Research Project: Intervention Strategies to Control and Eradicate Foreign Animal Diseases of Swine

Location: Foreign Animal Disease Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

ASF vaccine candidate ASFV-G-deltaI177l does not exhibit residual virulence in long-term clinical studies - (Peer Reviewed Journal)
Borca, M.V., Ramirez Medina, E., Silva, E.B., Ayushi, R., Espinoza, N.N., Velazquez Salinas, L., Gladue, D.P. 2023. ASF vaccine candidate ASFV-G-deltaI177l does not exhibit residual virulence in long-term clinical studies. Pathogens. 12(6). https://doi.org/10.3390/pathogens12060805.

Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines - (Peer Reviewed Journal)
Velazquez Salinas, L., Ramirez Medina, E., Rai, A., Pruitt, S.E., Vuono, E., Espinoza, N.N., Gay, C.G., Witte, S.B., Gladue, D.P., Borca, M.V. 2023. Confirming the absence of parental African swine fever virus as a potential contaminant of recombinant live attenuated ASF vaccines. Biologicals. 3;83:101685. https://doi.org/10.1016/j.biologicals.2023.101685.

Pathogenesis and transmissibility studies of the ASFV strain currently causing outbreaks in the Dominican Republic - (Abstract Only)
Ramirez Medina, E., O'Donell, V., Silva, E.B., Espinoza, N.N., Velazquez Salinas, L., Moran, K., Daite, D., Barrette, R., Bonto, F., Holland, R., Gladue, D.P., Borca, M.V. 2023. Pathogenesis and transmissibility studies of the ASFV strain currently causing outbreaks in the Dominican Republic. Meeting Abstract. https://doi.org/10.3390/v14051090.

Classical swine fever virus structural glycoprotein E2 interacts with host protein ACADM during the virus infectious cycle - (Peer Reviewed Journal)
Vuono, E., Ramirez Medina, E., Silva, E.B., Berggren, K., Rai, A., Espinoza, N.N., Gladue, D.P., Borca, M.V. 2023. Classical swine fever virus structural glycoprotein E2 interacts with host protein ACADM during the virus infectious cycle. Viruses. 15(5). https://doi.org/10.3390/v15051036.

Characterization of a novel African swine fever virus p72 genotype II from Nigeria - (Peer Reviewed Journal)
Ambagala, A., Goonewardene, K., Lamboo, L., Goolia, M., Erdelyan, C., Fisher, M., Handel, K., Lung, O., Blome, S., King, J., Forth, J.H., Calvelage, S., Spinard Iii, E.J., Gladue, D.P., Masembe, C., Adedeji, A.J., Olubade, T., Maurice, N.A., Ularamu, H.G., Luka, P.D. 2023. Characterization of a novel African swine fever virus p72 genotype II from Nigeria. Viruses. https://doi.org/10.3390/v15040915.

Development of porcine monoclonal antibodies with neutralizing activity against classical swine fever virus from C-strain E2 specific single B cells - (Peer Reviewed Journal)
Wang, L., Madera, R., Li, Y., Gladue, D.P., Borca, M.V., McIntosh, M., Shi, J. 2023. Development of porcine monoclonal antibodies with neutralizing activity against classical swine fever virus from C-strain E2 specific single B cells. Viruses. 15(4);863. https://doi.org/10.3390/v15040863.

Evaluation of potential recombination events in codon deoptimized FMDV strains - (Abstract Only)
Spinard, E., Fish, I., Azzinaro, P.A., Hartwig, E.J., Smoliga, G.R., Mogulothu, A., Rodriguez-Calzada, M., Arzt, J., De Los Santos, T.B., Medina, G.N. 2023. Evaluation of potential recombination events in codon deoptimized FMDV strains. Meeting Abstract. https://doi.org/10.3390/v15030670.

Evaluation of the function of ASFV gene E66L in the process of virus replication and virulence in swine - (Peer Reviewed Journal)

Editorial: Insights of important mammalian viruses: Infection, pathogenesis and drugs - (Peer Reviewed Journal)
Li, Y., Gladue, D.P., Li, S., Deng, L., Shen, C., Aguilar, P., Wang, T., Kong, L. 2023. Editorial: Insights of important mammalian viruses: Infection, pathogenesis and drugs. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2023.1147109.

