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United States Department of Agriculture

Agricultural Research Service

Related Topics

Camille M Steber

Research Plant Molecular Geneticist


The Steber Laboratory is part of the Wheat Health, Genetics, and Quality Research Unit, United States Department of Agriculture, Agricultural Research Service, and is housed in and affiliated with the Department of Crop and Soil Sciences, at Washington State University in Pullman, Washington. The principal investigator is Camille Steber, and the lab was started in 1998.

 The Steber Laboratory is focused on the study of hormonal control of seed dormancy and germination, as well as plant stress responses. Basic research projects are carried out in the Arabidopsis thaliana model system, and knowledge from Arabidopsis is translated into improved resista/ARSUserFiles/20903500/images/Vogel and Steber Lab.jpgnce to preharvest sprouting and drought tolerance in wheat. Seed dormancy and germination are controlled by the balance between the abscisic acid (ABA) and gibberellin (GA) hormone signalling pathways. ABA induces seed dormancy during embryo maturation, whereas GA stimulates seed germination and is associated with dormancy breaking treatments such as cold stratification and dry after-ripening. Research in Arabidopsis is focused on understanding the mechanisms of GA signaling, and the relative roles of ABA and GA in the control of seed dormancy, dormancy loss, and germination. In addition to stimulating seed germination, GA also stimulates stem elongation, the transition to flowering, and is required for full fertility. Research in wheat is aimed at increasing preharvest sprouting (PHS) and drought tolerance using increased ABA sensitivity.

 

The Steber Laboratory uses mutation breeding as a tool for wheat improvement. The main effort uses wheat mutants with altered response to the hormone ABA to control wheat grain dormancy and drought tolerance. Wheat production is subject to risk of yield loss due to drought and to preharvest sprouting (PHS), the germination of mature grain (caryopses) on the mother plant when cool moist conditions persist close to harvest time. When PHS occurs grain must be sold as feed, causing considerable economic losses to farmers. PHS is associated with lack of grain dormancy and sensitivity to the plant hormone abscisic acid (ABA) in wheat. ABA stimulates dormancy as well as adaptive responses to drought, cold and salt stress. Our research examines whether higher PHS tolerance is associated with higher ABA sensitivity or decreased GA sensitivity. Another major effort is a collaborative project with Dr. Kimberly Garland Campbell and Dr. Scot Hulbert to improve wheat drought tolerance through multiple mechanisms such as increased water use efficiency, mobilization of water-soluble carbohydrates, and altered root architecture. In an o/ARSUserFiles/20903500/images/Steber, Tractor, Greenhouse.jpgngoing collaboration with Dr. Kimberlee K. Kidwell and Dr. Ian Burke, the laboratory is helping to analyze wheat variants with increased resistance to the herbicide glyphosate. In the past, the laboratory made contributions to improving Rhizoctonia root rot tolerance, tissue culture, and developed a floral dip method for wheat transformation. See the publications page for more information on our work. More information about the lab can be found here: http://public.wsu.edu/~csteber/.

 

 

 

 

 

 

 

 

 

 

 

