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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Foodborne Toxin Detection and Prevention Research » People & Locations » Xiaohua He
Xiaohua He
Foodborne Toxin Detection and Prevention Research
Research Molecular Biologist

Phone: (510) 559-5823
Fax: (510) 559-6429

USDA, ARS, PWA, WRRC-FTDP
800 BUCHANAN ST
ALBANY , CA 94710
Projects
Advance the Development of Technologies for Detecting and Determining the Stability and Bioavailability of Toxins that Impact Food Safety and Food Defense
In-House Appropriated (D)
  Accession Number: 430056

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype -
Bai, X., Fu, S., Zhang, J., Fan, R., Xu, Y., Sun, H., He, X., Xu, J., Xiong, Y. 2018. Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype. Scientific Reports. 8:6756. https://doi.org/10.1038/s41598-018-25233-x.
An improved method for detection of Shiga toxin 2 in human serum -
He, X., Ardissino, G., Patfield, S.A., Cheng, L.W., Silva, C.J., Brigotti, M. 2018. An improved method for detection of Shiga toxin 2 in human serum. Toxins. 10(2):59. https://doi.org/10.3390/toxins10020059.
Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products -
Hu, L., Ma, L., Zheng, S., He, X., Hammack, T.S., Brown, E.W., Zhang, G. 2018. Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products. Food Control. 88:190-197. https://doi.org/10.1016/j.foodcont.2018.01.006.
Detection of abrin holotoxin using novel monoclonal antibodies -
He, X., Patfield, S.A., Cheng, L.W., Stanker, L.H., Rasooly, R., McKeon, T.A., Zhang, Y., Brandon, D.L. 2017. Detection of abrin holotoxin using novel monoclonal antibodies. Toxins. 9(12):386. https://doi.org/10.3390/toxins9120386.
TCR-Vß8 as alternative to animal testing for quantifying active SEE -
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2017. TCR-Vß8 as alternative to animal testing for quantifying active SEE. Journal of Environmental and Analytical Toxicology. 7(6):527. https://doi.org/10.4172/2161-0525.1000527.
Screening for the presence of mcr-1/mcr-2 genes in Shiga toxin-producing Escherichia coli recovered from a major produce-production region in California -
Mavrici, D., Yambao, J.C., Lee, B.G., Quinones, B., He, X. 2017. Screening for the presence of mcr-1/mcr-2 genes in Shiga toxin-producing Escherichia coli recovered from a major produce-production region in California. PLoS One. 12(11):e0187827. https://doi.org/10.1371/journal.pone.0187827.
A monoclonal-monoclonal antibody based capture elisa for abrin -
Tam, C.C., Cheng, L.W., He, X., Merrill, P.A., Hodge, D., Stanker, L.H. 2017. A monoclonal-monoclonal antibody based capture elisa for abrin. Toxins. 9(10):328. https://doi.org/10.3390/toxins9100328.
Abrin toxicity and bioavailability after temperature and pH treatment -
Tam, C.C., Henderson, T.D., Stanker, L.H., He, X., Cheng, L.W. 2017. Abrin toxicity and bioavailability after temperature and pH treatment. Toxins. 9(10):320. https://doi.org/10.3390/toxins9100320.
Complete Genome Sequence of a Shiga toxin-producing Enterobacter cloacae Clinical Isolate -
Carter, M.Q., Pham, A.C., Huynh, S., He, X. 2017. Complete Genome Sequence of a Shiga toxin-producing Enterobacter cloacae Clinical Isolate. Genome Announcements. 5(37):e00883-17. https://doi.org/10.1128/genomeA.00883-17.
Novel monoclonal antibodies against Stx1d and 1e and their use for improvement of immunoassays Reprint Icon -
He, X., Patfield, S.A., Rasooly, R., Mavrici, D. 2017. Novel monoclonal antibodies against Stx1d and 1e and their use for improvement of immunoassays. Journal of Immunological Methods. 44:52-56.
Overcoming the challenges of safely quantifying and distinguishing among Shiga toxins in complex media and human serum -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X., Wu, V.C. 2017. Overcoming the challenges of safely quantifying and distinguishing among Shiga toxins in complex media and human serum. Meeting Abstract. Poster. ARS-FSIS 2017 meeting.
