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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Research Project #439334

Research Project: CucCAP 2: Harnessing Genomic Resources for Disease Resistance and Management in Cucurbit Crops – Bringing the Tools to the Field

Location: Vegetable Crops Research

Project Number: 5090-21000-069-056-A
Project Type: Cooperative Agreement

Start Date: Sep 1, 2020
End Date: Jul 31, 2024

Objective:
1. Develop and characterize cucumber core population (390 cucumber lines). 2. Molecular mapping of downy mildew (DM) resistances in cucumber inbred line PT108. 3. Marker-assisted pyramiding of DM resistance QTL and evaluation of enhanced germplasm with multiple disease resistances.

Approach:
Objective 1: 1) Re-sequencing and analyze the data for 384 cucumber lines. 2) Phenotype for target traits in this collection for downy mildew resistance. 3) Conduct association analysis for downy mildew resistance. Objective 2: 1) Fine mapping dm4.1, dm5.2, and dm5.3 major-effect QTL. 2) QTL mapping of DM resistance in PT108 from exotic source. Objective 3: 3). Marker-assisted QTL pyramiding of dm4.1, dm5.2 and dm5.3 into elite pickling cucumber background Gy14. Evaluate its performance in field and growth chambers.