Xiaohua He
Foodborne Toxin Detection and Prevention Research
Research Molecular Biologist
Phone: (510) 559-5823
Fax: (510) 559-6429
USDA, ARS, PWA, WRRC-FTDP
800 BUCHANAN ST
ALBANY, CA 94710
Publications
(Clicking on the reprint icon
will take you to the publication reprint.)
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DNA Adenine Methylase, not the PstI restriction-modification system, regulates virulence gene expression in Shiga toxin-producing Escherichia coli
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(Peer Reviewed Journal)
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Carter, M.Q., Pham, A., Huynh, S., Parker, C., Miller, A., He, X., Hu, B., Chan, P.S. 2020. DNA Adenine Methylase, not the PstI restriction-modification system, regulates virulence gene expression in Shiga toxin-producing Escherichia coli. Food Microbiology. 96(2021)103722. https://doi.org/10.1016/j.fm.2020.103722.
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Differential induction of Shiga toxin in environmental Escherichia coli O145:H28 strains carrying the same genotype as the outbreak strains
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(Peer Reviewed Journal)
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Carter, M.Q., Pham, A.C., Du, W.N., He, X. 2020. Differential induction of Shiga toxin in environmental Escherichia coli O145:H28 strains carrying the same genotype as the outbreak strains. International Journal of Food Microbiology. https://doi.org/10.1016/j.ijfoodmicro.2020.109029.
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Low prevalence of mobile colistin-resistance in U.S. meat, catfish, poultry and genomic characterization of a mcr-1 positive Escherichia coli strain
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(Peer Reviewed Journal)
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Wang, Y., Hou, N., Johnston, J., Sarreal, C., Jarosh, J., Hughes, A.C., Gu, Y.Q., He, X. 2020. Low prevalence of mobile colistin-resistance in U.S. meat, catfish, poultry and genomic characterization of a mcr-1 positive Escherichia coli strain. Food Control. 118:10734. https://doi.org/10.1016/j.foodcont.2020.107434.
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Genomic insight into natural inactivation of Shiga toxin 2 production in an environmental Escherichia coli strain producing Shiga toxin 1
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(Peer Reviewed Journal)
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Carter, M.Q., Pham, A.C., He, X., Hnasko, R.M. 2020. Genomic insight into natural inactivation of Shiga toxin 2 production in an environmental Escherichia coli strain producing Shiga toxin 1. Foodborne Pathogens and Disease. 17(9):555-567. https://doi.org/10.1089/fpd.2019.2767.
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Structural and functional characterization of Stx2k, a new subtype of Shiga toxin 2
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(Peer Reviewed Journal)
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Hughes, A.C., Zhang, Y., Bai, X., Xiong, Y., Wang, Y., Yang, X., Xu, Q., He, X. 2019. Structural and functional characterization of Stx2k, a new subtype of Shiga toxin 2. Microorganisms. 8(1):4. https://doi.org/10.3390/microorganisms8010004.
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Escherichia coli strains producing a novel Shiga toxin 2 subtype circulate in China
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(Peer Reviewed Journal)
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Yang, X., Bai, X., Zhang, J., Sun, H., Fu, S., Fan, R., He, X., Scheutz, F., Matussek, A., Xiong, Y. 2020. Escherichia coli strains producing a novel Shiga toxin 2 subtype circulate in China. International Journal of Medical Microbiology. 310(1):151377. https://doi.org/10.1016/j.ijmm.2019.151377.
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Particulate Shiga Toxin 2 in blood is associated to the development of hemolytic uremic syndrome in children
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(Peer Reviewed Journal)
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Brigotti, M., He, X., Carnicelli, D., Arfilli, V., Porcellini, E., Galassi, E., Tazzari, P., Ricci, F., Patfield, S.A., Testa, S., Paglialonga, F., Picicco, D., Caprioli, A., Scavia, G., Morabito, S., Ardissino, G. 2019. Particulate Shiga Toxin 2 in blood is associated to the development of hemolytic uremic syndrome in children. Thrombosis and Haemostasis. 120(1):107-120. https://doi.org/10.1055/s-0039-3400301.
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T cell receptor Vß9 in method to rapidly detect active staphylococcal enterotoxin type A without live animals
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(Peer Reviewed Journal)
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Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2019. T cell receptor Vß9 in method to rapidly detect active staphylococcal enterotoxin type A without live animals. Toxins. 11:399. https://doi.org/10.3390/toxins11070399.
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Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A
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(Peer Reviewed Journal)
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Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2018. Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A. Toxins. 10(12):540. https://doi.org/10.3390/toxins10120540.