Evaluation of ELISA tests specific for Shiga toxin 1 and 2 in food and water samples Reprint Icon -
Gehring, A.G., Fratamico, P.M., Lee, J., Ruth, L., He, X., He, Y., Paoli, G., Stanker, L.H., Rubio, F.M. 2017. Evaluation of ELISA tests specific for Shiga toxin 1 and 2 in food and water samples. Food Control. 77:145-149.
Host cell interactions of outer membrane vesicle-associated virulence factors of Enterohemorrhagic Escherichia coli O157: intracellular delivery, trafficking and mechanisms of cell injury -
Bielaszewska, M., Ruter, C., Bauwens, A., Greune, L., Zhang, W., He, X., Lloubes, R., Fruth, A., Kim, K.S., Schmidt, M., Dobrindt, U., Mellmann, A., Karch, H. 2017. Host cell interactions of outer membrane vesicle-associated virulence factors of Enterohemorrhagic Escherichia coli O157: intracellular delivery, trafficking and mechanisms of cell injury. PLoS Pathogens. 13(2):e1006159. doi: 10.1371/journal. ppat. 1006159.
Detecting and distinguishing among type 1 and type 2 Shiga toxins in human serum -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2017. Detecting and distinguishing among type 1 and type 2 Shiga toxins in human serum. Abstracts of the Papers of the American Chemical Society. 253:234-AGFD.
Evaluation of 3M molecular detection system and ANSR pathogen detection system for rapid detection of salmonella from egg products Reprint Icon -
Hu, L., Ma, L., Zheng, S., He, X., Wang, H., Brown, E., Hammack, T., Zhang, G. 2016. Evaluation of 3M molecular detection system and ANSR pathogen detection system for rapid detection of salmonella from egg products. Poultry Science. 96(5):1410-1418. doi: 10.3382/ps/pew399.
Shiga toxins: A review of structure, mechanism, and detection -
Silva, C.J., Brandon, D.L., Skinner, C.B., He, X. 2017. Shiga toxins: A review of structure, mechanism, and detection. Cham, Switzerland: Springer International Publishing. 118 p.
Volatile compounds and odor traits of dry-cured ham (Prosciutto crudo) irradiated by electron beam and gamma ray Reprint Icon -
Kong, Q., Yan, W., Yue, L., Chen, Z., Wamg, H., Qi, W., He, X. 2016. Volatile compounds and odor traits of dry-cured ham (Prosciutto crudo) irradiated by electron beam and gamma ray. Journal of Radiation Physics and Chemistry. 130:265-272. doi: 10.1016/j.radphyschem.2016.09.0080969-806X.
Validation of two new immunoassays for sensative detection of a broad range of shiga toxins Reprint Icon -
Kong, Q., Patfield, S.A., Skinner, C.B., Stanker, L.H., Gehring, A.G., Fratamico, P.M., Rubio, F., Qi, W., He, X. 2016. Validation of two new immunoassays for sensative detection of a broad range of shiga toxins. Austin Immunology. 1(2):1007.
Detecting and discriminating among Shiga toxins -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X., Wu, V.C. 2016. Detecting and discriminating among Shiga toxins. doi: 10.1021/acs.jafc.6b00379.
Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains Reprint Icon -
Khalil, R., Skinner, C.B., Patfield, S.A., He, X. 2016. Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains. Pathogens and Disease. 74(5):1-11. doi: 10.1093/femspd/ftw037.
Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum -
Wu, V.C., Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2016. Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum. ARS Food Safety and Inspection Service Research Workshop. ARS-FSIS.
New monoclonal antibodies against a novel subtype of Shiga toxin 1 produced by Enterobacter cloacae and their use in analysis of human serum Reprint Icon -
Skinner, C.B., Patfield, S.A., Khalil, R., Kong, Q., He, X. 2016. New monoclonal antibodies against a novel subtype of Shiga toxin 1 produced by Enterobacter cloacae and their use in analysis of human serum. mSphere. 1(1):e00099-15.
