Page Banner

United States Department of Agriculture

Agricultural Research Service

Related Topics

Leslie Harden (Les)
Produce Safety and Microbiology Research
Chemist

Phone: (510) 559-5714
Fax: (510) 559-5777
Room 3100

USDA, ARS, PWA, WRRC-PSM
800 BUCHANAN STREET
ALBANY , CA 94710


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Quantification of toxins in soapberry (Sapindaceae) arils: Hypoglycin A and Methylenecyclopropylglycine -
Isenberg, S.L., Carter, M.S., Hayes, S.R., Graham, L., Johnson, D., Matthews, T.P., Harden, L.A., Takeoka, G.R., Thomas, J.D., Pirkle, J.L., Johnson, R.C. 2016. Quantification of toxins in soapberry (Sapindaceae) arils: Hypoglycin A and Methylenecyclopropylglycine. Journal of Agricultural and Food Chemistry. 64(27):5607-5613. doi: 10.1021/acs.jafc.6b02478.
Detection of methylenecyclopropylglycine (MCPG) and hypoglycin A in litchi fruit (abstract) -
Harden, L.A., Pan, J., Takeoka, G.R. 2015. Detection of methylenecyclopropylglycine (MCPG) and hypoglycin A in litchi fruit (abstract). 63rd ASMS Conference on Mass Spectrometry and Allied Topic, St. Louis, MO. May 31-June 4, 2015.
Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4 -
Zhang, Y., Du, W., Fregevu, C., Kothary, M.H., Harden, L.A., Mchugh, T.H. 2014. Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4. Journal of Agricultural and Food Chemistry. 62(52):12695-12700. doi: 10.1021/jf50451021.
Antioxidant activity, phenolic and anthocyanin contents of various rhubarb (Rheum-spp.) varieties Reprint Icon -
Takeoka, G.R., Dao, L.T., Harden, L.A., Pantoja, A., Kuhl, J.C. 2012. Antioxidant activity, phenolic and anthocyanin contents of various rhubarb (Rheum-spp.) varieties. Journal of Food Chemistry. DOI: 10.1111/j.1365-2621.2012.03174.x.
Isolation of Campylobacter from feral swine (Sus scrofa) on the ranch associated with the 2006 Escherichia coli O157:H7 spinach outbreak investigation in California Reprint Icon -
Jay-Russell, M.J., Bates, A.H., Harden, L.A., Miller, W.G., Mandrell, R.E. 2012. Isolation of Campylobacter from feral swine (Sus scrofa) on the ranch associated with the 2006 Escherichia coli O157:H7 spinach outbreak investigation in California. Zoonoses and Public Health. 10.1111/j.1863-2378.2012.01465.x.
Rapid Identification of Protein Biomarkers of E. coli O157:H7 by MALDI-TOF-TOF Mass Spectrometry and Top-Down Proteomics -
Fagerquist, C.K., Garbus, B.R., Miller, W.G., Williams, K.E., Yee, E., Bates, A.H., Boyle, S., Harden, L.A., Cooley, M.B., Mandrell, R.E. 2010. Rapid Identification of Protein Biomarkers of E. coli O157:H7 by MALDI-TOF-TOF Mass Spectrometry and Top-Down Proteomics. Analytical Chemistry. 82:2717-2725.
Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry -
Fagerquist, C.K., Garbus, B., Williams, K., Bates, A.H., Harden, L.A. 2010. Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry. Journal of American Society for Mass Spectrometry. 21,819-832.
Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry -
Fagerquist, C.K., Garbus, B.R., Williams, K.E., Bates, A.H., Boyle, S., Harden, L.A., Miller, W.G., Mandrell, R.E. 2009. Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry.IN: Proceedings of the 57th American Society of Mass Spectrometry Conference, May 31-June 4, 2009, Philadelphia, PA. DVD:A091307.8125ver.1.pdf
Web-based software for rapid "top-down" proteomic identification of protein biomarkers with implications for bacterial identification -
Fagerquist, C.K., Garbus, B.R., Williams, K., Bates, A.H., Boyle, S., Harden, L.A. 2009. Web-based software for rapid "top-down" proteomic identification of protein biomarkers with implications for bacterial identification. Applied and Environmental Microbiology. 75(13):4341-4353.
Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry -
Identification of acylglycerols containing dihydroxy fatty acids in castor (Ricinus communis L.)oil by mass spectrometry -
Lin, J.T., Arcinas, A.J., Harden, L.A. 2009. Identification of acylglycerols containing dihydroxy fatty acids in castor (Ricinus communis L.)oil by mass spectrometry. Lipids. 44:359-365.
SUB-SPECIATING CAMPYLOBACTER JEJUNI BY PROTEOMIC ANALYSIS OF ITS PROTEIN BIOMARKERS AND THEIR POST-TRANSLATIONAL MODIFICATIONS -