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Soluble toll-like receptor 4 impairs the interaction of Shiga toxin 2a with human serum amyloid P component
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(Peer Reviewed Journal)
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Brigotti, M., Arfilli, V., Carnicelli, D., Ricci, F., Tazzari, P., Ardissino, G., Scavia, G., Morabito, S., He, X. 2018. Soluble toll-like receptor 4 impairs the interaction of Shiga toxin 2a with human serum amyloid P component. Toxins. 10(9):379. https://doi.org/10.3390/toxins10090379.
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Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
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(Peer Reviewed Journal)
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Bai, X., Fu, S., Zhang, J., Fan, R., Xu, Y., Sun, H., He, X., Xu, J., Xiong, Y. 2018. Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype. Scientific Reports. 8:6756. https://doi.org/10.1038/s41598-018-25233-x.
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An improved method for the sensitive detection of Shiga toxin 2 in human serum
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(Peer Reviewed Journal)
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He, X., Ardissino, G., Patfield, S.A., Cheng, L.W., Silva, C.J., Brigotti, M. 2018. An improved method for the sensitive detection of Shiga toxin 2 in human serum. Toxins. 10(2):59. https://doi.org/10.3390/toxins10020059.
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Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products
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(Peer Reviewed Journal)
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Hu, L., Ma, L., Zheng, S., He, X., Hammack, T.S., Brown, E.W., Zhang, G. 2018. Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products. Food Control. 88:190-197. https://doi.org/10.1016/j.foodcont.2018.01.006.
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Detection of abrin holotoxin using novel monoclonal antibodies
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(Peer Reviewed Journal)
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He, X., Patfield, S.A., Cheng, L.W., Stanker, L.H., Rasooly, R., McKeon, T.A., Zhang, Y., Brandon, D.L. 2017. Detection of abrin holotoxin using novel monoclonal antibodies. Toxins. 9(12):386. https://doi.org/10.3390/toxins9120386.
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TCR-Vß8 as alternative to animal testing for quantifying active SEE
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(Peer Reviewed Journal)
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Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2017. TCR-Vß8 as alternative to animal testing for quantifying active SEE. Journal of Environmental and Analytical Toxicology. 7(6):527. https://doi.org/10.4172/2161-0525.1000527.
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Screening for the presence of mcr-1/mcr-2 genes in Shiga toxin-producing Escherichia coli recovered from a major produce-production region in California
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(Peer Reviewed Journal)
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Mavrici, D., Yambao, J.C., Lee, B.G., Quinones, B., He, X. 2017. Screening for the presence of mcr-1/mcr-2 genes in Shiga toxin-producing Escherichia coli recovered from a major produce-production region in California. PLoS One. 12(11):e0187827. https://doi.org/10.1371/journal.pone.0187827.
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A monoclonal-monoclonal antibody based capture elisa for abrin
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(Peer Reviewed Journal)
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Tam, C.C., Cheng, L.W., He, X., Merrill, P.A., Hodge, D., Stanker, L.H. 2017. A monoclonal-monoclonal antibody based capture elisa for abrin. Toxins. 9(10):328. https://doi.org/10.3390/toxins9100328.
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Abrin toxicity and bioavailability after temperature and pH treatment
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(Peer Reviewed Journal)
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Tam, C.C., Henderson, T.D., Stanker, L.H., He, X., Cheng, L.W. 2017. Abrin toxicity and bioavailability after temperature and pH treatment. Toxins. 9(10):320. https://doi.org/10.3390/toxins9100320.
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Complete Genome Sequence of a Shiga toxin-producing Enterobacter cloacae Clinical Isolate
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(Peer Reviewed Journal)
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Carter, M.Q., Pham, A.C., Huynh, S., He, X. 2017. Complete Genome Sequence of a Shiga toxin-producing Enterobacter cloacae Clinical Isolate. Genome Announcements. 5(37):e00883-17. https://doi.org/10.1128/genomeA.00883-17.
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Novel monoclonal antibodies against Stx1d and 1e and their use for improvement of immunoassays
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(Peer Reviewed Journal)
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He, X., Patfield, S.A., Rasooly, R., Mavrici, D. 2017. Novel monoclonal antibodies against Stx1d and 1e and their use for improvement of immunoassays. Journal of Immunological Methods. 44:52-56.
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Overcoming the challenges of safely quantifying and distinguishing among Shiga toxins in complex media and human serum
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(Abstract Only)
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Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X., Wu, V.C. 2017. Overcoming the challenges of safely quantifying and distinguishing among Shiga toxins in complex media and human serum. Meeting Abstract. Poster. ARS-FSIS 2017 meeting.
