Submitted to: Journal of Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/25/1994
Publication Date: N/A
Interpretive Summary: Economically important traits of lentil were studied using a series of genetically defined lines from eight interspecific crosses. The traits studied included days to flower, days to maturity, plant height, biomass, seed yield, harvest index and seed weight. Loci (genes) that affect these traits were identified with the aid of molecular markers (isozymes) that had previously been located in the lentil genome. The information gained in the study indicates specific locations for genes which affect these traits. By association with the molecular markers it is now possible to manipulate the expression of those genes and to develop improved strains (varieties).
Technical Abstract: Polymorphism at isozyme loci was used to locate factors responsible for variation in quantitative traits of lentil. 8 sets of random single seed descent (RSSD) derived lines were developed by advancing individual F3 plants of interspecfic (L. culinaris Medik. X L. orientalis Boiss.) hybrids to the F6. The RSSD lines in each of the eight sets differed for alleles at 2-8 isozyme loci. In each set, association of isozyme loci with variation in seven quantitative traits (days to flower, days to mature, plant height, biomass, seed yield, harvest index, seed weight) was determined for each pairwise combination of a quantitative trait with a marker locus. Loci affecting variation in all seven quantitative traits were detected by their association with 14 isozyme markers (Aat-c, Aat-m, Aat-p, Adh-1, Fk, Gal-1, Gal-2, Lap-1, Lap-2, Pgd-p, Pgi, Pgm-c, Pgm-p, Skdh). The known position of 10 of the 14 isozyme loci on the lentil genetic map was used to mark the genomic regions for possible location of associated quantitative trait loci (QTL). Detected QTL were found to be located in six of the seven linkage groups on lentil genetic map. Regions of the genome represented by linkage groups 1, 5, and 7 appeared to affect a greater number of traits than other genomic regions represented by linkage groups 2, 3, and 4. Results indicated that the mean expression of quantitative traits at segregating marker locus classes can be used to locate the genetic factors in lentil which influence the behavior of economically important traits.