Research Project: Improving Nutrient Utilization to Increase the Production Efficiency and Sustainability of Rainbow Trout Aquaculture

Location: Small Grains and Potato Germplasm Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Evaluating inclusion of commercial pistachio by-product as functional ingredients in rainbow trout fishmeal and plant-meal based diets - (Peer Reviewed Journal)
Abanikannda, M.F., Shiflett, M., Morais, A., Hong, J., Sealey, W.M., Bledsoe, J.W. 2024. Evaluating inclusion of commercial pistachio by-product as functional ingredients in rainbow trout fishmeal and plant-meal based diets. Antioxidants. 13(11), 1280. https://doi.org/10.3390/antiox13111280.

Optimizing growth and mitochondrial function in rainbow trout, Oncorhynchus mykiss through eco-friendly dietary and changes in water temperature regimen strategies - (Peer Reviewed Journal)
Idenyi, J.N., Abdallah, H., Adeyemi, A.D., Huber, D.H., Gannam, A., Sealey, W.M., Igwe, D.O., Eya, J.C. 2024. Optimizing growth and mitochondrial function in rainbow trout, Oncorhynchus mykiss through eco-friendly dietary and changes in water temperature regimen strategies. Aquaculture. 595:741591. https://doi.org/10.1016/j.aquaculture.2024.741591.

Determination of the protein value of novel poultry meal ingredients for rainbow trout (Oncorhynchus mykiss) - (Peer Reviewed Journal)

Impacts of alfalfa nutrient concentration on pellet physical attribution, growth performance, metabolism and nutritional quality of rainbow trout, Oncorhynchus mykiss - (Peer Reviewed Journal)

New methodology to determine mass composition of protease inhibitors (Kunitz trypsin inhibitor and Bowman-Birk inhibitor) in soybeans - (Abstract Only)
Liu, K. 2024. New methodology to determine mass composition of protease inhibitors (Kunitz trypsin inhibitor and Bowman-Birk inhibitor) in soybeans. Annual Meeting and Expo of the American Oil Chemists' Society. Available. https://annualmeeting.aocs.org/program/technical-program.

Investigation of the suitability of 3 insect meals as protein sources for rainbow trout (Oncorhynchus mykiss) - (Peer Reviewed Journal)
Owens, C.E., Powell, M.S., Gaylord, T., Conley, Z.B., Sealey, W.M. 2024. Investigation of the suitability of 3 insect meals as protein sources for rainbow trout (Oncorhynchus mykiss). Journal of Economic Entomology. 117(4):1254-1260. https://doi.org/10.1093/jee/toae037.

Integrated alternative approaches to select feed-efficient rainbow trout families to enhance the plant protein utilization - (Peer Reviewed Journal)
Kajbaf, K., Overturf, K.E., Kumar, V. 2024. Integrated alternative approaches to select feed-efficient rainbow trout families to enhance the plant protein utilization. Scientific Reports. 14. Article 3869. https://doi.org/10.1038/s41598-024-54218-2.

An international collaborative study on the new American Oil Chemists' Society (AOCS) proposed method Ba 15-2023 for measuring protein solubility index - (Abstract Only)
Liu, K., West, T., Wu, X., Liu, F. 2024. An international collaborative study on the new American Oil Chemists' Society (AOCS) proposed method Ba 15-2023 for measuring protein solubility index. Annual Meeting and Expo of the American Oil Chemists' Society. Available. https://annualmeeting.aocs.org/program/technical-program.

Antinutritional factors in protein-rich plant products: chemistry, occurrence, nutritional implications, and their analysis - (Book / Chapter)
Albe-Slabi, S., Liu, K., Amarowicz, R., Kapel, R., Pegg, R., Wanasundara, J. 2024. Antinutritional factors in protein-rich plant products: chemistry, occurrence, nutritional implications, and their analysis. Book Chapter. Available. https://shop.elsevier.com/books/functionality-of-plant-proteins/wanasundara/978-0-323-91721-6.