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Evaluation of ELISA tests specific for Shiga toxin 1 and 2 in food and water samples
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(Peer Reviewed Journal)
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Gehring, A.G., Fratamico, P.M., Lee, J., Ruth, L., He, X., He, Y., Paoli, G., Stanker, L.H., Rubio, F.M. 2017. Evaluation of ELISA tests specific for Shiga toxin 1 and 2 in food and water samples. Food Control. 77:145-149.
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Host cell interactions of outer membrane vesicle-associated virulence factors of Enterohemorrhagic Escherichia coli O157: intracellular delivery, trafficking and mechanisms of cell injury
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(Peer Reviewed Journal)
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Bielaszewska, M., Ruter, C., Bauwens, A., Greune, L., Zhang, W., He, X., Lloubes, R., Fruth, A., Kim, K.S., Schmidt, M., Dobrindt, U., Mellmann, A., Karch, H. 2017. Host cell interactions of outer membrane vesicle-associated virulence factors of Enterohemorrhagic Escherichia coli O157: intracellular delivery, trafficking and mechanisms of cell injury. PLoS Pathogens. 13(2):e1006159. doi: 10.1371/journal. ppat. 1006159.
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Detecting and distinguishing among type 1 and type 2 Shiga toxins in human serum
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(Abstract Only)
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Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2017. Detecting and distinguishing among type 1 and type 2 Shiga toxins in human serum. Abstracts of the Papers of the American Chemical Society. 253:234-AGFD.
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Evaluation of 3M molecular detection system and ANSR pathogen detection system for rapid detection of salmonella from egg products
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(Peer Reviewed Journal)
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Hu, L., Ma, L., Zheng, S., He, X., Wang, H., Brown, E., Hammack, T., Zhang, G. 2016. Evaluation of 3M molecular detection system and ANSR pathogen detection system for rapid detection of salmonella from egg products. Poultry Science. 96(5):1410-1418. doi: 10.3382/ps/pew399.
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Shiga toxins: a review of structure, mechanism, and detection
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Silva, C.J., Brandon, D.L., Skinner, C.B., He, X. 2017. Shiga Toxins: a Review of Structure, Mechanism, and Detection. Cham, Switzerland: Springer International Publishing. 118 p.
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Volatile compounds and odor traits of dry-cured ham (Prosciutto crudo) irradiated by electron beam and gamma ray
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(Peer Reviewed Journal)
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Kong, Q., Yan, W., Yue, L., Chen, Z., Wamg, H., Qi, W., He, X. 2016. Volatile compounds and odor traits of dry-cured ham (Prosciutto crudo) irradiated by electron beam and gamma ray. Journal of Radiation Physics and Chemistry. 130:265-272. doi: 10.1016/j.radphyschem.2016.09.0080969-806X.
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Validation of two new immunoassays for sensative detection of a broad range of shiga toxins
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(Peer Reviewed Journal)
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Kong, Q., Patfield, S.A., Skinner, C.B., Stanker, L.H., Gehring, A.G., Fratamico, P.M., Rubio, F., Qi, W., He, X. 2016. Validation of two new immunoassays for sensative detection of a broad range of shiga toxins. Austin Immunology. 1(2):1007.
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Detecting and discriminating among Shiga toxins
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(Abstract Only)
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Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X., Wu, V.C. 2016. Detecting and discriminating among Shiga toxins. doi: 10.1021/acs.jafc.6b00379.
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Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains
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(Peer Reviewed Journal)
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Khalil, R., Skinner, C.B., Patfield, S.A., He, X. 2016. Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains. Pathogens and Disease. 74(5):1-11. doi: 10.1093/femspd/ftw037.
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Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum
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(Abstract Only)
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Wu, V.C., Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2016. Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum. ARS Food Safety and Inspection Service Research Workshop. ARS-FSIS.
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New monoclonal antibodies against a novel subtype of Shiga toxin 1 produced by Enterobacter cloacae and their use in analysis of human serum
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(Peer Reviewed Journal)
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Skinner, C.B., Patfield, S.A., Khalil, R., Kong, Q., He, X. 2016. New monoclonal antibodies against a novel subtype of Shiga toxin 1 produced by Enterobacter cloacae and their use in analysis of human serum. mSphere. 1(1):e00099-15.
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A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef
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(Peer Reviewed Journal)
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He, X., Kong, Q., Patfield, S.A., Skinner, C.B., Rasooly, R. 2016. A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef. PLoS One. 11(1):e0148092.
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Improved method for extraction of castor seed for toxin determination
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(Peer Reviewed Journal)
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McKeon, T.A., Brandon, D.L., He, X. 2015. Improved method for extraction of castor seed for toxin determination. Biocatalysis and Agricultural Biotechnology. 5:56-57. doi: 10.1016/j.bcab.2015.12.007.
