Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 9/22/1998
Publication Date: N/A
Technical Abstract: Fusarium wilt caused by Fusarium oxysporum f. sp. melonis is one of the most devastating diseases in melon production worldwide. Efficient control measures for this soil-borne pathogen other than using resistant varieties are not available. Tagging resistance genes with molecular markers facilitates the cloning of the gene and marker-assisted selection in a breeding program. We have constructed a genetic map with 200 AFLP markers in order to identify these resistance genes in the genome. We have screened resistant and susceptible bulks constructed from backcross and F2 progenies using AFLP techniques. Markers potentially linked to Fom-2 (responsible for resistance to races 0 and 1 of the fungus) have been identified. Genomic Southern using five different restriction enzymes also showed that these markers represent low- or single-copy DNA sequences in the genome. This indicates the markers can be used as probes for physical mapping. For this purpose, a large insert genomic library of a multi-disease resistant melon line is under construction.