Enzymatic and algebraic methodology to determine the contents of Kunitz and Bowman-Birk inhibitors and their contributions to total trypsin or chymotrypsin inhibition in soybeans - (Peer Reviewed Journal)
Liu, K. 2024. Enzymatic and algebraic methodology to determine the contents of Kunitz and Bowman-Birk inhibitors and their contributions to total trypsin or chymotrypsin inhibition in soybeans. Journal of Agricultural and Food Chemistry. 72(20):11782-11793. https://doi.org/10.1021/acs.jafc.3c06389.

Effects of fishmeal substitution with mealworm meals (Tenebrio molitor and Alphitobius diaperinus) on the growth, physiobiochemical response, digesta microbiome, and immune genes expression of Atlantic salmon - (Peer Reviewed Journal)
Habte-Tsion, H.M., Hawkyard, M., Sealey, W.M., Bradshaw, D., Meesala, K., Bouchard, D. 2024. Effects of fishmeal substitution with mealworm meals (Tenebrio molitor and Alphitobius diaperinus) on the growth, physiobiochemical response, digesta microbiome, and immune genes expression of Atlantic salmon. Aquaculture Nutrition. 2024(1). Article 6618117. https://doi.org/10.1155/2024/6618117.

Effects of dietary arachidonic acid supplementation in low fishmeal and fish oil-free diets on growth performance, inflammatory response, gut histology, and non-specific immunity in sub-adult rainbow trout, Oncorhynchus mykis - (Peer Reviewed Journal)
Hong, J., Ortiz, J.G., Sealey, W.M., Small, B.C. 2023. Effects of dietary arachidonic acid supplementation in low fishmeal and fish oil-free diets on growth performance, inflammatory response, gut histology, and non-specific immunity in sub-adult rainbow trout, Oncorhynchus mykiss. Aquaculture. 580. Article 740272. https://doi.org/10.1016/j.aquaculture.2023.740272.

Digestibility of corn distiller’s dried grains with solubles (DDGS) in practical feeds with phytase and xylanase treatment fed to Nile Tilapia - (Peer Reviewed Journal)
Welker, T.L., Sealey, W.M., Tan, R. 2023. Digestibility of corn distiller’s dried grains with solubles (DDGS) in practical feeds with phytase and xylanase treatment fed to Nile Tilapia. North American Journal of Aquaculture. 86(1):154-162. https://doi.org/10.1002/naaq.10325.

Genome-wide insights into whole gut microbiota of rainbow trout, Oncorhynchus mykiss, fed plant proteins and camelina oil at different temperature regimens - (Peer Reviewed Journal)
Idenyi, J.N., Abanikannda, M., Huber, D.H., Gannam, A., Sealey, W.M., Eya, J.C. 2023. Genome-wide insights into whole gut microbiota of rainbow trout, Oncorhynchus mykiss, fed plant proteins and camelina oil at different temperature regimens. Journal of the World Aquaculture Society. 55(2). Article e13028. https://doi.org/10.1111/jwas.13028.

Controlling eutrophication of aquaculture production water using biochar: correlation of molecular composition with adsorption characteristics as revealed by FT-ICR mass spectrometry - (Peer Reviewed Journal)
Rance, B., Struhs, E., Mirkouei, A., Overturf, K.E., Chacon-Patino, M., Mckenna, A., Chen, H., Raja, K.S. 2023. Controlling eutrophication of aquaculture production water using biochar: correlation of molecular composition with adsorption characteristics as revealed by FT-ICR mass spectrometry. Processes. 11(10), 2883. https://doi.org/10.3390/pr11102883.

Improved fecal particle size profile in rainbow trout fed feeds containing different ratios of animal meal and plant protein concentrates: Effect on nitrogen and phosphorus partitioning - (Peer Reviewed Journal)
Welker, T.L., Barrows, F.T. 2023. Improved fecal particle size profile in rainbow trout fed feeds containing different ratios of animal meal and plant protein concentrates: Effect on nitrogen and phosphorus partitioning. North American Journal of Aquaculture. 86(1):84-94. https://doi.org/10.1002/naaq.10315.

