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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Research Project #434315

Research Project: Cranberry Genetics and Insect Management

Location: Vegetable Crops Research

Project Number: 5090-21220-004-00-D
Project Type: In-House Appropriated

Start Date: Mar 7, 2018
End Date: Mar 6, 2023

Objective:
Objective 1: Map and identify genes that underlie cranberry yield and quality traits, and explain the phenotypic differences between selected genotypes using genetic, genomic, and molecular approaches. Objective 2: Develop new enhanced cranberry germplasm and cultivars by integrating genetic and genomic breeding approaches with conventional cranberry breeding. Objective 3: Develop tools for the early detection and prevention of new, emerging cranberry pests (insects and mites). Sub-objective 3. Develop bio-insecticides using newly discovered, native nematode species. Objective 4: Develop new integrated pest management technologies for pest management and sustainable production of cranberry. Sub-objective 4.A. Develop a multi-species mating disruption program for the major moth pests of U.S. cranberries. Sub-objective 4.B. Investigate the biology and ecology of native pollinators to ensure the sustainable production of cranberries. Objective 5: Develop alternative cranberry production practices that improve water conservation and decrease plant disease. [NP301 C1 PS1B C2 PS2A] Expected benefits include a systems approach to cranberry production that includes genetic improvement, genomics, disease and pest mitigation, and water conservation.

Approach:
Objective 1: A multi-pedigree QTL mapping approach will be used to map cranberry yield and fruit quality traits. Phenotypic trait data collection will include traditional and newly developed high-throughput methodologies to measure yield and fruit quality related traits and other horticultural measurements, including total fruit weight, percent rotten fruit, average berry weight, and other fruit quality parameters such as TAcy and firmness. A composite SSR/SNP high-resolution cranberry genetic map developed based on three half-sibling populations will be used for QTL analysis. Objective 2: This research will collaborate with cranberry growers to establish a cranberry research station in Wisconsin and to establish various sized research plots to test the horticultural needs and performance of a selection of important cranberry cultivars. Phenotypic information that will be collected will be determined based on previous research to include the best traits to measure yield and quality. Additionally, a classic inbred-hybrid system will be used based on the best performing cranberry cultivars in the industry to develop improved cranberry lines and varieties in terms of yield and quality. Prior to creating cranberry inbreds and hybrids, horticultural, genetic, and genomic information will be carefully considered to ensure that strategic crosses are accomplished. Objective 3: A novel, effective bio-insecticide will be developed for arthropod pest suppression in commercial cranberry marshes. Two highly virulent nematode species, both native to Wisconsin, will comprise the bio-insecticide, and the nematode blend will ultimately be developed such that it can be applied at large-scales using standard spray equipment. Arthropod population suppression will be assessed among pest species and non-target species alike. Objective 4: A multi-species mating disruption system will be developed to control the top three insect pests of Wisconsin cranberries. The sex pheromones of these insect species will be loaded into carriers that can be applied efficiently via standard fertilizer-application equipment. We will also examine the capacity of the cranberry plant to prime its chemical defenses after 'eavesdropping' on the pheromones of its major pests. Bee-microbe symbioses will be investigated as a means to better understand and protect the native pollinators of cranberries.