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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Foodborne Toxin Detection and Prevention Research » Research » Publications at this Location » Publication #382344
Research Project: Technologies for the Detection of Bacterial and Plant Toxins and Allergens that Impact Food Safety and Food Defense

Location: Foodborne Toxin Detection and Prevention Research

Title: TMT-based quantitative proteomic and scanning electron microscopy reveals biological and morphological changes of Staphylococcus aureus irradiated by electron beam

Author
item ZHENG, QI - Shanghai Academy Of Agricultural Sciences
item CHEN, ZHIJUN - Shanghai Academy Of Agricultural Sciences
item YAN, WEIQIANG - Shanghai Academy Of Agricultural Sciences
item WANG, HAIHONG - Shanghai Academy Of Agricultural Sciences
item TIAN, WENHUI - Shanghai Academy Of Agricultural Sciences
item FENG, DONGSHENG - Shanghai Academy Of Agricultural Sciences
item YUE, LING - Shanghai Academy Of Agricultural Sciences
item QI, WENYUAN - Shanghai Academy Of Agricultural Sciences
item He, Xiaohua
item KONG, QIULIAN - Shanghai Academy Of Agricultural Sciences

Submitted to: LWT - Food Science and Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/10/2023
Publication Date: 6/12/2023
Citation: Zheng, Q., Chen, Z., Yan, W., Wang, H., Tian, W., Feng, D., Yue, L., Qi, W., He, X., Kong, Q. 2023. TMT-based quantitative proteomic and scanning electron microscopy reveals biological and morphological changes of Staphylococcus aureus irradiated by electron beam. LWT - Food Science and Technology. 184. Article 114977. https://doi.org/10.1016/j.lwt.2023.114977.
DOI: https://doi.org/10.1016/j.lwt.2023.114977

Interpretive Summary: According to CDC, Staphylococus aureus (S. aureus) is responsible for 33% of food poisoning in the United States, while S. enterotoxin A (SEA) is the major cause of staphylococcal food poisoning. Technologies effective in decontamination of this pathogen in food are needed. This study evaluated the influence of electron beam irradiation on the viability of S. aureus, its DNA and protein profiling and SEA expression in an attempt to determine the proper irradiation dose to be used for sterilization sufficient for viable microbial cells. The results obtained are important for future application of electron beam irradiation to decontaminate S. aureus in food.

Technical Abstract: To ensure food safety and reduce food poisoning, the D10 value, growth curve, Staphylococcal enterotoxin A (SEA) content, Staphylococcal enterotoxin A (sea) gene expression, protein and morphological changes of Staphylococcus aureus irradiated by electron beam were studied. The results showed that electron beam irradiation sensitized the strain to irradiation and prolonged the lag phase of bacterial growth. The SEA expression was induced significantly at 4 and 7 hours post irradiation at both transcriptional and translational levels. Moreover, differential protein expression profiles were revealed between irradiated and unirradiated bacterial through SDS-PAGE analysis. However, no noticeable morphological changes were observed in irradiated S. aureus. These findings provided important information in the basic characteristic changes of S. aureus after electron beam irradiation, which contributes to a better understanding of the effects of electron beam irradiation on food pathogens.