Evidence of a divided nutritive function in the rainbow trout (Oncorhynchus mykiss) midgut and hindgut microbiomes by whole shotgun metagenomic approach - (Peer Reviewed Journal)
Betiku, O., Yeoman, C., Gaylord, T., Ishaq, S., Duff, G., Sealey, W.M. 2023. Evidence of a divided nutritive function in the rainbow trout (Oncorhynchus mykiss) midgut and hindgut microbiomes by whole shotgun metagenomic approach. Aquaculture Reports. 30. Article 101601. https://doi.org/10.1016/j.aqrep.2023.101601.

Novel and simple method to determine contents of Kunitz trypsin inhibitor and Bowman-Birk inhibitor in soybeans - (Abstract Only)
Liu, K. 2023. Novel and simple method to determine contents of Kunitz trypsin inhibitor and Bowman-Birk inhibitor in soybeans. Meeting Abstract. Day 1, B1 Session, 6/19/23. Available. https://www.wsrc11vienna.com/.

Engineered biomaterials for reducing phosphorus and nitrogen levels from downstream water of aquaculture facilities - (Peer Reviewed Journal)
Bare, W., Struhs, E., Mirkouei, A., Overturf, K.E., Small, B. 2023. Engineered biomaterials for reducing phosphorus and nitrogen levels from downstream water of aquaculture facilities. Nature Sustainability. 11(4). Article 1029. https://doi.org/10.3390/pr11041029.

Evaluation of a low-resource soy protein production method and its products - (Peer Reviewed Journal)
Gulkirpik, E., Donnelly, A., Nowakunda, K., Liu, K., Andrade Laborde, J.E. 2023. Evaluation of a low-resource soy protein production method and its products. Frontiers in Nutrition. 10. Article 1067621. https://doi.org/10.3389/fnut.2023.1067621.

Chymotrypsin inhibitor assay: Expressing, calculating, and standardizing inhibitory activity in absolute amounts of chymotrypsin inhibited - (Peer Reviewed Journal)
Liu, K. 2023. Chymotrypsin inhibitor assay: Expressing, calculating, and standardizing inhibitory activity in absolute amounts of chymotrypsin inhibited. Sustainable Food Proteins. 1(1):30-44. https://doi.org/10.1002/sfp2.1004.

Protein solubility index (PSI): a new simple and unified index for measuring plant protein quality - (Abstract Only)
Liu, K. 2023. Protein solubility index (PSI): a new simple and unified index for measuring plant protein quality. Annual Meeting and Expo of the American Oil Chemists' Society. Available. https://aocs.eventscribe.net/SearchByBucket.asp?f=TrackName&pfp=BrowsebyBucket.

Activities of trypsin and chymotrypsin inhibitors and their relationship in selected soybean germplasm - (Abstract Only)
Liu, K., Woolman, M.J. 2023. Activities of trypsin and chymotrypsin inhibitors and their relationship in selected soybean germplasm. Annual Meeting and Expo of the American Oil Chemists' Society. Available. https://aocs.eventscribe.net/SearchByBucket.asp?f=TrackName&pfp=BrowsebyBucket.

A new method for determining protein solubility index (PSI) based on extraction with 5 mM alkali hydroxide and its correlation with trypsin inhibitor activity in soybean products - (Peer Reviewed Journal)
Liu, K. 2022. A new method for determining protein solubility index (PSI) based on extraction with 5 mM alkali hydroxide and its correlation with trypsin inhibitor activity in soybean products. Journal of the American Oil Chemists' Society. 99(10):855–871. https://doi.org/10.1002/aocs.12643.