Whole genome sequencing and phylogenetic analysis of African swine fever virus detected from a backyard pig in Mongolia, 2019 - (Peer Reviewed Journal)
Hyeon, J., Tseren-Ochir, E., Lee, D., Gladue, D.P., Borca, M.V., Risatti, G.R. 2023. Whole genome sequencing and phylogenetic analysis of African swine fever virus detected from a backyard pig in Mongolia, 2019. Viruses. 10. https://doi.org/10.3389/fvets.2023.1094052.

Full genome sequence for the African swine fever virus outbreak in the Dominican Republic in 1980 - (Peer Reviewed Journal)
Spinnard, E., O'Donnell, V., Vuono, E., Davis, C., Rai, A., Ramirez Medina, E., Espinoza, N.N., Valadares, A., Borca, M.V., Gladue, D.P. 2023. Full genome sequence for the African swine fever virus outbreak in the Dominican Republic in 1980. Microbiology Resource Announcements. 13:1024(2023). https://doi.org/10.1038/s41598-022-25987-5.

A highly effective African swine fever virus vaccine elicits a memory T cell response in vaccinated swine - (Peer Reviewed Journal)
Attreed, S.E., Silva, C.M., Abbott, S.T., Ramirez Medina, E., Espinoza, N.N., Borca, M.V., Gladue, D.P., Diaz San Segundo, F.C. 2022. A highly effective African swine fever virus vaccine elicits a memory T cell response in vaccinated swine. Pathogens. 11(12). Article 1438. https://doi.org/10.3390/pathogens11121438.

Deletion of an African swine fever ATP-dependent RNA hel-icase QP509L from the highly virulent Georgia 2010 strain does not affect replication or virulence - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Pruitt, S., Rai, A., Espinoza, N.N., Spinard III, E.J., Valadares, A., Silva, E.B., Velazquez Salinas, L., Borca, M.V., Gladue, D.P. 2022. Deletion of an African swine fever ATP-dependent RNA hel-icase QP509L from the highly virulent Georgia 2010 strain does not affect replication or virulence. Viruses. 14(11). https://doi.org/10.3390/v14112548.

The presence of virus neutralizing antibodies is highly associated with protection against virulent challenge in domestic pigs immunized with ASFV live attenuated vaccine candidates - (Peer Reviewed Journal)
Silva, E.B., Krug, P., Ramirez Medina, E., Pruitt, S., Rai, A., Espinoza, N.N., Valadares, A., Gladue, D.P., Borca, M.V. 2022. The presence of virus neutralizing antibodies is highly associated with protection against virulent challenge in domestic pigs immunized with ASFV live attenuated vaccine candidates. Microbiology Resource Announcements. 11(11):1311. https://doi.org/10.3390/pathogens11111311.

Structural predictions of the complete ASFV-G proteome - (Peer Reviewed Journal)
Spinard III, E.J., Azzinaro, P.A., Rai, A., Espinoza, N.N., Ramirez Medina, E., Borca, M.V., Gladue, D.P. 2022. Structural predictions of the complete ASFV-G proteome. Microbiology Resource Announcements. 11(12):e0088122. https://doi.org/10.1128/mra.00881-22.

Mutation of FMDV Lpro residues outside substrate binding domain drives viral attenuation - (Abstract Only)
Diaz San Segundo, F.C., Azzinaro, P.A., Zhu, J.J., Medina, G.N., De Los Santos, T.B. 2022. Mutation of FMDV Lpro residues outside substrate binding domain drives viral attenuation. Meeting Abstract. https://doi.org/10.3389/fvets.2022.1028077.

African Swine Fever Vaccinology: Why Is It Difficult? - (Peer Reviewed Journal)
Zhu, J.J. 2022. African Swine Fever Vaccinology: Why Is It Difficult? . Viruses. https://doi.org/10.3390/v14092021.

African Swine Fever Vaccinology: Why Is It Difficult? - (Peer Reviewed Journal)
Zhu, J.J. 2022. African Swine Fever Vaccinology: Why Is It Difficult?. Viruses. https://doi.org/10.3390/v14092021.

The FlagT4G vaccine confers a strong and regulated innate immunity that correlates with early virological protection against Classical swine fever - (Peer Reviewed Journal)
Bohórquez, J.A., Wang, M., Diaz, I., Alberch, M., Perez-Simo, M., Rosell, R., Gladue, D.P., Borca, M.V., Llilianne, G. 2022. The FlagT4G vaccine confers a strong and regulated innate immunity that correlates with early virological protection against classical swine fever. Viruses. 14(9). https://doi.org/10.3390/v14091954.