Publications

(Clicking on the reprint iconReprint Icon will take you to the publication reprint.)
Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitive sly1-2 mutant of Arabidopsis thaliana-(Peer Reviewed Journal)
Nelson, S.K., Steber, C.M. 2017. Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitive sly1-2 mutant of Arabidopsis thaliana. PLoS One. 12:(6)e0179143.
Avoiding Low Falling Numbers Problems in Wheat-()
Steber, C.M. 2017. Avoiding Low Falling Numbers Problems in Wheat. Crops and Soils. Vol. 50, Issue 2, pages 22-25.
Extension Facts: Managing the risk of low falling numbers in wheat-()
Steber, C.M. 2016. Extension Facts: Managing the risk of low falling numbers in wheat. WSU Extension Bulletin. FS242E.
The wheat ABA hypersensitive ERA8 mutant is associated with increased preharvest sprouting tolerance, altered hormone sensitivity and accumulation-(Peer Reviewed Journal)
Martinez, S.A., Tuttle, K.M., Takebayashi, Y., Seo, M., Garland Campbell, K.A., Steber, C.M. 2016. The wheat ABA hypersensitive ERA8 mutant is associated with increased preharvest sprouting tolerance, altered hormone sensitivity and accumulation. Euphytica. doi:10.1007/s10681-016-1763-6.
The wheat ABA hypersensitive ERA8 mutant is associated with increased preharvest sprouting tolerance and altered hormone accumulation-(Peer Reviewed Journal)
Martinez, S., Tuttle, K.M., Takebayashi, Y., Seo, M., Garland Campbell, K.A., Steber, C.M. 2016. The wheat ABA hypersensitive ERA8 mutant is associated with increased preharvest sprouting tolerance and altered hormone accumulation. Euphytica. doi:10.1007/s10681-016-1763-6.
Gibberellin hormone signal perception: down-regulating DELLA repressors of plant growth and development-()
Nelson, S.K., Steber, C.M. 2016. Gibberellin hormone signal perception: down-regulating DELLA repressors of plant growth and development. Annual Plant Reviews. 49:153-188.
Molecular and phylogenetic characterization of the homoeologous EPSP Synthase genes of allohexaploid wheat, Triticum aestivum (L.)-(Peer Reviewed Journal)
Aramak, A., Kidwell, K., Steber, C.M., Burke, I. 2015. Molecular and phylogenetic characterization of the homoeologous EPSP Synthase genes of allohexaploid wheat, Triticum aestivum (L.). Biomed Central (BMC) Genomics. 16:844.
Grain dormancy loss is associated with changes in ABA and GA sensitivity and hormone accumulation in bread wheat, Triticum aestivum (L.)-(Peer Reviewed Journal)
Tuttle, K.M., Martinez, S., Schramm, E.C., Takebayashi, Y., Seo, M., Steber, C.M. 2015. Grain dormancy loss is associated with changes in ABA and GA sensitivity and hormone accumulation in bread wheat, Triticum aestivum (L.). Seed Science Research. 25(2):179-193.
Registration of Zak ERA8 soft white spring wheat germplasm with enhanced response to ABA and increased seed dormancyReprint Icon -(Peer Reviewed Journal)
Martinez, S., Schramm, E.C., Harris, T.J., Kidwell, K.K., Garland Campbell, K.A., Steber, C.M. 2014. Registration of Zak ERA8 soft white spring wheat germplasm with enhanced response to ABA and increased seed dormancy. Journal of Plant Registrations. 8(2)217-220. DOI: 10.3198/jpr2013.09.0060crg.
The roles of the GA receptors GID1a, GID1b, and GID1c in sly1-independent GA signalingReprint Icon -(Peer Reviewed Journal)
Hauvermale, A., Ariizumi, T., Steber, C.M. 2014. The roles of the GA receptors GID1a, GID1b, and GID1c in sly1-independent GA signaling. Plant Signaling and Behavior. 9:e28030; PMID: 24521922.