A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef Reprint Icon -
He, X., Kong, Q., Patfield, S.A., Skinner, C.B., Rasooly, R. 2016. A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef. PLoS One. 11(1):e0148092.
Improved method for extraction of castor seed for toxin determination -
McKeon, T.A., Brandon, D.L., He, X. 2015. Improved method for extraction of castor seed for toxin determination. Biocatalysis and Agricultural Biotechnology. 5:56-57. doi: 10.1016/j.bcab.2015.12.007.
Analysis of castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA) -
Brandon, D.L., McKeon, T.A., Patfield, S.A., He, X. 2016. Analysis of castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA). Journal of the American Oil Chemists' Society. 93:359-363.
An environmental shiga toxin-producing Escherichia coli O145 clonal population exhibits high-level phenotypic variation that includes virulence traits -
Carter, M.Q., Quinones, B., He, X., Louie, J.W., Zhong, W.W., Lee, B.G., Yambao, J.C., Mandrell, R.E., Cooley, M.B. 2015. An environmental shiga toxin-producing Escherichia coli O145 clonal population exhibits high-level phenotypic variation that includes virulence traits. Applied and Environmental Microbiology. doi: 10.1128/AEM.03172-15.
Evaluation of castor oil samples for potential toxin contamination -
Mckeon, T.A., Patfield, S.A., He, X. 2016. Evaluation of castor oil samples for potential toxin contamination. Industrial Crops and Products. 93:299-301.
Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2015. Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum. Toxins. 7:5236-5253.
Immuno-PCR assay for sensitive detection of proteins in real time -
He, X., Patfield, S.A. 2016. Immuno-PCR assay for sensitive detection of proteins in real time. Methods in Molecular Biology. 1318:139-148. doi: 10.1007/978-1-4939-2742-5_14.
Serum Shiga toxin 2 values in patients during the acute phase of post-diarrheal hemolytic uremic syndrome -
Serum Shiga toxin 2 values in patients during the acute phase of diarrhoea-associated hemolytic uremic syndrome Reprint Icon -
He, X., Quinones, B., Te Loo, M., Loos, S., Scavia, G., Brigotti, M., Levtchenko, E., Monnens, L. 2015. Serum Shiga toxin 2 values in patients during the acute phase of diarrhoea-associated hemolytic uremic syndrome. Acta Paediatrica. 104(12):e564-568.
Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104:H4 outbreak strain Reprint Icon -
Kunsmann, L., Ruter, C., Bauwens, A., Greune, L., Gluder, M., Kemper, B., Fruth, A., Wai, S., He, X., Lloubes, R., Schmidt, A.M., Dobrindt, U., Mellmann, A., Karch, H., Bielaszewska, M. 2015. Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104:H4 outbreak strain. Scientific Reports. doi: 10.1038/srep13252.
An in vitro combined antibiotic/antibody treatment eliminates toxicity from Shiga toxin-producing E. coli Reprint Icon -
Skinner, C.B., Patfield, S.A., He, X., Zhang, G. 2015. An in vitro combined antibiotic/antibody treatment eliminates toxicity from Shiga toxin-producing E. coli. Antimicrobial Agents and Chemotherapy. 59(9):5435-5444.
New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes Reprint Icon -
Skinner, C.B., Patfield, S.A., Hernlem, B.J., He, X. 2015. New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes. PLoS One. 10(7):e0132419.
Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices -
Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Hnasko, R.M. 2015. Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices. Toxins. Toxins 7:1163-1173.
Detection of shiga toxins by lateral flow assay -
Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Mcgarvey, J.A., Hnasko, R.M. 2015. Detection of shiga toxins by lateral flow assay. Toxins. 7:1163-1173. doi: 10.3390/Toxins7041163.
Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin Reprint Icon -
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2015. Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin. Toxins. 7(3):835-845. doi: 10.3390/toxins7030835.
Castor diacylglycerol acyltransferase type1(DGAT1)displays greater activity with diricinolein than Arabidopsis DGAT1 -
Mckeon, T.A., He, X. 2015. Castor diacylglycerol acyltransferase type1(DGAT1)displays greater activity with diricinolein than Arabidopsis DGAT1. Biocatalysis and Agricultural Biotechnology. 4:276-278.
