2 variants of concern - (Abstract Only)

Elucidating the molecular docking and binding dynamics of aptamers with spike proteins across SARS-CoV-2 variants of concern - (Peer Reviewed Journal)
Quintela, I.A., Vasse, T., Jian, D., Harrington, C., Sien, W., Wu, V.C. 2025. Elucidating the molecular docking and binding dynamics of aptamers with spike proteins across SARS-CoV-2 variants of concern. Frontiers in Microbiology. 16. Article 1503890. https://doi.org/10.3389/fmicb.2025.1503890.

Comparing the extent of methionine oxidation in the prion and native conformations of PrP - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L., Requena, J.R. 2025. Comparing the extent of methionine oxidation in the prion and native conformations of PrP. ACS Omega. 10(1):1320-1330. https://doi.org/10.1021/acsomega.4c08892.

Quantifying the molecular properties of the elk chronic wasting disease agent with mass spectrometry - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L., Cassmann, E.D., Greenlee, J.J. 2024. Quantifying the molecular properties of the elk chronic wasting disease agent with mass spectrometry. Pathogens. 13(11). Article 1008. https://doi.org/10.3390/pathogens13111008.

Using a synthetic gene to generate stable isotope labeled internal standard peptides for quantitation of Shiga toxins - (Abstract Only)
C. J. Silva, M. L. Erickson-Beltran, B. G. Lee, and B. Quiñones. 2024. Using a synthetic gene to generate stable isotope labeled internal standard peptides for quantitation of Shiga toxins. Abstracts of Papers of the American Chemical Society. Fall 2024 (Denver, Colorado, August 21, 2024). Division of Analytical Chemistry abstract # 4101573.

Aptamer-linked Immobilized Sorbent Assay for detecting Foot and Mouth Disease (FMD) Virus VP1 Serotype O - (Abstract Only)

Characterizing chronic wasting disease (CWD) prevalence through remote sensing - (Abstract Only)
Moody, J., Iglay, R., Silva, C.J., Tomar, S. 2024. Characterizing Chronic Wasting Disease (CWD) prevalence through remote sensing. Mississippi State University’s 2024 Undergraduate Research Scholars Symposium (April 11-12, 2024). Mississippi State University, USA. Poster # 243. Page 96 (OPES Infographic.pdf (8.5 x 11 in))

A mass spectrometry-based method of quantifying the contribution of the lysine polymorphism at position 171 in sheep PrP - (Peer Reviewed Journal)
Silva, C.J., Cassmann, E.D., Greenlee, J.J., Erickson-Beltran, M.L., Requena, J.R. 2023. A mass spectrometry-based method of quantifying the contribution of the lysine polymorphism at position 171 in sheep PrP. Journal of American Society for Mass Spectrometry. 34(2):245-254. https://doi.org/10.1021/jasms.2c00277.

General method of quantifying the extent of methionine oxidation in the prion protein - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L. 2023. General method of quantifying the extent of methionine oxidation in the prion protein. Journal of American Society for Mass Spectrometry. 34(2):255-263. https://doi.org/10.1021/jasms.2c00280.

Mass spectrometry-based quantitation of methionine oxidation to assess prion structures - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L. 2022. Mass spectrometry-based quantitation of methionine oxidation to assess prion structures. 10th Iberian Congress on Prions, Vila Real Portugal. May 19-20, 2022. Book Abstract. May 20, 2022. Page 41.

Chronic wasting disease (CWD), an evolving prion disease - (Abstract Only)
Silva, C.J. 2022. Chronic wasting disease (CWD), an evolving prion disease. Meeting Abstract. Speaker 10. Page 1. https://events-prod2.stanford.edu/events/942/94241/.