Chymotrypsin inhibitor assay: expressing and standardizing inhibitor activity in absolute amounts of chymotrypsin inhibited - (Abstract Only)
Liu, K., Woolman, M.J. 2022. Chymotrypsin inhibitor assay: expressing and standardizing inhibitor activity in absolute amounts of chymotrypsin inhibited. Meeting Abstract. Available: https://www.iufostworldcongress-singapore.com/agenda

Sorghum as a potential valuable aquafeed ingredient: Nutritional quality and digestibility - (Peer Reviewed Journal)
Zarei, M., Amirkolaie, A.K., Trushenski, J.T., Sealey, W.M., Schwarz, M.H., Ovissipour, R. 2022. Sorghum as a potential valuable aquafeed ingredient: Nutritional quality and digestibility. Agriculture. 12(5). Article 669. https://doi.org/10.3390/agriculture12050669.

Multi-tissue RNAseq reveals genetic and temporal differences in acute viral (IHN) infection among three selected lines of rainbow trout with varying resistance - (Peer Reviewed Journal)
Bledsoe, J.W., Ma, J., Cain, K., Bruce, T., Rawles, A.A., Abernathy, J.W., Welker, T.L., Overturf, K.E. 2022. Multi-tissue RNAseq reveals genetic and temporal differences in acute viral (IHN) infection among three selected lines of rainbow trout with varying resistance. Fish and Shellfish Immunology. 124:343-361. https://doi.org/10.1016/j.fsi.2022.03.034.

New and improved methods for measuring acid insoluble ash - (Peer Reviewed Journal)
Liu, K. 2022. New and improved methods for measuring acid insoluble ash. Animal Feed Science and Technology. 288. Article 115282. https://doi.org/10.1016/j.anifeedsci.2022.115282.

Method development and optimization for measuring chymotrypsin and chymotrypsin inhibitor activities - (Peer Reviewed Journal)
Liu, K. 2022. Method development and optimization for measuring chymotrypsin and chymotrypsin inhibitor activities. Journal of Food Science. 87(5):2018–2033. https://doi.org/10.1111/1750-3841.16141.

Improving and developing sustainable methods for plant protein processing - (Abstract Only)
Liu, K. 2022. Improving and developing sustainable methods for plant protein processing. Meeting Abstract for 2022 AOCS Annual Meeting & Expo, May 1-4, 2022 (hybrid). 1/2. https://22aocs.meetbreakout.com/on-demand/improving-and-developing-sustainable-methods-for-protein-p.

Developing an optimized method for measuring chymotrypsin inhibitor activity in protein products - (Abstract Only)
Liu, K., Woolman, M.J. 2022. Developing an optimized method for measuring chymotrypsin inhibitor activity in protein products. Meeting Abstract for 2022 AOCS Annual Meeting & Expo, May 1-4, 2022 (hybrid). 2/2. https://22aocs.meetbreakout.com/on-demand/developing-an-optimized-method-for-measuring-chymotrypsin-inhibitor-activity-in-protein-products

Simplified analysis and expanded profiles of avenanthramides in oat grains - (Peer Reviewed Journal)
Woolman, M.J., Liu, K. 2022. Simplified analysis and expanded profiles of avenanthramides in oat grains. Foods. 11(4). Article 560. https://doi.org/10.3390/foods11040560.

Latitude oil as a sustainable alternative to dietary fish oil in rainbow trout (Oncorhynchus mykiss): Effects on filet fatty acid profiles, intestinal histology, and plasma biochemistry - (Peer Reviewed Journal)
Hong, J., Bledsoe, J.W., Overturf, K.E., Lee, S., Iassonova, D., Small, B. 2022. Latitude oil as a sustainable alternative to dietary fish oil in rainbow trout (Oncorhynchus mykiss): Effects on filet fatty acid profiles, intestinal histology, and plasma biochemistry. Frontiers in Sustainable Food Systems. 6. Article 837628. https://doi.org/10.3389/fsufs.2022.837628.