Toxin content of commercial castor cultivars Reprint Icon -
Mckeon, T.A., Auld, D., Brandon, D.L., Leviatov, S., He, X. 2014. Toxin content of commercial castor cultivars. Journal of the American Oil Chemists' Society. DOI: 10.1007/s11746-014-2505-3.
A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins Reprint Icon -
Gehring, A.G., He, X., Fratamico, P.M., Lee, J., Bagi, L.K., Brewster, J.D., Paoli, G., He, Y., Xie, Y., Skinner, C.B., Barnett, C., Harris, D. 2014. A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins. Toxins. 6:1855-1872.
New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo -
Skinner, C.B., Patfield, S.A., Stanker, L.H., Fratamico, P.M., He, X. 2014. New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo. PLoS One. 9(6):e99854. DOI:10.1371journal.pone0099854.
Current technologies for detection of ricin in different matrices -
He, X. 2014. Current technologies for detection of ricin in different matrices.Cherwonogrodky, J.W., editor. Ricin Toxin. Oak Park, Illinois:Bentham Science. p. 38-56.
Risk of ricin from commercial castor production in North America -
Auld, D.L., Trostle, C.L., Miller, T.D., He, X., Duncan, R.W. 2014. Risk of ricin from commercial castor production in North America. Cherwonogrodky, J.W., editor. Ricin Toxin. Oak Park, Illinois:Bentham Science. p. 86-97.
Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I., Hui, C., Patfield, S.A., Carter, J.M., He, X. 2014. Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts. Analytical Chemistry. 86(10):4698-4706.
Electron-beam is effective in reducing Escherichia coli on blueberries and extending the shelf life of the fruits Reprint Icon -
Kong, Q., Wu, A., Qi, W., Qi, R., Carter, J.M., Rasooly, R., He, X. 2014. Electron-beam is effective in reducing Escherichia coli on blueberries and extending the shelf life of the fruits. Postharvest Biology and Technology. 95(2014):28-35.
Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range. Reprint Icon -
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I.A., Hui, C., Patfield, S.A., He, X. 2014. Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range [abstract]. Meeting Abstract. Innovations in Mass Spectrometry. Paper No. 355.
Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium Reprint Icon -
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2014. Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium. Journal of Agricultural and Food Chemistry. 62:3301-3305 DOI.org/10.1021/jf500278a.
Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies Reprint Icon -
Cheng, L.W., Henderson II, T.D., Patfield, S.A., Stanker, L.H., He, X. 2013. Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies. Infection and Immunity. 5:1845-1858. DOI: 10.3390/toxins5101845.
Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f -
Skinner, C.B., Patfield, S.A., Stanker, L.H., He, X. 2013. Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f. PLoS One. 8(9):e76563 DOI: 10.1371/journal/pone.0076563.
A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples -
He, X., Patfield, S.A., Hnasko, R.M., Rasooly, R., Mandrell, R.E. 2013. A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples. PLoS One. 8(10):e76368.
Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells Reprint Icon -
Rasooly, R., Hernlem, B.J., He, X. 2013. Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells. Toxins. 5:1447-1461. doi:10.3390/toxins5081447.
Detection of bacterial toxins by lateral flow immunoassay -
Ching, K.H., Stanker, L.H., He, X., Hnasko, R.M. 2013. Detection of bacterial toxins by lateral flow immunoassay. p. 1-28. Irvine, CA; Biodot.
Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2 Reprint Icon -
Skinner, C.B., Patfield, S.A., Rasooly, R., Carter, J.M., He, X. 2013. Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2. PLoS One. 8(3):e59760.
Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk -
He, X., Mcmahon, S.A., Skinner, C.B., Merrill, P.A., Scotcher, M., Stanker, L.H. 2013. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. Journal of Immunological Methods. 389(1-2):18-28. doi:10.1016/j.jim.2012.12.005.
Reducing the toxicity of castor seed meal through processing treatments -
McKeon, T.A., Shim, K., He, X. 2012. Reducing the toxicity of castor seed meal through processing treatments. Biocatalysis and Agricultural Biotechnology. doi.org/10.1016/j.bcab.2012.12.001.
