Location: Sugarbeet and Potato Research
Project Number: 3060-21430-009-006-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 1, 2021
End Date: Jul 31, 2025
Objective:
Utilizing integrated omics approaches to have a better understanding of molecular, biochemical, and physiological mechanisms regulating wound-healing in potato.
Approach:
After mechanical wounding, transcriptome, metabolome and phytohormone profiles will be obtained to discover the underlying molecular mechanisms that control wound-healing processes. The cooperator will carry out the following: 1) Induce mechanical wounding by excising parenchyma discs (15 mm diameter × 3 mm thick) in tubers and allow the tissues to heal according to the established tuber wound-healing model systems for potato. 2) Determine wound-healing progression histochemically by suberization ratings in blocks of tissue from tuber discs at different time points (0, 0.5, 1, 3, 5, 7, 9 days post wounding). 3) Collect tissue samples at aforementioned time points using a subset of tubers for transcriptome, phytohormone and metabolome analyses. 4) Transcriptome profiling. RNA will be extracted from each sample using a commercial kit, and cDNA library preparation and RNA sequencing will be performed by a service laboratory. 5) Metabolome profiling. Monoterpenoid volatiles, polar amino acids, or hydrophobic lipids profiles will be assessed using common LC/MS untargeted metabolic profiling methods. 6) Phytohormone profiling. Auxin, abscisic acid, cytokinins, gibberellic acid, salicylic acid, and jasmonic acid will be determined using a service laboratory. 7) Analyze transcriptome, phytohormone, and metabolome data to identify regulatory genes, gene-sets, pathways, metabolites, and phytohormones. 8) Interpret results to explain molecular mechanisms controlling wound healing processes in a final report.