Different dietary combinations of high/low starch and fat with or without bile acid supplementation on growth, liver histopathology, gene expression and fatty acid composition of largemouth bass, Micropterus salmoides - (Peer Reviewed Journal)
Romano, N., Fischer, H., Rubio-Benito, M., Overturf, K.E., Sinha, A., Kumar, V. 2022. Different dietary combinations of high/low starch and fat with or without bile acid supplementation on growth, liver histopathology, gene expression and fatty acid composition of largemouth bass, Micropterus salmoides. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology. 266. Article 111157. https://doi.org/10.1016/j.cbpa.2022.111157.

Evaluation of the ability of Pichia guilliermondii to improve growth performance and disease resistance in rainbow trout (Oncorhynchus mykiss) - (Peer Reviewed Journal)
Sealey, W.M., Conley, Z.B., Hinman, B.T., O'Neill, T.J., Bowzer, J., Block, S. 2022. Evaluation of the ability of Pichia guilliermondii to improve growth performance and disease resistance in rainbow trout (Oncorhynchus mykiss). Journal of the World Aquaculture Society. 53(2):411-423. https://doi.org/10.1111/jwas.12872.

Comparison of ISO 14902:2001 with AOCS Ba 12a-2020 for determining trypsin inhibitor activity in protein products - (Peer Reviewed Journal)
Liu, K. 2021. Comparison of ISO 14902:2001 with AOCS Ba 12a-2020 for determining trypsin inhibitor activity in protein products. Journal of the American Oil Chemists' Society. https://doi.org/10.1002/aocs.12542.

Digesta and plasma metabolomics of rainbow trout strains with varied yolerance of plant-based diets highlights potential for non-lethal assessments of enteritis development - (Peer Reviewed Journal)
Palma, M., Bledsoe, J.W., Tavares, L.C., Romano, N., Small, B., Viegas, I., Overturf, K.E. 2021. Digesta and plasma metabolomics of rainbow trout strains with varied yolerance of plant-based diets highlights potential for non-lethal assessments of enteritis development. Metabolites. 11(9). Article 11090590. Available: https://doi.org/10.3390/metabo11090590.

Insect (black soldier fly larvae) oil as a potential substitute for fish or soy oil in the fish meal-based diet of juvenile rainbow trout (Oncorhynchus mykiss) - (Peer Reviewed Journal)
Fawole, F.J., Labh, S.N., Hossain, M.S., Overturf, K.E., Small, B.C., Welker, T.L., Hardy, R.W., Kumar, V. 2021. Insect (black soldier fly larvae) oil as a potential substitute for fish or soy oil in the fish meal-based diet of juvenile rainbow trout (Oncorhynchus mykiss). Animal Nutrition. 7(4):1360-1370. https://doi.org/10.1016/j.aninu.2021.07.008.

Insect meal inclusion as a novel feed ingredient in soya-based diets improves performances of rainbow trout (Oncorhynchus mykiss) - (Peer Reviewed Journal)
Hossain, S., Fawole, F., Labh, S., Small, B., Overturf, K.E., Kumar, V. 2021. Insect meal inclusion as a novel feed ingredient in soya-based diets improves performances of rainbow trout (Oncorhynchus mykiss). Aquaculture. 544:737096. https://doi.org/10.1016/j.aquaculture.2021.737096.

Effects of elevated temperature and dietary additives Thermal Care™, Bio-Mos®, and GroBiotic® A on rainbow trout (Oncorhynchus mykiss) performance - (Peer Reviewed Journal)
Brokus, A., Rawles, S.D., Sealey, W.M., Conley, Z.B., Gaylord, T.G. 2021. Effects of elevated temperature and dietary additives Thermal Care™, Bio-Mos®, and GroBiotic® A on rainbow trout (Oncorhynchus mykiss) performance. Aquaculture. 544:Article e737084. Available: https://doi.org/10.1016/j.aquaculture.2021.737084.