Fagerquist, C.K., Bates, A.H., Heath, S., King, B.C., Garbus, B.R., Harden, L.A., Miller, W.G. Sub-speciating campylobacter jejuni by proteomic analysis of its protein biomarkers and their post-translational modifications. Journal of Proteome Research. 5:10:2527-2538
IDENTIFICATION OF (12-RICINOLEOYL-RICINOLEOYL)-DIRICINOLEOYL-GYLCEROL, AN ACYLGLYCEROL CONTAINING FOUR ACYL CHAINS, IN CASTOR BY LC-ESI-MS -
Lin, J.T., Arcinas, A.J., Harden, L.A., Fagerquist, C.K. 2006. Identification of (12-ricinoleoyl-ricinoleoyl)-diricinoleoyl-gylcerol, an acylglycerol containing four acyl chains, in castor by lc-esi-ms. Journal of Agriculture and Food Chemistry. 54(10):3498-3504.
IDENTIFICATION OF (12-RICINOLEOYL-RICINOLEOYL)-DIRICINOLEOYL-GLYCEROL IN CASTOR OIL BY ESI-MS/MS AND ITS BIOSYNTHESIS -
Lin, J.T., Arcinas, A.J., Harden, L.A., Fagerquist, C.K. 2006. Identification of (12-ricinoleoyl-ricinoleoyl)-diricinoleoyl-glycerol in castor oil by esi-ms/ms and its biosynthesis. American Oil Chemists' Society Meeting.
IDENTIFICATION OF BENZALKONIUM CHLORIDE IN COMMERCIAL GRAPEFRUIT SEED EXTRACTS -
Takeoka, G.R., Dao, L.T., Wong, R.Y., Harden, L.A. 2005. Identification of Benzalkonium Chloride in Commercial Grapefruit Seed Extracts. Journal of Agricultural and Food Chemistry. 53:7630-7636.
GENOMIC AND PROTEOMIC IDENTIFICATION OF A DNA-BINDING PROTEIN USED IN THE "FINGERPRINTING" OF CAMPYLOBACTER SPECIES AND STRAINS BY MALDI-TOF-MS PROTEIN BIOMARKER ANALYSIS -
Fagerquist, C.K., Miller, W.G., Harden, L.A., Bates, A.H., Vensel, W.H., Wang, G., Mandrell, R.E. 2005. Genomic and proteomic identification of a dna-binding protein used in the "fingerprinting" of campylobacter species and strains by maldi-tof-ms protein biomarker analysis. Analytical Chemistry. web release 6/25/05, 77:4897-4907
SPECIATION OF CAMPYLOBACTER COLI, C. JEJUNI, C. HELVETICUS, C. LARI, C. SPUTORUM, AND C. UPSALIENSIS BY MATRIX-ASSISTED LASER DESORPTION IONIZATION-TIME OF FLIGHT (MALDI-TOF) MASS SPECTROMETRY -
Mandrell, R.E., Harden, L.A., Bates, A.H., Miller, W.G., Haddon, W.F., Fagerquist, C.K. 2005. Speciation of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis by matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry. Applied and Environmental Microbiology. 71:10 p. 6292-6307
DELPHINIDIN 3-O-(2-O-BETA-D-GLUCOPYRANOSYL-ALPHA-L-ARABINOPYRANOSIDE), A NOVEL ANTHOCYANIN IDENTIFIED IN BELUGA BLACK LENTILS -
Takeoka, G.R., Dao, L.T., Tamura, H., Harden, L.A. 2005. Delphinidin 3-O-(2-O-Beta-D-Glucopyranosyl-Alpha-L-arabinopyranoside): A Novel Anthocyanin Identified in Beluga Black Lentils. Journal of Agricultural and Food Chemistry. Vol 53. p. 4932-4937.
AUTHENTICATION OF COMMERCIAL GRAPEFRUIT SEED EXTRACTS -
Takeoka, G.R., Dao, L.T., Wong, R.Y., Harden, L.A. 2005. Authentication of commercial grapefruit seed extracts. Meeting Abstract. AFGD Paper No. 50, ACS Nat'l Meeting, March 2005, San Diego, CA.
FINGERPRINTING OF CAMPYLOBACTER SPECIES AND STRAINS BY MASS SPECTROMETRIC IDENTIFICATION OF PROTEIN BIOMARKERS -
Fagerquist, C.K., Harden, L.A., Miller, W.G., Fouts, D., Nelson, K., Mandrell, R.E. 2004. Fingerprinting of campylobacter species and strains by mass spectrometric identification of protein biomarkers. In: Proceedings of the 52nd American Society for Mass Spectrometry Conference and Allied Topics. May 23-27, 2004 Nashville, TN. CDROM
BIOCHEMICAL DIVERSITY AMONG THE 1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE ISOZYMES ENCODED BY THE ARABIDOPSIS GENE FAMILY -
YAMAGAMI, T., TSUCHISAKA, A., YAMADA, K., HADDON, W.F., HARDEN, L.A., THEOLOGIS, A. Biochemical Diversity among the 1-Amino-cyclopropane-1-Carboxylate Synthase Isozymes Encoded by the Arabidopsis Gene Family. JOURNAL OF BIOLOGICAL CHEMISTRY. 2003. V. 278:49102-49112.
LIPASE-CATALYZED SYNTHESIS OF 1,2(2,3)-DIRICINOLEIN. -
TURNER, C., NGUYEN, T.T., LIN, J.T., WONG, R.Y., HARDEN, L.A., LUNDIN, R.E., MCKEON, T.A. LIPASE-CATALYZED SYNTHESIS OF 1,2(2,3)-DIRICINOLEIN.. BIOCHEMISTRY & MOLECULAR BIOLOGY OF PLANT FATTY ACIDS AND GLYCEROLIPIDS. LIPIDS, v. 38(11)2003, pp:1197-1205
Last Modified: 5/2/2016
Footer Content Back to Top of Page