ASFV Gene A151R is involved in the process of virulence in domestic swine - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Pruitt, S., Rai, A., Espinoza, N.N., Valladres, A., Spinard Iii, E.J., Silva, E.B., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. ASFV Gene A151R is involved in the process of virulence in domestic swine. Viruses. 14(8):1834. https://doi.org/10.3390/v14081834.

Deletion of the EP296R gene from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs - (Abstract Only)
Vuono, E., Ramirez Medina, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Spinard Iii, E.J., Valladares, A., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Deletion of the EP296R gene from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs. Virus Research. https://doi.org/10.3390/v14081682.

Deletion of the ASFV dUTPase gene E165R from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs - (Peer Reviewed Journal)
Vuono, E., Ramirez Medina, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Silva, E.B., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2022. Deletion of the ASFV dUTPase gene E165R from the genome of highly virulent African swine fever virus Georgia 2010 does not affect virus replication or virulence in domestic pigs. Viruses. 14(7):1409. https://doi.org/10.3390/v14071409.

Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry - (Peer Reviewed Journal)
Canter, J.A., Aponte, T., Ramirez Medina, E., Pruitt, S.E., Neilan, J., Gladue, D.P., Borca, M.V., Zhu, J.J. 2022. Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry. Scientific Reports. https://doi.org/10.3390/v14061249.

Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometryg - (Abstract Only)
Canter, J.A., Aponte, T., Ramirez Medina, E., Pruitt, S.E., Gladue, D.P., Borca, M.V., Zhu, J.J. 2022. Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometryg. Meeting Abstract. https://doi.org/10.3390/v15040915.

Recombinant ASF live attenuated virus strains as experimental vaccine candidates - (Peer Reviewed Journal)
Gladue, D.P., Borca, M.V. 2022. Recombinant ASF live attenuated virus strains as experimental vaccine candidates. Viruses. https://doi.org/10.3390/v14050878.

Evaluation of the safety profile of the ASFV vaccine candidate ASFV-G-I177L - (Peer Reviewed Journal)
Xuan, T., Le Thi Thu, P., Nguyen Quang, H., Do Thanh, T., Nguyen Van, D., Pham Hào, Q., Quách Võ, N., Gay, C.G., Gladue, D.P., Borca, M.V. 2022. Evaluation of the safety profile of the ASFV vaccine candidate ASFV-G-I177L. Viruses. https://doi.org/10.3390/v14050896.

Identification of genetic deletions in ASFV that could be potentially used as targets for the development of DIVA vaccines - (Abstract Only)
Vuono, E., Ramirez Medina, E., Silva, E.B., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez Salinas, L., Borca, M.V., Gladue, D.P. 2022. Identification of genetic deletions in ASFV that could be potentially used as targets for the development of DIVA vaccines. Meeting Abstract. https://doi.org/10.1128/JVI.01419-21.

Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez Salinas, L., Pina-Pedrero, S., Zhu, J.J., Rodriguez, F., Borca, M.V., Gladue, D.P. 2021. Deletion of E184L, a putative DIVA target from the pandemic strain of African Swine Fever Virus, produces a reduction in virulence and protection against virulent challenge. Viruses. https://doi.org/10.1128/JVI.01419-21.

Pegylated Interferon vs Standard Interferon: a promising biotherapeutic platform to get strong and long-lasting antiviral activity against FMDV - (Proceedings)
Diaz San Segundo, F.C., Medina, G.N., De Los Santos, T.B. 2021. Pegylated Interferon vs Standard Interferon: a promising biotherapeutic platform to get strong and long-lasting antiviral activity against FMDV. Global Foot-and Mouth Disease Research Alliance (GFRA). https://doi.org//10.3389/fmicb.2021.668890.

Evaluation of the deletion of ASFV MGF110-5L-6L on swine virulence and its potential use as a DIVA vaccine marker gene - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Pruitt, S.E., Rai, A., Valladares, A., Espinoza, N.N., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2021. Evaluation of the deletion of ASFV MGF110-5L-6L on swine virulence and its potential use as a DIVA vaccine marker gene. Viruses. https://doi.org/10.3390/v13020286.