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Analysis of castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA)
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(Peer Reviewed Journal)
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Brandon, D.L., McKeon, T.A., Patfield, S.A., He, X. 2016. Analysis of castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA). Journal of the American Oil Chemists' Society. 93:359-363.
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An environmental shiga toxin-producing Escherichia coli O145 clonal population exhibits high-level phenotypic variation that includes virulence traits
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(Peer Reviewed Journal)
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Carter, M.Q., Quinones, B., He, X., Louie, J.W., Zhong, W.W., Lee, B.G., Yambao, J.C., Mandrell, R.E., Cooley, M.B. 2015. An environmental shiga toxin-producing Escherichia coli O145 clonal population exhibits high-level phenotypic variation that includes virulence traits. Applied and Environmental Microbiology. doi: 10.1128/AEM.03172-15.
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Evaluation of castor oil samples for potential toxin contamination
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(Peer Reviewed Journal)
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Mckeon, T.A., Patfield, S.A., He, X. 2016. Evaluation of castor oil samples for potential toxin contamination. Industrial Crops and Products. 93:299-301.
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Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum
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(Peer Reviewed Journal)
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Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2015. Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum. Toxins. 7:5236-5253.
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Immuno-PCR assay for sensitive detection of proteins in real time
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He, X., Patfield, S.A. 2016. Immuno-PCR assay for sensitive detection of proteins in real time. Methods in Molecular Biology. 1318:139-148. doi: 10.1007/978-1-4939-2742-5_14.
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Serum Shiga toxin 2 values in patients during the acute phase of post-diarrheal hemolytic uremic syndrome
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(Peer Reviewed Journal)
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Serum Shiga toxin 2 values in patients during the acute phase of diarrhoea-associated hemolytic uremic syndrome
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(Peer Reviewed Journal)
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He, X., Quinones, B., Te Loo, M., Loos, S., Scavia, G., Brigotti, M., Levtchenko, E., Monnens, L. 2015. Serum Shiga toxin 2 values in patients during the acute phase of diarrhoea-associated hemolytic uremic syndrome. Acta Paediatrica. 104(12):e564-568.
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Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104:H4 outbreak strain
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(Peer Reviewed Journal)
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Kunsmann, L., Ruter, C., Bauwens, A., Greune, L., Gluder, M., Kemper, B., Fruth, A., Wai, S., He, X., Lloubes, R., Schmidt, A.M., Dobrindt, U., Mellmann, A., Karch, H., Bielaszewska, M. 2015. Virulence from vesicles: Novel mechanisms of host cell injury by Escherichia coli O104:H4 outbreak strain. Scientific Reports. doi: 10.1038/srep13252.
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An in vitro combined antibiotic/antibody treatment eliminates toxicity from Shiga toxin-producing E. coli
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(Peer Reviewed Journal)
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Skinner, C.B., Patfield, S.A., He, X., Zhang, G. 2015. An in vitro combined antibiotic/antibody treatment eliminates toxicity from Shiga toxin-producing E. coli. Antimicrobial Agents and Chemotherapy. 59(9):5435-5444.
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New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes
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(Peer Reviewed Journal)
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Skinner, C.B., Patfield, S.A., Hernlem, B.J., He, X. 2015. New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes. PLoS One. 10(7):e0132419.
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Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices
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(Peer Reviewed Journal)
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Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Hnasko, R.M. 2015. Rapid detection of E. coli produced shiga-like toxins by lateral flow immunoassay in multiple food matrices. Toxins. Toxins 7:1163-1173.
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Detection of shiga toxins by lateral flow assay
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(Peer Reviewed Journal)
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Ching, K.H., He, X., Stanker, L.H., Lin, A.V., Mcgarvey, J.A., Hnasko, R.M. 2015. Detection of shiga toxins by lateral flow assay. Toxins. 7:1163-1173. doi: 10.3390/Toxins7041163.
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Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin
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(Peer Reviewed Journal)
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Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2015. Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin. Toxins. 7(3):835-845. doi: 10.3390/toxins7030835.
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Castor diacylglycerol acyltransferase type1(DGAT1)displays greater
activity with diricinolein than Arabidopsis DGAT1
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(Peer Reviewed Journal)
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Mckeon, T.A., He, X. 2015. Castor diacylglycerol acyltransferase type1(DGAT1)displays greater activity with diricinolein than Arabidopsis DGAT1. Biocatalysis and Agricultural Biotechnology. 4:276-278.