Lifting DELLA repression of Arabidopsis seed germination by nonproteolytic gibberellin signalingReprint Icon -(Peer Reviewed Journal)
Ariizumi, T., Hauvermale, A.L., Nelson, S.K., Hanada, A., Yamaguchi, S., Steber, C.M. 2013. Lifting DELLA repression of Arabidopsis seed germination by nonproteolytic gibberellin signaling. Plant Physiology. 162(4):2125-39. doi: 10.1104/pp.113.219451.
Increased ABA sensitivity results in higher seed dormancy in soft white spring wheat cultivar ‘Zak’-(Peer Reviewed Journal)
Schramm, E.C., Nelson, S.K., Kidwell, K.K., Steber, C.M. 2012. Increased ABA sensitivity results in higher seed dormancy in soft white spring wheat cultivar ‘Zak’. Theoretical and Applied Genetics. 126(3):791-803 Available: http://link.springer.com/article/10.1007/s00122-012-2018-0/fulltext.html.
Gibberellin signaling: a theme and variations on DELLA repression-()
Hauvermale, A.L., Ariizumi, T., Steber, C.M. 2012. Gibberellin signaling: a theme and variations on DELLA repression. Plant Physiology. 160:83-92 Available: http://www.plantphysiol.org/content/160/1/83.
Wheat ABA-insensitive mutants result in reduced grain dormancy-(Peer Reviewed Journal)
Schramm, E., Nelson, S.K., Steber, C.M. 2012. Wheat ABA-insensitive mutants result in reduced grain dormancy. Euphytica. 188(1),35-49. Published online March 31, 2012.
Concerted action of two avirulent spore effectors activates Reaction to Puccinia graminis 1 (Rpg1)-mediated cereal stem rust resistance-(Peer Reviewed Journal)
Nirmala, J., Drader, T., Lawrence, P.K., Yin, C., Hulbert, S., Steber, C.M., Steffenson, B.J., Szabo, L.J., Von Wettstein, D., Kleinhofs, A. 2011. Concerted action of two avirulent spore effectors activates Reaction to Puccinia graminis 1 (Rpg1)-mediated cereal stem rust resistance. Proceedings of the National Academy of Sciences. 108(35):14676-81.
Mutations in the F-box gene SNEEZY result in decreased arabidopsis GA signaling-(Peer Reviewed Journal)
Ariizumi, T., Steber, C.M. 2011. Mutations in the F-box gene SNEEZY result in decreased arabidopsis GA signaling. Plant Signaling and Behavior. 6(6):831-833.
Investigating the role of ABA signaling in wheat drought toleranceReprint Icon -(Abstract Only)
Pan, W., Schramm, E., Murphy, L.R., Garland Campbell, K.A., Steber, C.M. 2011. Investigating the role of ABA signaling in wheat drought tolerance. Meeting Abstract. www.intl-pag.org.
The role of two F-box proteins, SLEEPY1 and SNEEZY, in arabidopsis GA signaling-(Peer Reviewed Journal)
Ariizumi, T., Lawrence, P.K., Steber, C.M. 2011. The role of two F-box proteins, SLEEPY1 and SNEEZY, in arabidopsis GA signaling. Plant Physiology. 115:765-775.
Gibberellin Signaling: a Wake-up Call for Seed GerminationReprint Icon -(Abstract Only)
Ariizumi, T., Steber, C.M. 2010. Gibberellin Signaling: a Wake-up Call for Seed Germination. Meeting Abstract. www.york.ac.uk/org/cnap/ISSS/ISSSseeddormancy.html.
Isolation of ABA-responsive mutants in allohexaploid bread wheat (Triticum aestivum L.): Drawing connections to grain dormancy, preharvest sprouting, and drought toleranceReprint Icon -(Peer Reviewed Journal)
Schramm, E.C., Abellera, J.C., Strader, L., Garland Campbell, K.A., Steber, C.M. 2010. Isolation of ABA-responsive mutants in allohexaploid bread wheat (Triticum aestivum L.): Drawing connections to grain dormancy, preharvest sprouting, and drought tolerance. Plant Science. 179:620-629.
Isolation of ABA hypersensitive mutants in allhexaploid breadwheat-(Peer Reviewed Journal)
Abellera, J.C., Strader, L.C., Schramm, E., Garland Campbell, K.A., Steber, C.M. 2010. Isolation of ABA hypersensitive mutants in allhexaploid breadwheat. Genetics. 179:620-629.
