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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #343537

Research Project: Ecology and Detection of Human Pathogens in the Produce Production Continuum

Location: Produce Safety and Microbiology Research

Title: Complete Genome Sequence of a Shiga toxin-producing Enterobacter cloacae Clinical Isolate

item Carter, Michelle
item Pham, Antares
item Huynh, Steven
item He, Xiaohua

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/8/2017
Publication Date: 9/14/2017
Citation: Carter, M.Q., Pham, A.C., Huynh, S., He, X. 2017. Complete Genome Sequence of a Shiga toxin-producing Enterobacter cloacae Clinical Isolate. Genome Announcements. 5(37):e00883-17.

Interpretive Summary: Enterobacter cloacae is ubiquitous in nature and occurs as normal intestinal microflora in humans and animals. In recent years, it has been increasingly recognized as a major pathogen in nosocomial infections. Shiga toxins (Stxs) are cytotoxic proteins expressed mainly in enteric pathogens Shigella dysenteriae serotype 1 and Shiga toxin-producing Escherichia coli (STEC). In 2014, an E. cloacae strain expressing a novel subtype of Shiga toxin 1 (Stx1e) was isolated from a patient with mild diarrhea. To gain insight into the emergence of Stx-producing E. cloacae, we sequenced the complete genome of this Stx1e-producing E. cloacae strain. The genome is composed of a 4,918,273 bp chromosome and a 169,226 bp plasmid, encoding 4,711 CDSs, 25 rRNAs, and 88 tRNAs. Furthermore, five intact prophages were identified on the chromosome, including the Stx1e-converting prophage. This stx1e prophage is 40,210 bp in length, consisting of 39 phage related genes and 10 genes with unknown functions. The gene encoding Stx1e is located in the late gene region, implying the expression of stx1e is tightened with the prophage induction. The integration site recognized by this stx1e prophage is present in genomes of other bacterial strains, implying their potential in acquisition of stx1e. To our knowledge, this is the first report on a complete genome of Shiga toxin-producing E. cloacae. The genomic information revealed in this study provides molecular base for identification of natural niches that may harbor Shiga toxin positive bacterial strains.

Technical Abstract: Enterobacter cloacae strain M12X01451 was isolated from a patient with mild diarrhea. This strain produces a novel subtype of Shiga toxin 1, Stx1e. The Stx1e-converting prophage in strain M12X01451 is stable and can be transduced following induction. Here we report the complete genome sequence and annotation of strain M12X01451.