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Toxin content of commercial castor cultivars
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(Peer Reviewed Journal)
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Mckeon, T.A., Auld, D., Brandon, D.L., Leviatov, S., He, X. 2014. Toxin content of commercial castor cultivars. Journal of the American Oil Chemists' Society. DOI: 10.1007/s11746-014-2505-3.
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A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins
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(Peer Reviewed Journal)
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Gehring, A.G., He, X., Fratamico, P.M., Lee, J., Bagi, L.K., Brewster, J.D., Paoli, G., He, Y., Xie, Y., Skinner, C.B., Barnett, C., Harris, D. 2014. A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins. Toxins. 6:1855-1872.
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New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo
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(Peer Reviewed Journal)
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Skinner, C.B., Patfield, S.A., Stanker, L.H., Fratamico, P.M., He, X. 2014. New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo. PLoS One. 9(6):e99854. DOI:10.1371journal.pone0099854.
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Current technologies for detection of ricin in different matrices
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He, X. 2014. Current technologies for detection of ricin in different matrices.Cherwonogrodky, J.W., editor. Ricin Toxin. Oak Park, Illinois:Bentham Science. p. 38-56.
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Risk of ricin from commercial castor production in North America
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Auld, D.L., Trostle, C.L., Miller, T.D., He, X., Duncan, R.W. 2014. Risk of ricin from commercial castor production in North America. Cherwonogrodky, J.W., editor. Ricin Toxin. Oak Park, Illinois:Bentham Science. p. 86-97.
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Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts
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(Peer Reviewed Journal)
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Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I., Hui, C., Patfield, S.A., Carter, J.M., He, X. 2014. Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts. Analytical Chemistry. 86(10):4698-4706.
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Electron-beam is effective in reducing Escherichia coli on blueberries and extending the shelf life of the fruits
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(Peer Reviewed Journal)
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Kong, Q., Wu, A., Qi, W., Qi, R., Carter, J.M., Rasooly, R., He, X. 2014. Electron-beam is effective in reducing Escherichia coli on blueberries and extending the shelf life of the fruits. Postharvest Biology and Technology. 95(2014):28-35.
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Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range.
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(Abstract Only)
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Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I.A., Hui, C., Patfield, S.A., He, X. 2014. Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range [abstract]. Meeting Abstract. Innovations in Mass Spectrometry. Paper No. 355.
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Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium
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(Peer Reviewed Journal)
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Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2014. Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium. Journal of Agricultural and Food Chemistry. 62:3301-3305 DOI.org/10.1021/jf500278a.
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Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies
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(Peer Reviewed Journal)
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Cheng, L.W., Henderson II, T.D., Patfield, S.A., Stanker, L.H., He, X. 2013. Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies. Infection and Immunity. 5:1845-1858. DOI: 10.3390/toxins5101845.
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Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f
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(Peer Reviewed Journal)
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Skinner, C.B., Patfield, S.A., Stanker, L.H., He, X. 2013. Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f. PLoS One. 8(9):e76563 DOI: 10.1371/journal/pone.0076563.
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A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples
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(Peer Reviewed Journal)
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He, X., Patfield, S.A., Hnasko, R.M., Rasooly, R., Mandrell, R.E. 2013. A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples. PLoS One. 8(10):e76368.
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Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells
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(Peer Reviewed Journal)
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Rasooly, R., Hernlem, B.J., He, X. 2013. Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells. Toxins. 5:1447-1461. doi:10.3390/toxins5081447.
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Detection of bacterial toxins by lateral flow immunoassay
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(Proceedings)
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Ching, K.H., Stanker, L.H., He, X., Hnasko, R.M. 2013. Detection of bacterial toxins by lateral flow immunoassay. p. 1-28. Irvine, CA; Biodot.
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Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2
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(Peer Reviewed Journal)
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Skinner, C.B., Patfield, S.A., Rasooly, R., Carter, J.M., He, X. 2013. Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2. PLoS One. 8(3):e59760.
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Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk
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(Peer Reviewed Journal)
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He, X., Mcmahon, S.A., Skinner, C.B., Merrill, P.A., Scotcher, M., Stanker, L.H. 2013. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. Journal of Immunological Methods. 389(1-2):18-28. doi:10.1016/j.jim.2012.12.005.
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Reducing the toxicity of castor seed meal through processing treatments
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(Peer Reviewed Journal)
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McKeon, T.A., Shim, K., He, X. 2012. Reducing the toxicity of castor seed meal through processing treatments. Biocatalysis and Agricultural Biotechnology. doi.org/10.1016/j.bcab.2012.12.001.