A review on the challenges for increassed production of castor -
Severino, L.S., Auld, D.L., Baldanzi, M., Chen, G.Q., He, X., Crosby, W., Machado, O.L., Morris, J.B., Zieler, H. 2012. A review on the challenges for increassed production of castor. Agronomy Journal. 104(4):853-880.
Replacement of animals in assays for detection of toxins in food(Abstract) -
Detoxification of castor meal through reactive seed crushing -
Dubois, J., Piccirilli, A., Magne, J., He, X. 2012. Detoxification of castor meal through reactive seed crushing. Industrial Crops and Products. 43(1):194-199.
Milk inhibits the biological activity of ricin -
Rasooly, R., He, X., Friedman, M. 2012. Milk inhibits the biological activity of ricin. Journal of Biological Chemistry. 287(33):27924-27929. doi:10.1074/jbc.M112.362988.
A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli -
He, X., Quinones, B., Mcmahon, S.A., Mandrell, R.E. 2012. A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli. Toxins. 4(7):487-504. doi:10.3390/toxins4070487
Sensitive bioassay for detection of biologically active ricin in food -
Rasooly, R., He, X. 2012. Sensitive bioassay for detection of biologically active ricin in food. Journal of Food Protection. 75(5):951-954.
Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum -
He, X., Mcmahon, S.A., Rasooly, R. 2012. Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum. Biocatalysis and Agricultural Biotechnology. 1(2):105-109. doi:10.1016/j.bcab.2011.08.016.
Sensitive detection of Shiga toxin 2 and some of its Stx 2 variants in environmental samples by a novel immuno-PCR assay -
He, X., Qi, W., Quinones, B., Mcmahon, S.A., Cooley, M.B., Mandrell, R.E. 2011. Sensitive detection of Shiga toxin 2 and some of its Stx 2 variants in environmental samples by a novel immuno-PCR assay. Applied and Environmental Microbiology. 77(1):3558-3564. doi:10.1128/AEM.02205-10.
Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR -
He, X., Mcmahon, S.A., Henderson II, T.D., Griffey, S.M., Cheng, L.W. 2010. Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR. PLoS One. 5(9):e12858. doi:10.1371/journal.pone.0012858.
Development of a Novel Immuno-PCR Assay for Detection of Ricin in Ground Beef, Liquid Chicken Egg and Milk -
He, X., McMahon, S.A., McKeon, T.A., Brandon, D.L. 2010. Development of a Novel Immuno-PCR Assay for Detection of Ricin in Ground Beef, Liquid Chicken Egg and Milk. Journal of Food Protection. 73(4):695-700
Simple method to detect triacylglycerol biosynthesis in a yeast-based recombinant system -
Siloto, R.M., Truksa, M., He, X., McKeon, T.A., Weselake, R. 2009. Simple method to detect triacylglycerol biosynthesis in a yeast-based recombinant system. Lipids. 44:963-973.
Detection of Protein Toxins -
Brandon, D.L., Cheng, L.W., He, X., Rasooly, R., Scotcher, M.C., Stanker, L.H., Carter, J.M. 2009. Detection of Protein Toxins. Meeting Abstract. Poster No. 8.
Validation of a Cell-Free Translation Assay for Detecting Shiga Toxin 2 in Bacterial Culture -
He, X., Quinones, B., Carter, J.M., Mandrell, R.E. 2009. Validation of a Cell-Free Translation Assay for Detecting Shiga Toxin 2 in Bacterial Culture. Journal of Agriculture and Food Chemistry. 57(11):5084-5088
Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods -
Carter, J.M., Cheng, L.W., He, X., Rasooly, R., Brandon, D.L., Stanker, L.H. 2010. Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods. Proceedings from Biodetection Technologies 6th Edition. 5:75-95.
Global challenges/chemistry solutions: Promoting personal safety and national security -
Effect of food matrices on the biological activity of ricin -
He, X., Lu, S.S., Cheng, L.W., Rasooly, R., Carter, J.M. 2008. Effect of food matrices on the biological activity of ricin. Journal of Food Protection 71(10):2053-2058.
Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay -
Rasooly, R., Stanker, L.H., Carter, J.M., Do, P.M., Cheng, L.W., He, X., Brandon, D.L. 2008. In vitro peptide cleavage assay for detection of Botulinum Neurotoxin-A activity in food. International Journal of Food Microbiiology. 126 (2008):135-139
Ricinus communis contains and acyl-CoA synthetase that preferentially activates ricinoleate to its CoA thioester -
He, X., Chen, G.Q., Kang, S.T., Mckeon, T.A. 2007. Ricinus communis contains and acyl-CoA synthetase that preferentially activates ricinoleate to its CoA thioester. Lipids Journal. 42:931-938.
Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs -
He, X., Carter, J.M., Brandon, D.L., Cheng, L.W., Mckeon, T.A. 2007. Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs. Journal of Agricultural and Food Chemistry. 55:6897-6902
Molecular Approaches for the Identification of Toxin in Foods -
Synthesis of Novel Acylglycerol Substrates for Acyltransferases. -
McKeon, T.A., Kang, S., He, X., Lin, J.T., Turner, C. 2007. Synthesis of Novel Acylglycerol Substrates for Acyltransferases.. Proceedings JOCS/AOCS World Congress.
Expression Profiles of Genes Involved in Fatty Acid and Triacyblycerol Synthesis in Castor Bean (Ricinus communis L.) -
Chen, G.Q., Turner, C., He, X., Nguyen, T.T., McKeon, T.A., Chingcuanco, D.L. 2007. Expression Profiles of Genes Involved in Fatty Acid and Triacyblycerol Syntheses in Castor Bean (Ricinus communis L.). Lipids. 41:263-274.
Detection of castor contamination by real-time polymerase chain reaction -
He, X., Brandon, D.L., Chen, G.Q., McKeon, T.A., Carter, J.M. 2007. Detection of castor contamination by real-time polymerase chain reaction. Journal of Agricultural and Food Chemistry. 55(2):545-550.
Castor oil: Biosynthesis and Uses -
Mckeon, T.A., He, X., Chen, G.Q., Lin, J.T. 2007. Castor oil: Biosynthesis and Uses. 226th American Chemical Society National Meeting.
The Enzymology of Castor Oil Biosynthesis -
McKeon, T.A., He, X., Chen, G.Q., Lin, J.T. 2006. The Enzymology of Castor Oil Biosynthesis. Association for the Advancement of Industrial Crops Conference.
BIOAVAILABILITY OF CRUDE RICIN IN FOOD MATRICES -
Cheng, L.W., Brandon, D.L., He, X., Chen, G.Q., Carter, J.M. 2006. Bioavailability of crude ricin in food matrices. [Abstract]. ACS National Meeting and Exposition. Poster AGFD 64
DEVELOPMENT OF MONOCLONAL ANTIBODIES SPECIFIC FOR RICIN -
Brandon, D.L., Cheng, L.W., He, X., Carter, J.M. 2006. Development of monoclonal antibodies specific for ricin. [Abstract]. ACS National Meeting and Exposition. Oral Presentation AGRO 234
DETECTION OF CASTOR CONTAMINATION IN GROUND BEEF BY REAL-TIME PCR -
He, X., Brandon, D.L., Chen, G.Q., Mckeon, T.A., Carter, J.M. 2006. Detection of castor contamination in ground beef by real-time pcr. [Abstract]. ACS National Meeting and Exposition. Poster AGFD 18.
DIACYLGLYCEROL ACYLTRANSFERASE ACTIVITY AND TRIACYLGLYCEROL SYNTHESIS IN GERMINATING CASTOR SEED COTYLEDONS -
He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2006. Diacylglycerol acyltransferase activity and triacylglycerol synthesis in germinating castor seed cotyledons. Lipids. Vol. 41, Number 3: 281-285, 2006.