Soybean trypsin inhibitor and urease activities and their correlations as affected by heating method, duration, sample matrix and prior soaking - (Peer Reviewed Journal)
Liu, K., Ruiz, N. 2021. Soybean trypsin inhibitor and urease activities and their correlations as affected by heating method, duration, sample matrix and prior soaking. Journal of the American Oil Chemists' Society. https://doi.org/10.1002/aocs.12514.

High moisture extrusion - a promising new method for making aquafeed with unique properties - (Trade Journal)
Liu, K. 2021. High moisture extrusion - a promising new method for making aquafeed with unique properties. International Aquafeed Magazine. Available: https://issuu.com/aquafeed.com/docs/aquafeed_vol_13_issue_2__2021/21.

Dietary amino acid supplementation affects temporal expression of amino acid transporters and metabolic genes in selected and commercial strains of rainbow trout (oncorhynchus mykiss) - (Peer Reviewed Journal)
Brezas, A., Kumar, V., Overturf, K.E., Hardy, R. 2021. Dietary amino acid supplementation affects temporal expression of amino acid transporters and metabolic genes in selected and commercial strains of rainbow trout (oncorhynchus mykiss). Aquaculture. 255. Article 110589. https://doi.org/10.1016/j.cbpb.2021.110589.

Trypsin inhibitor assay: expressing, calculating, and standardizing inhibitor activity in absolute amounts of trypsin inhibited or trypsin inhibitors - (Peer Reviewed Journal)
Liu, K. 2021. Trypsin inhibitor assay: expressing, calculating and standardizing inhibitor activity in absolute amounts of trypsin or trypsin inhibitors. Journal of the American Oil Chemists' Society. 98:355-373. https://doi.org/10.1002/aocs.12475.

Comparison of new and conventional processing methods for their effects on physical properties of fish feed - (Peer Reviewed Journal)
Liu, K., Frost, J.B., Welker, T.L., Barrows, F. 2021. Comparison of new and conventional processing methods for their effects on physical properties of fish feed. Animal Feed Science and Technology. 273. Article 114818. https://doi.org/10.1016/j.anifeedsci.2021.114818.

An international collaborative study on trypsin inhibitor assay for legumes, cereals and related products - (Peer Reviewed Journal)
Liu, K., Seegers, S., Cao, W., Wanasundara, J., Chen, J., Da Silva, A.E., Ross, C., Franco, A.L., Vrijenhoek, T., Bhowmik, P., Li, Y., Wu, X., Bloomer, S. 2021. An international collaborative study on trypsin inhibitor assay for legumes, cereals and related products. Journal of the American Oil Chemists' Society. 98:375-390. https://doi.org/10.1002/aocs.12459|.

Insect (black soldier fly, Hermetia illucens) meal supplementation prevents the soybean meal-induced intestinal enteritis in rainbow trout and health benefits of using insect oil - (Peer Reviewed Journal)
Kumar, V., Fawole, F., Romano, N., Hossain, S., Labh, S., Overturf, K.E., Small, B. 2020. Black soldier fly (Hermetia illucens) larvae (BSFL) supplementations prevented soybean meal-induced intestinal enteritis in rainbow trout and benefits of using BSFL oil. Fish and Shellfish Immunology. 109:116-124. https://doi.org/10.1016/j.fsi.2020.12.008.

Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition - (Peer Reviewed Journal)
Liu, K., Wise, M.L. 2021. Distributions of nutrients and avenanthramides within oat grain and effects on pearled kernel composition. Food Chemistry. 336. https://doi.org/10.1016/j.foodchem.2020.127668.

Evaluation of graded levels of soy oil as a fish oil replacement in high soy protein feeds for juvenile red drum, Sciaenops ocellatus - (Peer Reviewed Journal)
Watson, A., Napolitano, M., Schock, T., Bowden, J., Frost, J.B., Yost, J., Denson, M. 2020. Evaluation of graded levels of soy oil as a fish oil replacement in high soy protein feeds for juvenile red drum, Sciaenops ocellatus. Aquaculture. 529. Article 735627. https://doi.org/10.1016/j.aquaculture.2020.735627.