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A review on the challenges for increassed production of castor
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Severino, L.S., Auld, D.L., Baldanzi, M., Chen, G.Q., He, X., Crosby, W., Machado, O.L., Morris, J.B., Zieler, H. 2012. A review on the challenges for increassed production of castor. Agronomy Journal. 104(4):853-880.
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Replacement of animals in assays for detection of toxins in food(Abstract)
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(Abstract Only)
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Detoxification of castor meal through reactive seed crushing
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(Peer Reviewed Journal)
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Dubois, J., Piccirilli, A., Magne, J., He, X. 2012. Detoxification of castor meal through reactive seed crushing. Industrial Crops and Products. 43(1):194-199.
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Milk inhibits the biological activity of ricin
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(Peer Reviewed Journal)
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Rasooly, R., He, X., Friedman, M. 2012. Milk inhibits the biological activity of ricin. Journal of Biological Chemistry. 287(33):27924-27929. doi:10.1074/jbc.M112.362988.
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A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli
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(Peer Reviewed Journal)
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He, X., Quinones, B., Mcmahon, S.A., Mandrell, R.E. 2012. A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli. Toxins. 4(7):487-504. doi:10.3390/toxins4070487
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Sensitive bioassay for detection of biologically active ricin in food
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(Peer Reviewed Journal)
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Rasooly, R., He, X. 2012. Sensitive bioassay for detection of biologically active ricin in food. Journal of Food Protection. 75(5):951-954.
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Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum
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(Peer Reviewed Journal)
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He, X., Mcmahon, S.A., Rasooly, R. 2012. Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum. Biocatalysis and Agricultural Biotechnology. 1(2):105-109. doi:10.1016/j.bcab.2011.08.016.
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Sensitive detection of Shiga toxin 2 and some of its Stx 2 variants in environmental samples by a novel immuno-PCR assay
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(Peer Reviewed Journal)
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He, X., Qi, W., Quinones, B., Mcmahon, S.A., Cooley, M.B., Mandrell, R.E. 2011. Sensitive detection of Shiga toxin 2 and some of its Stx 2 variants in environmental samples by a novel immuno-PCR assay. Applied and Environmental Microbiology. 77(1):3558-3564. doi:10.1128/AEM.02205-10.
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Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR
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(Peer Reviewed Journal)
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He, X., Mcmahon, S.A., Henderson II, T.D., Griffey, S.M., Cheng, L.W. 2010. Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR. PLoS One. 5(9):e12858. doi:10.1371/journal.pone.0012858.
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Development of a Novel Immuno-PCR Assay for Detection of Ricin in Ground Beef, Liquid Chicken Egg and Milk
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(Peer Reviewed Journal)
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He, X., McMahon, S.A., McKeon, T.A., Brandon, D.L. 2010. Development of a Novel Immuno-PCR Assay for Detection of Ricin in Ground Beef, Liquid Chicken Egg and Milk. Journal of Food Protection. 73(4):695-700
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Simple method to detect triacylglycerol biosynthesis in a yeast-based recombinant system
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(Peer Reviewed Journal)
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Siloto, R.M., Truksa, M., He, X., McKeon, T.A., Weselake, R. 2009. Simple method to detect triacylglycerol biosynthesis in a yeast-based recombinant system. Lipids. 44:963-973.
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Detection of Protein Toxins
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(Abstract Only)
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Brandon, D.L., Cheng, L.W., He, X., Rasooly, R., Scotcher, M.C., Stanker, L.H., Carter, J.M. 2009. Detection of Protein Toxins. Meeting Abstract. Poster No. 8.
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Validation of a Cell-Free Translation Assay for Detecting Shiga Toxin 2 in Bacterial Culture
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(Peer Reviewed Journal)
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He, X., Quinones, B., Carter, J.M., Mandrell, R.E. 2009. Validation of a Cell-Free Translation Assay for Detecting Shiga Toxin 2 in Bacterial Culture. Journal of Agriculture and Food Chemistry. 57(11):5084-5088
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Detection and bioavailability of two biothreat toxins
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(Abstract Only)
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Brandon, D.L., Cheng, L.W., He, X., Rasooly, R., Scotcher, M.C., Stanker, L.H., Carter, J.M. 2008. Detection and bioavailability of two biothreat toxins. Meeting Abstract. Poster No. 2.
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Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods
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(Proceedings)
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Carter, J.M., Cheng, L.W., He, X., Rasooly, R., Brandon, D.L., Stanker, L.H. 2010. Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods. Proceedings from Biodetection Technologies 6th Edition. 5:75-95.
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Global challenges/chemistry solutions: Promoting personal safety and national security
()
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Effect of food matrices on the biological activity of ricin
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(Peer Reviewed Journal)
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He, X., Lu, S.S., Cheng, L.W., Rasooly, R., Carter, J.M. 2008. Effect of food matrices on the biological activity of ricin. Journal of Food Protection 71(10):2053-2058.
