Submitted to: Biotechniques
Publication Type: Peer reviewed journal
Publication Acceptance Date: 10/10/1998
Publication Date: N/A
Citation: Interpretive Summary: The subject of this paper pertains to the rapidly-progressing field of biocontrol agent formulations, specifically, the preparation of a product (granules) for application. Solid-state fermentation of the biocontrol agent was combined innovately with extrusion. This new methodology resulted in improvement of the biocontrol agent's survival and because the most expensive part of a biocontrol product is the biocontrol agent, this association of solid-state fermentation and extrusion has economic impact and should be of commercial interest. This research will benefit the farmers and the herbicide industries.
Technical Abstract: Five fungal biocontrol agents useful in agriculture were grown on rice flour in plastic bags. The flour, infested with conidia and/or mycelia, was mixed with wheat flour, kaolin, and water and extruded into granules. The inoculum survived extrusion and fluid bed drying at 50C 3-92 times better than inoculum produced in liquid fermentation. Depending on the agent, the high level of flour infestation permitted a 1:9 to 1:1600 dilution to yield the 1x106 colony forming units (cfu)/g in the final product which is usually needed for biocontrol efficacy.