Relieving DELLA Repression of Stem Elongation and Flowering, Evidence for a Proteolysis Independent Mechanism for GA signalingReprint Icon -(Abstract Only)
Ariizumi, T., Steber, C.M. 2009. Relieving DELLA Repression of Stem Elongation and Flowering, Evidence for a Proteolysis Independent Mechanism for GA signaling. Meeting Abstract. www.aspb.org/meetings/pb-2009.
Rhizoctonia Resistant Wheat -- Potential New Resources for Control for Soilborne Pathogens.-(Abstract Only)
Evidence for stable transformation of wheat by floraldip in Agrobacterium tumefaciensReprint Icon -(Peer Reviewed Journal)
Zale, J., Agarwal, S., Loar, S., Steber, C.M. 2009. Evidence for stable transformation of wheat by floraldip in Agrobacterium tumefaciens. Plant Cell Reports 28(6):903-913. DOI 10.1007/s00299-009-0696-0.
EMS-treated hexaploid wheat genotype Scarlet has enhanced tolerance to the soilborne necrotrophic pathogens Rhizoctonia solani AG-8 and R. oryzae. 2009. Theor. Appl. Genet. 119(February): 293-303-(Peer Reviewed Journal)
Okubara, P.A., Steber, C.M., Demacon, V.L., Walter, N., Paulitz, T.C., Kidwell, K.K. EMS-treated hexaploid wheat genotype Scarlet has enhanced tolerance to the soilborne necrotrophic pathogens Rhizoctonia solani AG-8 and R. oryzae. 2009. Theor. Appl. Genet. 119(February): 293-303. Theoretical and Applied Genetics.
Chapter 6: Floral Transformation of Wheat-()
Zale, J., Agarwal, S., Loar, S., Steber, C.M. 2008. Floral Transformation of Wheat, Chapter 6, pp. 105-113. IN: Huw D. Jones and Peter R. Shewry (eds.) Methods in Molecular Biology, Transgenic Wheat, Barley and Oats. Humana Press, New York, NY.
Controlling Soilborne Pathogens in Wheat Production Systems-(Abstract Only)
Okubara, P.A., Schroeder, K.L., Paulitz, T.C., Steber, C.M., Kidwell, K. 2008. Controlling Soilborne Pathogens in Wheat Production Systems. Washington State University College of Agriculture and Home Economics. Page 37.
EMS-generated Rhizoctonia resistance in an adapted wheat-(Abstract Only)
Okubara, P.A., Steber, C.M., Paulitz, T.C., Kidwell, K.K. 2008. Ems-generated rhizoctonia resistance in an adapted wheat. Journal of Plant Pathology. S.350.(2)
Proteolysis-independent down-regulation of DELLA repression by the gibberellin receptor GID1-(Peer Reviewed Journal)
Ariizumi, T., Murase, K., Sun, T.P., Steber, C.M. 2008. Proteolysis-independent down-regulation of DELLA repression by the gibberellin receptor GID1. The Plant Cell, 20(9):2447-59.
Molecular aspects of seed dormancy-()
Finkelstein, R., Reeves, W., Ariizumi, T., Steber, C.M. 2008. Molecular aspects of seed dormancy. Annu. Rev. Plant Biol. 59:387-415.
ABA And GA Signaling, Bridging The Gap Between Wheat And Arabidopsis.-(Abstract Only)
Schramm, E., Abellera, J., Strader, L.C., Steber, C.M. 2008. Aba and ga signaling, bridging the gap between wheat and arabidopsis.. Plant and Animal Genome Abstracts, Jan 12-16, San Diego, CA. W226.
ABA AND GA SIGNALING, BRIDGING THE GAP BETWEEN WHEAT AND ARABIDOPSIS-(Abstract Only)
Steber, C.M., Schramm, E., Abellera, J., Strader, L.C. 2007. Aba and ga signaling, bridging the gap between wheat and arabidopsis. Translational Seed Biology: From Model Systems to Crop Improvement, UC Davis, CA, Sept. 12-20. Invited Presentation.
RGL2 PROTEIN DOES NOT DISAPPEAR DURING SLY1 MUTANT SEED GERMINATION-(Abstract Only)
Ariizumi, T., Steber, C.M. 2007. Rgl2 protein does not disappear during sly1 mutant seed germination. International Conference on Plant Growth Substances Proceedings, July 21-25, Puerta Vallerta, Mexico, MS705.