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Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay
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(Peer Reviewed Journal)
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Rasooly, R., Stanker, L.H., Carter, J.M., Do, P.M., Cheng, L.W., He, X., Brandon, D.L. 2008. In vitro peptide cleavage assay for detection of Botulinum Neurotoxin-A activity in food. International Journal of Food Microbiiology. 126 (2008):135-139
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Ricinus communis contains and acyl-CoA synthetase that preferentially activates ricinoleate to its CoA thioester
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(Peer Reviewed Journal)
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He, X., Chen, G.Q., Kang, S.T., Mckeon, T.A. 2007. Ricinus communis contains and acyl-CoA synthetase that preferentially activates ricinoleate to its CoA thioester. Lipids Journal. 42:931-938.
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Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs
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(Peer Reviewed Journal)
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He, X., Carter, J.M., Brandon, D.L., Cheng, L.W., Mckeon, T.A. 2007. Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs. Journal of Agricultural and Food Chemistry. 55:6897-6902
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Molecular Approaches for the Identification of Toxin in Foods
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(Abstract Only)
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Synthesis of Novel Acylglycerol Substrates for Acyltransferases.
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(Abstract Only)
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McKeon, T.A., Kang, S., He, X., Lin, J.T., Turner, C. 2007. Synthesis of Novel Acylglycerol Substrates for Acyltransferases.. Proceedings JOCS/AOCS World Congress.
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Expression Profiles of Genes Involved in Fatty Acid and Triacyblycerol Synthesis in Castor Bean (Ricinus communis L.)
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(Peer Reviewed Journal)
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Chen, G.Q., Turner, C., He, X., Nguyen, T.T., McKeon, T.A., Chingcuanco, D.L. 2007. Expression Profiles of Genes Involved in Fatty Acid and Triacyblycerol Syntheses in Castor Bean (Ricinus communis L.). Lipids. 41:263-274.
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Detection of castor contamination by real-time polymerase chain reaction
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(Peer Reviewed Journal)
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He, X., Brandon, D.L., Chen, G.Q., McKeon, T.A., Carter, J.M. 2007. Detection of castor contamination by real-time polymerase chain reaction. Journal of Agricultural and Food Chemistry. 55(2):545-550.
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Castor oil: Biosynthesis and Uses
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(Abstract Only)
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Mckeon, T.A., He, X., Chen, G.Q., Lin, J.T. 2007. Castor oil: Biosynthesis and Uses. 226th American Chemical Society National Meeting.
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The Enzymology of Castor Oil Biosynthesis
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(Abstract Only)
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McKeon, T.A., He, X., Chen, G.Q., Lin, J.T. 2006. The Enzymology of Castor Oil Biosynthesis. Association for the Advancement of Industrial Crops Conference.
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BIOAVAILABILITY OF CRUDE RICIN IN FOOD MATRICES
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(Abstract Only)
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Cheng, L.W., Brandon, D.L., He, X., Chen, G.Q., Carter, J.M. 2006. Bioavailability of crude ricin in food matrices. [Abstract]. ACS National Meeting and Exposition. Poster AGFD 64
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DEVELOPMENT OF MONOCLONAL ANTIBODIES SPECIFIC FOR RICIN
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(Abstract Only)
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Brandon, D.L., Cheng, L.W., He, X., Carter, J.M. 2006. Development of monoclonal antibodies specific for ricin. [Abstract]. ACS National Meeting and Exposition. Oral Presentation AGRO 234
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DETECTION OF CASTOR CONTAMINATION IN GROUND BEEF BY REAL-TIME PCR
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(Abstract Only)
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He, X., Brandon, D.L., Chen, G.Q., Mckeon, T.A., Carter, J.M. 2006. Detection of castor contamination in ground beef by real-time pcr. [Abstract]. ACS National Meeting and Exposition. Poster AGFD 18.
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DIACYLGLYCEROL ACYLTRANSFERASE ACTIVITY AND TRIACYLGLYCEROL SYNTHESIS IN GERMINATING CASTOR SEED COTYLEDONS
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(Peer Reviewed Journal)
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He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2006. Diacylglycerol acyltransferase activity and triacylglycerol synthesis in germinating castor seed cotyledons. Lipids. Vol. 41, Number 3: 281-285, 2006.