TOWARD THE DEVELOPMENT OF A SAFE CASTOR CROP: EXPRESSION PATTERN COMPARISON OF RICIN AND 2S ALBUMIN GENES AND PHYLOGENY ANALYSIS OF 2S ALBUMINS -
Chen, G.Q., He, X., Ahn, Y., Vang, L., Mckeon, T.A. 2006. Toward the development of a safe castor crop: expression pattern comparison of ricin and 2s albumin genes and phylogeny analysis of 2s albumins. Recent Progress in Medicinal Plants. 15: pp. 453-463
SELECTIVE HYDROLYSIS OF POLAR TRIACYLGLYCEROLS BY LIPASES -
McKeon, T.A., Kang, S., Turner, C., He, X., Lin, J.W. 2005. Selective Hydrolysis of Polar Triacylglycerols by Lipases. Pacifichem Symposium.
CASTOR OIL BOISYNTHESIS AND EXPRESSION PROFILES OF LIPID GENES IN DEVELOPING SEED OF RICINUS COMMUNIS L. (CASTOR BEAN) -
Chen, G.Q., Turner, C., He, X., Mckeon, T.A. 2005. Castor oil boisynthesis and expression profiles of lipid genes in developing seed of ricinus communis l. (castor bean). Meeting Abstract.
QUANTITATIVE TRANSCRIPT PROFILING OF LIPID BIOSYNTHESIS GENES IN DEVELOPING CASTOR SEEDS. -
Chen, G.Q., Ahn, Y., He, X., Mckeon, T.A. 2005. Quantitative transcript profiling of lipid biosynthesis genes in developing castor seeds.. National Plant Lipid Cooperative Meeting.
ENDOSPERM MORPHOLOGY AND TRANSCRIPT PROFILES OF STORAGE PROTEINS IN DEVELOPING CASTOR SEEDS. -
Chen, G.Q., Ahn, Y., He, X., Mckeon, T.A. 2005. Endosperm morphology and transcript profiles of storage proteins in developing castor seeds.. World Congress on In Vitro Biology.
MOLECULAR CHARACTERIZATION OF THE ACYL-COA-DEPENDENT DIACYLGLYCEROL ACYLTRANSFERASE IN PLANTS -
He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2005. Molecular characterization of the acyl-coa-dependent diacylglycerol acyltransferase in plants. Research Signpost: Recent Research Developments in Applied Microbiology and Biotechnology. 2:69-86.
EFFECT OF DIACYLGLYCEROL ACYLTRANSFERASE ON LIPID METABOLISM IN DEVELOPING SEEDS OF CASTOR -
He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2005. Effect of diacylglycerol acyltransferase on lipid metabolism in developing seeds of castor. Annual Meeting and Expo of the American Oil Chemists' Society.
A SIMPLE AND SENSITIVE ASSAY FOR DISTINGUISHING THE EXPRESSION OF RICIN AND RICINUS COMMUNIS AGGLUTININ GENE IN DEVELOPING CASTOR SEED (RICINUS COMMUNIS L.) -
Chen, G.Q., He, X., McKeon, T.A. 2005. A simple and sensitive assay for distinguishing the expression of ricin and ricinus communis agglutinin gene in developing castor seed (Ricinus communis L.). Journal of Agricultural and Food Chemistry. 53(6):2358-2361.
REGULATION OF DIACYLGLYCEROL ACYLTRANSFERASE IN DEVELOPING SEEDS OF CASTOR -
He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2005. Regulation of diacylglycerol acyltransferase in developing seeds of castor. Lipids. 39: pp. 865-871
2S ALBUMIN GENE EXPRESSION IN CASTOR PLANT (RICINUS COMMUNIS L.) -
Chen, G.Q., He, X., Liao, L., McKeon, T.A. 2004. 2s Albumin Gene Expression in Castor Plant (Ricinus communis L.). Journal of the American Oil Chemists' Society. 81(9):867-872.
CLONING AND CHARACTERIZATION OF CASTOR DGAT -
He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2004. Cloning and characterization of castor dgat. Lipids Journal.39:311-318
APPLICATION OF LIPASES TO DEVELOP NOVEL ACYLTRANSFERASE SUBSTRATES -
Mckeon, T.A., Turner, C., He, X., Chen, G.Q., Lin, J.T. 2003. Application of lipases to develop novel acyltransferase substrates. Meeting Abstract.
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