A COMPARATIVE STUDY OF TWO F-BOX PROTEINS, SLEEPY1 AND SNEEZY IN GA SIGNALING-(Abstract Only)
Ariizumi, T., Steber, C.M. 2007. A comparative study of two f-box proteins, sleepy1 and sneezy in ga signaling. International Conference on Plant Growth Substances Proceedings, July 21-25, Puerta Vallerta, Mexico, PS1128.
Seed germination of GA-insensitive sleepy1 mutants does not require RGL2 protein disappearance in Arabidopsis-(Peer Reviewed Journal)
Ariizumi, T., Steber, C.M. 2007. Seed germination of GA-insensitive sleepy1 mutants does not require RGL2 protein disappearance in Arabidopsis. The Plant Cell 19, 791-804.
The role of the GA signaling SLY1 in Arabidopsis seed germination-(Abstract Only)
Steber, C.M. 2006. The role of the GA signaling SLY1 in Arabidopsis seed germination. Seed Biology: Advances and Applications. W1168 Seed Biology Group, San Antonio, TX, Dec 1-4, pg. 127.
UBIQUITIN BECOMES UBIQUITOUS IN GA SIGNALING -()
Ariizumi, T., Steber, C.M. 2006. Ubiquitin becomes ubiquitous in GA signaling, In: Taiz, L. and Zeiger, E., editors. Plant Physiology 4th Edition, Sunderland, MA: Sinauer Associates. 20:20.3.
THE ROLE OF THE SLEEPY1 (SLY1) F-BOX GENE IN GA REGULATION OF SEED GERMINATION IN ARABIDOPSIS -(Abstract Only)
Ariizumi, T., Schramm, E., Steber, C.M. 2006. The role of the sleepy1 (sly1) f-box gene in ga regulation of seed germination in arabidopsis. Arabidopsis Res Intern Conference Proceedings. June 28-July 2, Madison, WI. Abst #378.
CHAPTER 11, DE-REPRESSION OF SEED GERMINATION BY GA SIGNALING-()
Steber, C.M. 2006. Chapter 11: De-repression of seed germination by ga signaling. In: Seed Development, Dormancy and Germination. Eds. Kent Bradford and Hiroyuki Nonogaki. pgs 248-263. Blackwell Publishing, London, UK.
GIBBERELLIN METABOLISM AND SIGNALING-()
Thomas, S.G., Rieu, I., Steber, C.M. 2005. Gibberellin metabolism and signaling. Chapter 9 In: Plant Hormones; Vitamins and Hormones Advances in Research and Applications. Ed. Gerald Litwack. Elsevier Publishing, Boston, MA. 72:289-339.
RECESSIVE-INTERFERING MUTATIONS IN THE GIBBERELLIN SIGNALING GENE SLEEPY1 ARE RESCUED BY OVEREXPRESSION OF ITS HOMOLOGUE SNEEZY-(Peer Reviewed Journal)
Strader, L., Ritchie, S., Soule, J.D., Mcginnis, K.M., Steber, C.M. 2004. Recessive-interfering mutations in the ga-response gene sleepy1 are rescued by overexpression of its homologue sneezy. Proceedings of the National Academy of Sciences. 101:12771-12776.
THE ARABIDOPSIS F-BOX PROTEIN SLEEPY1 TARGETS GIBBERELLIN SIGNALING REPRESSORS FOR GIBBERELLIN-INDUCED DEGRADATION-(Peer Reviewed Journal)
Dill, A., Thomas, S.G., Hu, J., Steber, C.M., Sun, T.P. 2004. The arabidopsis f-box protein sleepy1 targets gibberellin signaling repressors for gibberellin-induced degradation. The Plant Cell. 16:1392-1405.
THE ORGANIZATION AND RATE OF EVOLUTION OF THE WHEAT TRANSCRIPTOME ARE CORRELATED WITH RECOMBINATION RATES ALONG CHROMOSOME ARMS-(Peer Reviewed Journal)
AKHUNOV, E.D., LAZO, G.R., CHAO, S., ANDERSON, O.D., GUSTAFSON, J.P., WALKER-SIMMONS, M.K., STEBER, C.M. THE ORGANIZATION AND RATE OF EVOLUTION OF THE WHEAT TRANSCRIPTOME ARE CORRELATED WITH RECOMBINATION RATES ALONG CHROMOSOME ARMS. GENOME RESEARCH. 2003. V. 13(5). P. 753-763.

Projects

Genetic Improvement of Wheat and Barley for Resistance to Biotic and Abiotic Stresses
In-House Appropriated (D)
 Accession Number:424575

 

           

 


Last Modified: 3/22/2017
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