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TOWARD THE DEVELOPMENT OF A SAFE CASTOR CROP: EXPRESSION PATTERN COMPARISON OF RICIN AND 2S ALBUMIN GENES AND PHYLOGENY ANALYSIS OF 2S ALBUMINS
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(Peer Reviewed Journal)
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Chen, G.Q., He, X., Ahn, Y., Vang, L., Mckeon, T.A. 2006. Toward the development of a safe castor crop: expression pattern comparison of ricin and 2s albumin genes and phylogeny analysis of 2s albumins. Recent Progress in Medicinal Plants. 15: pp. 453-463
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SELECTIVE HYDROLYSIS OF POLAR TRIACYLGLYCEROLS BY LIPASES
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(Abstract Only)
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McKeon, T.A., Kang, S., Turner, C., He, X., Lin, J.W. 2005. Selective Hydrolysis of Polar Triacylglycerols by Lipases. Pacifichem Symposium.
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CASTOR OIL BOISYNTHESIS AND EXPRESSION PROFILES OF LIPID GENES IN DEVELOPING SEED OF RICINUS COMMUNIS L. (CASTOR BEAN)
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(Abstract Only)
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Chen, G.Q., Turner, C., He, X., Mckeon, T.A. 2005. Castor oil boisynthesis and expression profiles of lipid genes in developing seed of ricinus communis l. (castor bean). Meeting Abstract.
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QUANTITATIVE TRANSCRIPT PROFILING OF LIPID BIOSYNTHESIS GENES IN DEVELOPING CASTOR SEEDS.
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(Abstract Only)
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Chen, G.Q., Ahn, Y., He, X., Mckeon, T.A. 2005. Quantitative transcript profiling of lipid biosynthesis genes in developing castor seeds.. National Plant Lipid Cooperative Meeting.
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ENDOSPERM MORPHOLOGY AND TRANSCRIPT PROFILES OF STORAGE PROTEINS IN DEVELOPING CASTOR SEEDS.
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(Abstract Only)
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Chen, G.Q., Ahn, Y., He, X., Mckeon, T.A. 2005. Endosperm morphology and transcript profiles of storage proteins in developing castor seeds.. World Congress on In Vitro Biology.
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MOLECULAR CHARACTERIZATION OF THE ACYL-COA-DEPENDENT DIACYLGLYCEROL ACYLTRANSFERASE IN PLANTS
()
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He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2005. Molecular characterization of the acyl-coa-dependent diacylglycerol acyltransferase in plants. Research Signpost: Recent Research Developments in Applied Microbiology and Biotechnology. 2:69-86.
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EFFECT OF DIACYLGLYCEROL ACYLTRANSFERASE ON LIPID METABOLISM IN DEVELOPING SEEDS OF CASTOR
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(Abstract Only)
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He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2005. Effect of diacylglycerol acyltransferase on lipid metabolism in developing seeds of castor. Annual Meeting and Expo of the American Oil Chemists' Society.
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A SIMPLE AND SENSITIVE ASSAY FOR DISTINGUISHING THE EXPRESSION OF RICIN AND RICINUS COMMUNIS AGGLUTININ GENE IN DEVELOPING CASTOR SEED (RICINUS COMMUNIS L.)
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(Peer Reviewed Journal)
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Chen, G.Q., He, X., McKeon, T.A. 2005. A simple and sensitive assay for distinguishing the expression of ricin and ricinus communis agglutinin gene in developing castor seed (Ricinus communis L.). Journal of Agricultural and Food Chemistry. 53(6):2358-2361.
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REGULATION OF DIACYLGLYCEROL ACYLTRANSFERASE IN DEVELOPING SEEDS OF CASTOR
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(Peer Reviewed Journal)
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He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2005. Regulation of diacylglycerol acyltransferase in developing seeds of castor. Lipids. 39: pp. 865-871
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2S ALBUMIN GENE EXPRESSION IN CASTOR PLANT (RICINUS COMMUNIS L.)
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(Peer Reviewed Journal)
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Chen, G.Q., He, X., Liao, L., McKeon, T.A. 2004. 2s Albumin Gene Expression in Castor Plant (Ricinus communis L.). Journal of the American Oil Chemists' Society. 81(9):867-872.
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CLONING AND CHARACTERIZATION OF CASTOR DGAT
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(Peer Reviewed Journal)
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He, X., Chen, G.Q., Lin, J.T., Mckeon, T.A. 2004. Cloning and characterization of castor dgat. Lipids Journal.39:311-318
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APPLICATION OF LIPASES TO DEVELOP NOVEL ACYLTRANSFERASE SUBSTRATES
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(Proceedings)
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Mckeon, T.A., Turner, C., He, X., Chen, G.Q., Lin, J.T. 2003. Application of lipases to develop novel acyltransferase substrates. Meeting Abstract.
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ARS News Articles
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